LOCUS       sequence-388306-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Y to S mutation in VPS13D gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Guillen-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli
            P
  TITLE     VPS13D bridges the ER to mitochondria and peroxisomes via Miro.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212021. doi: 
            10.1083/jcb.202010004.
  PUBMED    33891013
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212021. doi"; 
            pages: " 10.1083/jcb.202010004"
COMMENT     Sequence Label: VPS13D1-1576-(Y1253S)
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        159..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5447..5456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5453..5455,5456..5458,5459..6172)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5453..5455,5456..5458,5459..6169)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5498..5519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5759..5778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6106..6127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6295..6416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6332..6351)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6386..6405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6423..6878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6560..6581)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6772..6791
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6905..7009
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7011..7368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7011..7202
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7034..7054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7219..7354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7403..8197
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7403..8197
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7457..7476)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8067..8086
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8385..8404)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8429..8476
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8805..9393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9294..9313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
       61 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctggtt tagtgaaccg tcagatccgc caccatgctg gagggcctgg tcgcttgggt
      721 cctgaacacc tacctgggga agtatgtgaa caacctgaac accgatcagc tgtccgtcgc
      781 tctgctgaag ggcgccgtgg agctggagaa cctgccactg aagaaggatg ccctgaagga
      841 gctggagctg cccttcgagg tgaaggccgg ctttatcggc aaggtgaccc tgcagatccc
      901 tttttacagg ccacacgtgg acccctgggt catcagcatc agctccctgc acctgatcgg
      961 cgcccctgag aagatccagg acttcaatga tgagaaggag aagctgctgg agagagagag
     1021 gaagaaggcc ctgctgcagg ccctggagga gaagtggaag aacgaccgcc agcagaaggg
     1081 cgagtcctac tggtattctg tgaccgccag cgtggtgaca agaatcgtgg agaatatcga
     1141 gctgaagatc caggacgtgc acctgaggtt cgaggatggc gtgaccaacc cttctcaccc
     1201 attcgccttt ggcatctgca tcaagaatgt gagcatgcag aatgccgtga acgagcccgt
     1261 gcagaagctg atgcggaaga agcagctgga tgtggccgag ttttccatct actgggacgt
     1321 ggattgtaca ctgctgggcg acctgcctca gatggagctg caggaggcaa tggcacgcag
     1381 catggagagc cggtcccacc actatgtgct ggagccagtg ttcgccagcg ccctgctgaa
     1441 gcgcaactgc tccaagaagc ctctgcgctc tcggcacagc ccacggatcg actgtgatat
     1501 ccagctggag acaatccccc tgaagctgtc ccagctgcag tacagacaga tcatggagtt
     1561 tctgaaggag ctggagcgca aggagcggca ggtgaagttc cggagatgga agcctaaggt
     1621 ggccatcagc aagaactgcc gcgagtggtg gtatttcgcc ctgaacgcca atctgtatga
     1681 gatccgggag cagagaaaga ggtgtacctg ggactttatg ctgcacaggg caagggacgc
     1741 cgtgtcttac accgataagt atttcaataa gctgaagggc ggcctgctga gcacagacga
     1801 taaggaggag atgtgccgga tcgaggagga gcagtccttt gaggagctga agatcctgcg
     1861 cgagctggtg cacgatcggt tccacaagca ggaggagctg gccgagtctc tgagagagcc
     1921 ccagtttgac agccctggcg cctgtccagg agcaccagag cctggaggag gctccggaat
     1981 gctgcagtac ctgcagtctt ggttccctgg atggggagga tggtatggac agcagacccc
     2041 agagggaaac gtggtggagg gcctgtccgc cgagcagcag gagcagtgga ttcccgagga
     2101 gatcctgggc accgaggagg gcggtgaccc aacagccgat gccagctgca tgaacacata
     2161 caccaagcgc gaccacgtgt tcgccaagct gaatctgcag ctgcagcggg gcacagtgac
     2221 cctgctgcac aaggagcagg gaaccccaca gatgaacgag agcgccttca tgcagctgga
     2281 gttttccgat gtgaagctgc tggccgagtc tctgcctagg cgcaattcta gcctgctgag
     2341 cgtgcgcctg ggaggcctgt ttctgcggga cctggcaaca gagggcacca tgttccccct
     2401 gctggtgttt ccaaaccccc agaaggaagt gggcagagtg tctcagagct tcggcctgca
     2461 gaccacatcc gccgacaggt ctgatcacta cccagcagcc gaccccgatg gccccgtgtt
     2521 cgagatgctg tatgagagaa atcccgccca cagccacttc gagcggagac tgaacgtgtc
     2581 caccaggccc ctgaatatca tctacaaccc tcaggccatc aagaaggtgg ccgacttctt
     2641 ttacaagggc aaggtgcaca catctggctt cggctatcag agcgagctgg agctgagagt
     2701 ggcagaggca gcaaggcgcc agtataacaa gctgaagatg cagaccaagg ccgagatcag
     2761 acagacactg gacaggctgc tggtgggcga cttcatcgag gagtctaagc ggtggaccgt
     2821 gcggctggat atcagcgccc cacaggtcat cttccccgac gatttcaagt ttaagaatcc
     2881 tgtgctggtg gtggtggacc tgggcagaat gctgctgaca aatacccagg ataacagccg
     2941 gagaaagtcc agggacggct ccgcctctga ggagacacag ttctccgacg atgagtacaa
     3001 gacaccactg gccacccccc ctaatacacc accccctgag tcctctagct ccaacggcga
     3061 gaagacccca cccttctccg gcgtggagtt ttctgaggag cagctgcagg cccacctgat
     3121 gagcacaaag atgtacgagc ggtacagcct gtcctttatg gatctgcaga tcatggtggg
     3181 ccgggtgaag gacaactgga agcacgtgca ggacatcgat gtgggcccca cccacgtggt
     3241 ggagaagttc aatgtgcacc tgcagctgga gaggcgcctg atctacacat ccgacccaaa
