LOCUS       sequence-388289-       16500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 adenine base editing plasmid V1.0 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16500)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 16500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL672
FEATURES             Location/Qualifiers
     source          1..16500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6667..6687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6700..6720
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6733..6753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6764..7012
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(7274..7579)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7376..7395)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8062..8086)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8116..8140
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9441..10070
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10499..11572
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11638..11832
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12068..12090)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12166..12306
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12221..12240
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12321..12338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12492..13080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12572..12591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13167..13961)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYDELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14386..14410
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    14488..14662
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15752..16428)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15795..15815)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat gtctgaagtg
     2041 gagttctccc acgagtattg gatgaggcac gctcttacac ttgctaaaag ggctagggac
     2101 gaaagggaag ttccagttgg agctgttctc gtgctcaata acagggtgat tggcgagggt
     2161 tggaatagag ccattggact ccatgatcca acagctcacg cagagattat ggctcttaga
     2221 caaggcggcc tcgttatgca gaattacaga ctcatcgacg ccacactcta cgttaccttc
     2281 gaaccttgcg ttatgtgcgc cggagctatg atccattcta ggattggcag ggtcgtgttc
     2341 ggcgttagaa acgctaagac aggagctgca ggctctctta tggacgttct tcattaccca
     2401 ggcatgaatc atagagtgga gatcacagaa ggcattcttg cagacgagtg cgcagctctc
     2461 ctttgctatt tcttcaggat gccgaggcaa gttttcaacg ctcagaagaa ggcccagtct
     2521 tctacagatt ccggcggatc ttctggagga tctagcggag gatctatgac aaagctgaac
     2581 cagccatacg gcatcggcct cgctattggc tcaaactcta tcggcttcgc ggtggtcgac
     2641 gccaattccc acctcctgcg cctgaagggc gagacagcca tcggcgccag gctcttccgc
     2701 gagggacagt cagccgcgga tcggaggggc tctaggacca cacggaggcg cctctcccgg
     2761 acaaggtggc gcctcagctt cctgcgcgac ttcttcgcgc cacatatcac caagattgac
     2821 cctgatttct tcctccgcca gaagtactcc gagatcagcc ctaaggacaa ggatcggttc
     2881 aagtacgaga agaggctgtt caacgaccgc accgatgccg agttctacga ggattaccca
     2941 tcaatgtacc acctgaggct ccatctgatg acccacacac ataaggccga cccgagggag
     3001 attttcctcg ccatccacca tattctcaag tctcggggcc acttcctcac acctggagcg
     3061 gccaaggatt tcaataccga caaggtggat ctggaggaca tcttcccagc cctcacagag
     3121 gcgtacgccc aggtctaccc ggacctggag ctgaccttcg acctcgcgaa ggccgacgat
     3181 ttcaaggcga agctcctgga tgagcaggcc accccgtccg acacacagaa ggcgctggtg
     3241 aacctcctgc tctccagcga tggcgagaag gagattgtca agaagaggaa gcaggtgctg
     3301 acagagttcg cgaaggccat caccggcctc aagacaaagt tcaacctcgc cctgggcacc
     3361 gaggtggatg aggccgatgc ctcaaattgg cagttctcta tgggccagct cgacgataag
     3421 tggtccaaca tcgagacatc catgacagac cagggcaccg agattttcga gcagattcag
     3481 gagctgtacc gggcgaggct gctcaacggc attgtgccag ccggcatgtc cctgagccag
     3541 gccaaggtcg ccgattacgg ccagcacaag gaggacctgg agctgttcaa gacatacctc
     3601 aagaagctga atgatcatga gctggcgaag accatccggg gcctctacga caggtacatt
     3661 aacggcgacg atgccaagcc gttcctgcgc gaggacttcg tcaaggcgct caccaaggag
     3721 gtgacagccc atccaaacga ggtgtccgag cagctgctca acaggatggg ccaggccaat
     3781 ttcatgctca agcagaggac caaggccaac ggcgcaatcc caattcagct ccagcagcgc
     3841 gagctggatc agatcattgc gaaccagagc aagtactacg actggctcgc cgccccaaat
     3901 ccagtggagg cacacaggtg gaagatgccg taccagctcg atgagctgct caatttccat
     3961 atcccatact acgtcggccc tctgattaca ccgaagcagc aggcggagtc aggcgagaac
     4021 gtgttcgcct ggatggtcag gaaggacccg tctggcaaca tcaccccata caatttcgat
     4081 gagaaggtgg accgggaggc gtcagccaat acattcatcc agaggatgaa gaccacagat
     4141 acctacctga ttggcgagga cgtgctccct aagcagtctc tgctctacca gaagtacgag
     4201 gtgctcaacg agctgaacaa tgtccgcatc aacaatgagt gcctgggcac agatcagaag
     4261 cagcggctca tcagggaggt gttcgagcgg cactcatctg tgaccattaa gcaggtcgcg
     4321 gataatctcg tggcacatgg cgacttcgcc cggaggccgg agatcagggg cctggcggac
     4381 gagaagaggt tcctctccag cctgtccaca taccaccagc tcaaggagat cctgcatgag
     4441 gccattgacg atccaaccaa gctgctcgat atcgagaaca tcattacctg gagcacagtg
     4501 ttcgaggacc acaccatctt cgagacaaag ctggccgaga ttgagtggct cgacccgaag
     4561 aagatcaacg agctgtcagg cattcgctac cggggatggg gacagttctc tcgcaagctg
     4621 ctcgacggcc tcaagctggg caacggccac acagtcattc aggagctgat gctgagcaac
     4681 cataatctca tgcagatcct ggccgatgag acactcaagg agacaatgac agagctgaac
     4741 caggacaagc tcaagacaga cgatatcgag gatgtgatta acgacgcgta cacctcacca
     4801 tctaataaga aggccctccg gcaggtcctg agggtggtcg aggatattaa gcatgccgcg
     4861 aatggccagg acccttcatg gctcttcatc gagacagccg acggcaccgg cacagccggc
     4921 aagcgcacac agtctcggca gaagcagatc cagaccgtgt acgcgaacgc cgcgcaggag
     4981 ctgattgatt ccgccgtgag gggcgagctg gaggataaga tcgcggacaa ggcctctttc
     5041 acagaccgcc tcgtgctgta cttcatgcag ggcggcaggg atatctacac aggcgcccct
     5101 ctgaatattg accagctctc ccactacgac atcgatcata ttctcccgca gtcactgatt
     5161 aaggacgatt ctctggacaa cagggtgctc gtcaacgcga caatcaatcg cgagaagaac
     5221 aatgtgttcg cctccacact cttcgcgggc aagatgaagg caacctggcg gaagtggcat
     5281 gaggcaggcc tgatttcagg acgcaagctc cggaatctca tgctgaggcc ggatgagatc
     5341 gacaagttcg ccaagggatt cgtggcaagg cagctggtgg agacaaggca gatcattaag
