LOCUS       sequence-388291-       17498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 cytosine base editing plasmid in wheat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17498)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 17498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL671
FEATURES             Location/Qualifiers
     source          1..17498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6131..6151
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6359..6424
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7028..7048
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7055..7306
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7307..7360
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7547..7567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7580..7600
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7613..7633
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7644..7892
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8272..8577)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8374..8393)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9060..9084)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9114..9138
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10439..11068
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11497..12570
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12636..12830
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13066..13088)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13164..13304
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13219..13238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13319..13336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13490..14078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13570..13589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14165..14959)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15384..15408
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    15486..15660
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(16750..17426)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(16793..16813)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tgacaaagct
     2041 gaaccagcca tacggcatcg gcctcgctat tggctcaaac tctatcggct tcgcggtggt
     2101 cgacgccaat tcccacctcc tgcgcctgaa gggcgagaca gccatcggcg ccaggctctt
     2161 ccgcgaggga cagtcagccg cggatcggag gggctctagg accacacgga ggcgcctctc
     2221 ccggacaagg tggcgcctca gcttcctgcg cgacttcttc gcgccacata tcaccaagat
     2281 tgaccctgat ttcttcctcc gccagaagta ctccgagatc agccctaagg acaaggatcg
     2341 gttcaagtac gagaagaggc tgttcaacga ccgcaccgat gccgagttct acgaggatta
     2401 cccatcaatg taccacctga ggctccatct gatgacccac acacataagg ccgacccgag
     2461 ggagattttc ctcgccatcc accatattct caagtctcgg ggccacttcc tcacacctgg
     2521 agcggccaag gatttcaata ccgacaaggt ggatctggag gacatcttcc cagccctcac
     2581 agaggcgtac gcccaggtct acccggacct ggagctgacc ttcgacctcg cgaaggccga
     2641 cgatttcaag gcgaagctcc tggatgagca ggccaccccg tccgacacac agaaggcgct
     2701 ggtgaacctc ctgctctcca gcgatggcga gaaggagatt gtcaagaaga ggaagcaggt
     2761 gctgacagag ttcgcgaagg ccatcaccgg cctcaagaca aagttcaacc tcgccctggg
     2821 caccgaggtg gatgaggccg atgcctcaaa ttggcagttc tctatgggcc agctcgacga
     2881 taagtggtcc aacatcgaga catccatgac agaccagggc accgagattt tcgagcagat
     2941 tcaggagctg taccgggcga ggctgctcaa cggcattgtg ccagccggca tgtccctgag
     3001 ccaggccaag gtcgccgatt acggccagca caaggaggac ctggagctgt tcaagacata
     3061 cctcaagaag ctgaatgatc atgagctggc gaagaccatc cggggcctct acgacaggta
     3121 cattaacggc gacgatgcca agccgttcct gcgcgaggac ttcgtcaagg cgctcaccaa
     3181 ggaggtgaca gcccatccaa acgaggtgtc cgagcagctg ctcaacagga tgggccaggc
     3241 caatttcatg ctcaagcaga ggaccaaggc caacggcgca atcccaattc agctccagca
     3301 gcgcgagctg gatcagatca ttgcgaacca gagcaagtac tacgactggc tcgccgcccc
     3361 aaatccagtg gaggcacaca ggtggaagat gccgtaccag ctcgatgagc tgctcaattt
     3421 ccatatccca tactacgtcg gccctctgat tacaccgaag cagcaggcgg agtcaggcga
     3481 gaacgtgttc gcctggatgg tcaggaagga cccgtctggc aacatcaccc catacaattt
     3541 cgatgagaag gtggaccggg aggcgtcagc caatacattc atccagagga tgaagaccac
     3601 agatacctac ctgattggcg aggacgtgct ccctaagcag tctctgctct accagaagta
     3661 cgaggtgctc aacgagctga acaatgtccg catcaacaat gagtgcctgg gcacagatca
     3721 gaagcagcgg ctcatcaggg aggtgttcga gcggcactca tctgtgacca ttaagcaggt
     3781 cgcggataat ctcgtggcac atggcgactt cgcccggagg ccggagatca ggggcctggc
     3841 ggacgagaag aggttcctct ccagcctgtc cacataccac cagctcaagg agatcctgca
     3901 tgaggccatt gacgatccaa ccaagctgct cgatatcgag aacatcatta cctggagcac
     3961 agtgttcgag gaccacacca tcttcgagac aaagctggcc gagattgagt ggctcgaccc
     4021 gaagaagatc aacgagctgt caggcattcg ctaccgggga tggggacagt tctctcgcaa
     4081 gctgctcgac ggcctcaagc tgggcaacgg ccacacagtc attcaggagc tgatgctgag
     4141 caaccataat ctcatgcaga tcctggccga tgagacactc aaggagacaa tgacagagct
     4201 gaaccaggac aagctcaaga cagacgatat cgaggatgtg attaacgacg cgtacacctc
     4261 accatctaat aagaaggccc tccggcaggt cctgagggtg gtcgaggata ttaagcatgc
     4321 cgcgaatggc caggaccctt catggctctt catcgagaca gccgacggca ccggcacagc
     4381 cggcaagcgc acacagtctc ggcagaagca gatccagacc gtgtacgcga acgccgcgca
     4441 ggagctgatt gattccgccg tgaggggcga gctggaggat aagatcgcgg acaaggcctc
     4501 tttcacagac cgcctcgtgc tgtacttcat gcagggcggc agggatatct acacaggcgc
     4561 ccctctgaat attgaccagc tctcccacta cgacatcgat catattctcc cgcagtcact
     4621 gattaaggac gattctctgg acaacagggt gctcgtcaac gcgacaatca atcgcgagaa
     4681 gaacaatgtg ttcgcctcca cactcttcgc gggcaagatg aaggcaacct ggcggaagtg
     4741 gcatgaggca ggcctgattt caggacgcaa gctccggaat ctcatgctga ggccggatga
     4801 gatcgacaag ttcgccaagg gattcgtggc aaggcagctg gtggagacaa ggcagatcat
     4861 taagctcaca gagcagatcg ctgccgccca gtaccctaac accaagatca ttgccgtgaa
     4921 ggcgggcctc agccaccagc tgagggagga gctggatttc ccgaagaaca gggacgtgaa
     4981 tcattaccac catgcgttcg atgccttcct cgccgcaagg attggcacat acctgctcaa
     5041 gcgctacccg aagctggcgc ctttcttcac ctacggcgag ttcgccaagg tggacgtgaa
     5101 gaagttcagg gagttcaact tcatcggcgc gctcacacac gccaagaaga atatcattgc
     5161 gaaggatacc ggcgagattg tgtgggataa ggagagggac atccgcgagc tggacaggat
     5221 ctacaatttc aagcgcatgc tcattaccca tgaggtctac ttcgagacag ccgatctctt
     5281 caagcagacc atctacgccg caaaggactc caaggagagg ggcggcagca agcagctgat
     5341 tcctaagaag cagggctacc cgacacaggt gtacggcgga tacacccagg agtcaggcag
     5401 ctacaatgcg ctcgtgcgcg tggccgaggc agataccaca gcctaccagg tcatcaagat
     5461 ttccgcgcag aacgcctcca agatcgcgag cgccaatctc aagagcaggg agaagggcaa