     3301 gtatccagga gccgtgctgt ctggaaacct gcctgatctg aagatccaca tcaatgagga
     3361 caagatcagc gccctgaaga actgctttgc cctgctgacc acaccagaga tgaagacatc
     3421 cgatacccag atcaaggaga agatcttccc acaggaggag cagaggggct ctctgcagga
     3481 cagcgtgatg aacctgaccc agtctatcgt gctgctggag cagcacacaa gagaggtgct
     3541 ggtggagagc cagctgctgc tggccgagtt caaggtcaat tgtatgcagc tgggcgtgga
     3601 gtccaacggc agatacatct ctgtgctgaa ggtgttcggc accaacgccc actttgtgaa
     3661 gaggccatat gatgccgagg tgagcctgac agtgcacggc ctgctgctgg tggacacaat
     3721 gcagacctac ggcgccgact ttgatctgct gatggccagc cacaagaatc tgtccttcga
     3781 tatccccacc ggctccctga gagactctag ggcacagtcc cccgtgtctg gacctaatgt
     3841 ggcacacctg accgacggag ccacactgaa cgataggtct gccacctctg tgagcctgga
     3901 caagatcctg acaaaggagc aggagagcct gatcaagctg gagtatcagt tcgtgtctag
     3961 cgagtgcccc tctatgaacc tggatagcac cctgcaggtc atcagcctgc aggtgaacaa
     4021 tctggacatc atcctgaatc ctgagacaat cgtggagctg atcggcttcc tgcagaagtc
     4081 ttttccaaag gagaaggacg atctgagccc tcagccactg atgaccgatt tcgagcggag
     4141 cttccgggag cagggcacct accagtctac atatgagcag aataccgagg tggccgtgga
     4201 gatccacaga ctgaacctgc tgctgctgag gacagtgggc atggccaatc gcgagaagta
     4261 cggaaggaag atcgcaaccg caagcatcgg cggcacaaaa gtgaatgtga gcatgggctc
     4321 cacctttgac atgaacggct ccctgggctg cctgcagctg atggacctga cacaggataa
     4381 tgtgaagaac cagtatgtgg tgagcatcgg caattccgtg ggctacgaga acatcatctc
     4441 tgatatcggc tctttcgaga gcgtgtttgt gcgcatggag gacgccgccc tgaccgaggc
     4501 cctgtccttc acatttgtgg agcggtctaa gcaggagtgt ttcctgaatc tgaagatggc
     4561 cagcctgcac tacaaccact ccgccaagtt tctgaaggag ctgaccctgt ctatggacga
     4621 gctggaggag aacttcaggg gcatgctgaa gagcgccgcc acaaaggtga ccacagtgct
     4681 ggccacaaag accgccgagt actctgagat ggtgagcctg ttcgagacac ctagaaagac
     4741 aagggagccc ttcatcctgg aggagaatga gatctatggc ttcgacctgg cctcctctca
     4801 cctggatacc gtgaagctga tcctgaacat caatatcgag tcccccgtgg tgtctatccc
     4861 taggaagcca ggctcccccg agctgctggt gggacacctg ggacagatct tcatccagaa
     4921 ctttgtggcc ggcgacgatg agtcccgctc tgatcggctg caggtggaga tcaaggacat
     4981 caagctgtac agcctgaatt gcacccagct ggcaggaaga gaggcagtgg gctctgaggg
     5041 cagcaggatg ttttgtcctc caagcggctc cggctctgcc aactcccagg aggaggcaca
     5101 cttcacacgc cacgacggct ttgagtctct gcaccggggc caggcctttc acatcctgaa
     5161 caataccaca atccagttca agctggagaa gatcccaatc gagcgggaga gcgagctgac
     5221 ctttagcctg tcccccgacg atctgggcac aagctccatc atgaagatcg agggcaagtt
     5281 cgtgaatcca gtgcaggtgg tgctggccaa gcacgtgtac gagcaggtgc tgcagaccct
     5341 ggacaacctg gtgtacagcg aggatctgaa taagtatcca gcatccgcca catctagccc
     5401 atgccctgat tccccactgc caggctctgg ctcctctcca ccggtcgcca ccatggtgag
     5461 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5521 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5581 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5641 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     5701 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5761 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5821 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5881 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5941 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6001 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6061 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6121 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     6181 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     6241 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6301 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6361 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     6421 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6481 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6541 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6601 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6661 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6721 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6781 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6841 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     6901 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6961 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     7021 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     7381 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     7441 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7501 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7561 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     7621 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7681 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7741 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7801 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     7861 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7921 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7981 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8041 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8101 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8161 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     8221 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     8281 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8341 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     8401 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     8461 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     8521 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     8581 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     8641 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgg
//