     5401 ctcacagagc agatcgctgc cgcccagtac cctaacacca agatcattgc cgtgaaggcg
     5461 ggcctcagcc accagctgag ggaggagctg gatttcccga agaacaggga cgtgaatcat
     5521 taccaccatg cgttcgatgc cttcctcgcc gcaaggattg gcacatacct gctcaagcgc
     5581 tacccgaagc tggcgccttt cttcacctac ggcgagttcg ccaaggtgga cgtgaagaag
     5641 ttcagggagt tcaacttcat cggcgcgctc acacacgcca agaagaatat cattgcgaag
     5701 gataccggcg agattgtgtg ggataaggag agggacatcc gcgagctgga caggatctac
     5761 aatttcaagc gcatgctcat tacccatgag gtctacttcg agacagccga tctcttcaag
     5821 cagaccatct acgccgcaaa ggactccaag gagaggggcg gcagcaagca gctgattcct
     5881 aagaagcagg gctacccgac acaggtgtac ggcggataca cccaggagtc aggcagctac
     5941 aatgcgctcg tgcgcgtggc cgaggcagat accacagcct accaggtcat caagatttcc
     6001 gcgcagaacg cctccaagat cgcgagcgcc aatctcaaga gcagggagaa gggcaagcag
     6061 ctgctcaatg agattgtggt caagcagctg gcgaagcgcc ggaagaactg gaagccttca
     6121 gccaattctt tcaagatcgt gattccgagg ttcggcatgg gcacactgtt ccagaacgcc
     6181 aagtacggcc tcttcatggt caactcagac acctactacc gcaattacca ggagctgtgg
     6241 ctgtcccggg agaatcagaa gctgctcaag aagctcttca gcatcaagta cgagaagaca
     6301 cagatgaacc acgatgccct ccaggtgtac aaggccatca ttgaccaggt cgagaagttc
     6361 ttcaagctgt acgatatcaa tcagttcagg gcgaagctct ccgacgccat tgagcgcttc
     6421 gagaagctgc cgatcaacac cgatggcaat aagattggca agaccgagac actccggcag
     6481 atcctcattg gcctccaggc gaacggcaca aggtccaacg tgaagaatct gggcatcaag
     6541 accgacctcg gcctgctcca ggtcggcagc ggcatcaagc tcgacaagga tacccagatt
     6601 gtctaccagt caccgtctgg cctgttcaag aggcgcatcc cactcgccga cctgtcaagg
     6661 gctgatccta agaagaagag gaaggtttct agggcagatc caaagaagaa gcggaaggtg
     6721 tctagagctg atccgaagaa gaagcgcaag gtgtgactcg agatatgaag atgaagatga
     6781 aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt
     6841 gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc acattataat
     6901 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat
     6961 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata agaggatccg
     7021 cggatcatga accaacggcc tggctgtatt tggtggttgt gtagggagat ggggagaaga
     7081 aaagcccgat tctcttcgct gtgatgggct ggatgcatgc gggggagcgg gaggcccaag
     7141 tacgtgcacg gtgagcggcc cacagggcga gtgtgagcgc gagaggcggg aggaacagtt
     7201 tagtaccaca ttgcccagct aactcgaacg cgaccaactt ataaacccgc gcgctgtcgc
     7261 ttgtgtggag accttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
     7321 cgcggtggct gagatcagcc acttcttccc cgataacgga aaccggcaca ctggccatat
     7381 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
     7441 agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
     7501 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
     7561 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt
     7621 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
     7681 cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca
     7741 tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca
     7801 tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca
     7861 aaaatcagcg cgcaaatacg catactgtta tctggcttgg taccggtctc agtctcaggt
     7921 agatgtcaga tcaatcagaa atgattgatc tgacatctac gagttgagat caaacaaagc
     7981 ttcagctgag tttcaatttc tgagcccatg ttgggccata catatgccac ccgagtgcaa
     8041 atcgggtggc tttttttcta gacccagctt tcttgtacaa agttggcatt actgcgagct
     8101 caaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     8161 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     8221 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     8281 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     8341 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     8401 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     8461 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     8521 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     8581 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     8641 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     8701 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     8761 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     8821 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     8881 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     8941 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     9001 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     9061 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     9121 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     9181 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     9241 ggctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     9301 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     9361 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     9421 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     9481 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     9541 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     9601 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     9661 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     9721 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     9781 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     9841 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     9901 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     9961 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
    10021 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
    10081 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
    10141 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
    10201 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
    10261 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
    10321 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
    10381 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
    10441 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
    10501 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
    10561 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
    10621 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
    10681 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
    10741 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