     5521 gcagctgctc aatgagattg tggtcaagca gctggcgaag cgccggaaga actggaagcc
     5581 ttcagccaat tctttcaaga tcgtgattcc gaggttcggc atgggcacac tgttccagaa
     5641 cgccaagtac ggcctcttca tggtcaactc agacacctac taccgcaatt accaggagct
     5701 gtggctgtcc cgggagaatc agaagctgct caagaagctc ttcagcatca agtacgagaa
     5761 gacacagatg aaccacgatg ccctccaggt gtacaaggcc atcattgacc aggtcgagaa
     5821 gttcttcaag ctgtacgata tcaatcagtt cagggcgaag ctctccgacg ccattgagcg
     5881 cttcgagaag ctgccgatca acaccgatgg caataagatt ggcaagaccg agacactccg
     5941 gcagatcctc attggcctcc aggcgaacgg cacaaggtcc aacgtgaaga atctgggcat
     6001 caagaccgac ctcggcctgc tccaggtcgg cagcggcatc aagctcgaca aggataccca
     6061 gattgtctac cagtcaccgt ctggcctgtt caagaggcgc atcccactcg ccgacctgtc
     6121 aagggctgat cctaagaaga agaggaaggt tggaggtggc ggtacaggag ggggtgggtc
     6181 cgctgagtat gtcagggcgt tgttcgactt caatggaaac gacgaggaag atctgccttt
     6241 taaaaaggga gatattctca ggatcagaga taagccggaa gaacaatggt ggaacgctga
     6301 agactctgaa ggtaagagag gtatgattct tgtcccctac gtcgagaagt attcgggtga
     6361 ctataaagac cacgatggag attataagga ccacgatata gattataagg atgatgatga
     6421 taagagcgga atgaccgatg cagagtacgt caggattcat gagaaacttg acatctacac
     6481 gtttaagaaa cagtttttca acaacaaaaa atctgttagt caccgctgtt acgtgctgtt
     6541 cgaattgaaa cgcagaggtg agaggagagc ctgcttttgg ggctatgccg tcaacaagcc
     6601 gcaaagcggc acagaaaggg gcattcacgc ggagatattt agcattagaa aggtcgagga
     6661 ataccttcgg gataatcccg ggcaattcac tatcaattgg tactcttcat ggtccccgtg
     6721 tgcagattgc gctgaaaaga tactggagtg gtataatcaa gaactcagag gaaacggtca
     6781 caccctcaag atttgggctt gcaagcttta ctacgagaaa aatgcaagga accagatcgg
     6841 cctctggaac ttgcgcgaca acggcgtggg gttgaatgtg atggtgtcgg agcattacca
     6901 gtgctgccgg aagatattca ttcagtcgtc acataatcaa ttgaacgaga ataggtggct
     6961 cgaaaaaacc ctgaagcggg ccgagaagtg gaggagtgaa ctctcgataa tgatccaggt
     7021 taaaggaccg aagaagaagc gcaaggtggg gaccatgact aatctctcag atataatcga
     7081 gaaggaaaca ggaaagcaac tggtcatcca agaatcgatt ttgatgcttc ccgaagaagt
     7141 cgaagaagtt ataggaaata agcccgagtc tgacatactg gttcacacag cgtacgatga
     7201 aagtacggac gagaatgtca tgttgctgac atcggacgca cctgaataca agccttgggc
     7261 tctggtcata caagatagta acggagaaaa taagattaaa atgcttgaag gaaggggctc
     7321 cctgctgaca tgtggagatg tggaagagaa tccaggacca tccaggatga tagagcaaga
     7381 tggttgtacc caagtgctgc ggcccctcgg atggcggggg tacagcgcca tgactggcca
     7441 taatagacag tccgctgctc tcatgcctcc atgcagcgga tgtcagagga ggggagctag
     7501 gttcttcctg tctagaccta cctgccccgt gccctcaagg gctgatccta agaagaagag
     7561 gaaggtttct agggcagatc caaagaagaa gcggaaggtg tctagagctg atccgaagaa
     7621 gaagcgcaag gtgtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata
     7681 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     7741 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat
     7801 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     7861 ggtatggact atggaatatg attaaagata agaggatccg cgaccaagcc cgttattctg
     7921 acagttctgg tgctcaacac atttatattt atcaaggagc acattgttac tcactgctag
     7981 gagggaatcg aactaggaat attgatcaga ggaactacga gagagctgaa gataactgcc
     8041 ctctagctct cactgatctg ggtcgcatag tgagatgcag cccacgtgag ttcagcaacg
     8101 gtctagcgct gggcttttag gcccgcatga tcgggctttt gtcgggtggt cgacgtgttc
     8161 acgattgggg agagcaacgc agcagttcct cttagtttag tcccacctcg cctgtccagc
     8221 agagttctga ccggtttata aactcgcttg ctgcatcaga cttgggagac cttatattcc
     8281 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     8341 ttcttccccg ataacggaaa ccggcacact ggccatatcg gtggtcatca tgcgccagct
     8401 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
     8461 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
     8521 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
     8581 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
     8641 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
     8701 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
     8761 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata
     8821 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
     8881 tactgttatc tggcttggta ccggtctcag tctcaggtag atgtcagatc aatcagaaat
     8941 gattgatctg acatctacga gttgagatca aacaaagctt cagctgagtt tcaatttctg
     9001 agcccatgtt gggccataca tatgccaccc gagtgcaaat cgggtggctt tttttctaga
     9061 cccagctttc ttgtacaaag ttggcattac tgcgagctca aactatcagt gtttgacagg
     9121 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     9181 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     9241 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     9301 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     9361 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     9421 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     9481 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     9541 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     9601 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     9661 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     9721 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     9781 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     9841 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     9901 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     9961 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
    10021 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
    10081 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
    10141 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
    10201 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg
    10261 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
    10321 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
    10381 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
    10441 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
    10501 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
    10561 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
    10621 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
    10681 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
    10741 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
    10801 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
    10861 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
    10921 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
    10981 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
    11041 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
    11101 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
    11161 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
