LOCUS       sequence-388328-        8782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector to express human PC with MYC tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, 
            Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, 
            Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong
            N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner 
            L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, 
            Ferbeyre G
  TITLE     A hydride transfer complex reprograms NAD metabolism and bypasses 
            senescence.
  JOURNAL   Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 
            10.1016/j.molcel.2021.08.028.
  PUBMED    34547241
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.08"; journalName: "Mol Cell"; date: 
            "2021-09-16- 16"; volume: "81"; issue: "18"; pages: "3848-3865.e19"
COMMENT     Sequence Label: pBABE-neomycin-PC-MYC
FEATURES             Location/Qualifiers
     source          1..8782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4908..4937
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4970..4990)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4975..5304
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5155..5290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5314..6108
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5368..5387)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5978..5997
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6276..6745
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6875..6892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7046..7634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7126..7145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7794..8585,8586..8654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8417..8436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8655..8759)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcccc accatgctga
     1381 agttccgaac agtccatggg ggcctgaggc tcctgggaat ccgccgaacc tccaccgccc
     1441 ccgctgcctc cccaaatgtc cggcgcctgg agtataagcc catcaagaaa gtcatggtgg
     1501 ccaacagagg tgagattgcc atccgtgtgt tccgggcctg cacggagctg ggcatccgca
     1561 ccgtagccat ctactctgag caggacacgg gccagatgca ccggcagaaa gcagatgaag
     1621 cctatctcat cggccgcggc ctggcccccg tgcaggccta cctgcacatc ccagacatca
     1681 tcaaggtggc caaggagaac aacgtagatg cagtgcaccc tggctacggg ttcctctctg
     1741 agcgagcgga cttcgcccag gcctgccagg atgcaggggt ccggtttatt gggccaagcc
     1801 cagaagtggt ccgcaagatg ggagacaagg tggaggcccg ggccatcgcc attgctgcgg
     1861 gtgttcccgt tgtccctggc acagatgccc ccatcacgtc cctgcatgag gcccacgagt
     1921 tctccaacac ctacggcttc cccatcatct tcaaggcggc ctatgggggt ggagggcgtg
     1981 gcatgagggt ggtgcacagc tacgaggagc tggaggagaa ttacacccgg gcctactcag
     2041 aggctctggc cgcctttggg aatggggcgc tgtttgtgga gaagttcatc gagaagccac
     2101 ggcacatcga ggtgcagatc ttgggggacc agtatgggaa catcctgcac ctgtacgagc
     2161 gagactgctc catccagcgg cggcaccaga aggtggtcga gattgccccc gccgcccacc
     2221 tggacccgca gcttcggact cggctcacca gcgactctgt gaaactcgct aaacaggtgg
     2281 gctacgagaa cgcaggcacc gtggagttcc tggtggacag gcacggcaag cactacttca
     2341 tcgaggtcaa ctcccgcctg caggtggagc acacggtcac agaggagatc accgacgtag
     2401 acctggtcca tgctcagatc cacgtggctg agggcaggag cctacccgac ctgggcctgc
     2461 ggcaggagaa catccgcatc aacgggtgtg ccatccagtg ccgggtcacc accgaggacc
     2521 ccgcgcgcag cttccagccg gacaccggcc gcattgaggt gttccggagc ggagagggca
     2581 tgggcatccg cctggataat gcttccgcct tccaaggagc cgtcatctcg ccccactacg
     2641 actccctgct ggtcaaagtc attgcccacg gcaaagacca ccccacggcc gccaccaaga
     2701 tgagcagggc ccttgcggag ttccgcgtcc gaggtgtgaa gaccaacatc gccttcctgc
     2761 agaatgtgct caacaaccag cagttcctgg caggcactgt ggacacccag ttcatcgacg
     2821 agaacccaga gctgttccag ctgcggcctg cacagaaccg ggcccaaaag ctgttgcact
     2881 acctcggcca tgtcatggta aacggtccaa ccaccccgat tcccgtcaag gccagcccca
     2941 gccccacgga ccccgttgtc cctgcagtgc ccataggccc gcccccggct ggtttcagag
     3001 acatcctgct gcgagagggg cctgagggct ttgctcgagc tgtgcggaac cacccggggc
     3061 tgctgctgat ggacacgacc ttcagggacg cccaccagtc actgctggcc actcgtgtgc
     3121 gcacccacga tctcaaaaag atcgccccct atgttgccca caacttcagc aagctcttca
     3181 gcatggagaa ctggggagga gccacgtttg acgtcgccat gcgcttcctg tatgagtgcc
     3241 cctggcggcg gctgcaggag ctccgggagc tcatccccaa catccctttc cagatgctgc
     3301 tgcggggggc caatgctgtg ggctacacca actacccaga caacgtggtc ttcaagttct
     3361 gtgaagtggc caaagagaat ggcatggatg tcttccgtgt gtttgactcc ctcaactact
     3421 tgcccaacat gctgctgggc atggaggcgg caggaagtgc cggaggcgtg gtggaggctg
     3481 ccatctcata cacgggcgac gtggccgacc ccagccgcac caagtactca ctgcagtact