    10801 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
    10861 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
    10921 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
    10981 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
    11041 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
    11101 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
    11161 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
    11221 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
    11281 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
    11341 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
    11401 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
    11461 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
    11521 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
    11581 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt
    11641 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
    11701 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
    11761 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
    11821 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
    11881 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
    11941 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
    12001 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
    12061 gtgaaaacct ctgacacatg cagctcccgg aaacggtcac agcttgtctg taagcggatg
    12121 ccgggagcag acaagcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    12181 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
    12241 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    12301 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    12361 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    12421 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    12481 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    12541 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    12601 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    12661 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    12721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    12781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    12841 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12901 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    12961 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    13021 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    13081 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    13141 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
    13201 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgtc atactgttct
    13261 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
    13321 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
    13381 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
    13441 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
    13501 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
    13561 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
    13621 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
    13681 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
    13741 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
    13801 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
    13861 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
    13921 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
    13981 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
    14041 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
    14101 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
    14161 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
    14221 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
    14281 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
    14341 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
    14401 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
    14461 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
    14521 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
    14581 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
    14641 ctcacacatt attatggaga aaatagagct tgtcgatcgt taattaatca cctccaagat
    14701 cccggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
    14761 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
    14821 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
    14881 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
    14941 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
    15001 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
    15061 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
    15121 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
    15181 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
    15241 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
    15301 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
    15361 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
    15421 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca
    15481 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
    15541 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
    15601 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggacac gctgtcgaac
    15661 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcctagga
    15721 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
    15781 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
    15841 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
    15901 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
    15961 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
    16021 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
    16081 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
    16141 tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa
    16201 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    16261 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    16321 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    16381 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    16441 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
//