    11221 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
    11281 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
    11341 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
    11401 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
    11461 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
    11521 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
    11581 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
    11641 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
    11701 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
    11761 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
    11821 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
    11881 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
    11941 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
    12001 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
    12061 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
    12121 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
    12181 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
    12241 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
    12301 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
    12361 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
    12421 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
    12481 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
    12541 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
    12601 ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac
    12661 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
    12721 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
    12781 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
    12841 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
    12901 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
    12961 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
    13021 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    13081 gctcccggaa acggtcacag cttgtctgta agcggatgcc gggagcagac aagcgcgtca
    13141 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    13201 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    13261 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    13321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    13381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    13441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    13501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    13561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    13621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    13681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    13741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    13801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13921 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    13981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    14041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    14101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    14161 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    14221 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    14281 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    14341 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    14401 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    14461 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    14521 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    14581 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    14641 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    14701 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    14761 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    14821 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    14881 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    14941 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    15001 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    15061 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    15121 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    15181 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    15241 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    15301 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    15361 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    15421 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
    15481 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
    15541 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
    15601 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
    15661 atagagcttg tcgatcgtta attaatcacc tccaagatcc cggacgagtg ctggggcgtc
    15721 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
    15781 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
    15841 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
    15901 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
    15961 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
    16021 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
    16081 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
    16141 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
    16201 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
    16261 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
    16321 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
    16381 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
    16441 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
    16501 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
    16561 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
    16621 cctccgagag ctgcatcagg tcggacacgc tgtcgaactt ttcgatcaga aacttctcga
    16681 cagacgtcgc ggtgagttca ggctttttca tcctaggaga gatagatttg tagagagaga
    16741 ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
    16801 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
    16861 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
    16921 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
    16981 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
    17041 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
    17101 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
    17161 gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
    17221 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
    17281 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
    17341 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
    17401 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
    17461 tattcttgga gtagacgaga gtgtcgtgct ccaccatg
//