     3541 acatgggctt ggccgaagag ctggtgcgag ctggcaccca catcctgtgc atcaaggaca
     3601 tggccgggct gctgaagccc acggcctgca ccatgctggt cagctccctc cgggaccgct
     3661 tccccgacct cccactgcac atccacaccc acgacacgtc aggggcaggc gtggcagcca
     3721 tgctggcctg tgcccaggct ggagctgatg tggtggatgt ggcagctgat tccatgtctg
     3781 ggatgacttc acagcccagc atgggggccc tggtggcctg taccagaggg actcccctgg
     3841 acacagaggt gcccatggag cgcgtgtttg actacagtga gtactgggag ggggctcggg
     3901 gactgtacgc ggccttcgac tgcacggcca ccatgaagtc tggcaactcg gacgtgtatg
     3961 aaaatgagat cccagggggc cagtacacca acctgcactt ccaggcccac agcatggggc
     4021 ttggctccaa gttcaaggag gtcaagaagg cctatgtgga ggccaaccag atgctgggcg
     4081 atctcatcaa ggtgacgccc tcctccaaga tcgtggggga cctggcccag tttatggtgc
     4141 agaatggatt gagccgggca gaggccgaag ctcaggcgga agagctgtcc tttccccgct
     4201 ccgtggtgga gttcctgcag ggctacatcg gtgtccccca tggggggttc cccgaaccct
     4261 ttcgctctaa ggtactgaag gacctgccaa gggtggaggg gcggcctgga gcctccctcc
     4321 ctcccctgga tctgcaggca ctggagaagg agctggtaga ccggcatggg gaggaggtga
     4381 cgccggaaga tgtgctctca gcagctatgt accccgatgt gtttgcccac ttcaaggact
     4441 tcactgccac ctttggcccc ctggatagcc tgaatactcg cctcttcctg cagggaccca
     4501 agatcgcaga ggagtttgag gtggagctgg agcggggcaa gacgctgcac atcaaagccc
     4561 tggccgtgag cgacctgaac cgggccggcc agaggcaggt cttctttgag ctcaatgggc
     4621 agctgcggtc catcttggtc aaggacaccc aggccatgaa ggagatgcac ttccacccca
     4681 aggccctaaa ggacgtgaag ggccagatcg gggcgcccat gcctgggaag gtgatagaca
     4741 tcaaagtggt ggcaggggcc aaggtggcca agggccagcc cctgtgtgtg ctcagtgcca
     4801 tgaagatgga gactgtggtg acctcaccca tggagggtac tgtccgcaag gttcatgtga
     4861 ccaaggacat gacactggaa ggtgacgacc tcatcctgga gatcgaggaa caaaaactca
     4921 tctcagaaga ggatctgtga gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt
     4981 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5041 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5101 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5221 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5281 tttggaggcc taggcttttg caaaaagctt cgcatgattg aacaagatgg attgcacgca
     5341 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5401 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5461 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5521 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5581 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5641 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5701 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5761 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5821 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5881 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5941 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6001 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6061 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat cgataaaata
     6121 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     6181 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     6241 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6301 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     6361 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     6421 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     6481 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     6541 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     6601 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     6661 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     6721 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt
     6781 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
     6841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7741 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7801 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7861 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7921 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7981 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8041 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8101 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8161 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8221 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8281 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8341 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8401 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8461 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8521 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8581 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8641 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8701 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8761 acatttcccc gaaaagtgcc ac
//
