LOCUS       sequence-388332-        7341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting PSD-95 MAGUK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7341)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PSD-95/GW1
FEATURES             Location/Qualifiers
     source          1..7341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        552..931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        932..1135
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1086..1106
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1132..1156
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1271..2097
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     polyA_signal    4480..4614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4517..4536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4571..4590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4637..4653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4637..4653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4650..4672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4661..4677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4685..4691,4692..4709,4710..4715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4867..4884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5038..5626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5118..5137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5797..6588,6589..6657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6420..6439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     6867..6885
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6923..6945)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7045..7064
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7296..7318
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7310..7327
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7311..7327
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cggtgactgc agtgaataat aaaatgtgtg tttgtccgaa atacgcgttt tgagatttct
       61 gtcgccgact aaattcatgt cgcgcgatag tggtgtttat cgccgataga gatggcgata
      121 ttggaaaaat cgatatttga aaatatggca tattgaaaat gtcgccgatg tgagtttctg
      181 tgtaactgat atcgccattt ttccaaaagt gatttttggg catacgcgat atctggcgat
      241 agcgcttata tcgtttacgg gggatggcga tagacgactt tggtgacttg ggcgattctg
      301 tgtgtcgcaa atatcgcagt ttcgatatag gtgacagacg atatgaggct atatcgccga
      361 tagaggcgac atcaagctgg cacatggcca atgcatatcg atctatacat tgaatcaata
      421 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      481 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      541 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      601 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      661 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      721 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      781 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      841 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      901 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      961 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1021 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1081 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     1141 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     1201 ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc
     1261 caagagtgac gtaagtaccg cctatagagt ctataggccc acccccttgg cttcttatgc
     1321 atgctatact gtttttggct tggggtctat acacccccgc ttcctcatgt tataggtgat
     1381 ggtatagctt agcctatagg tgtgggttat tgaccattat tgaccactcc cctattggtg
     1441 acgatacttt ccattactaa tccataacat ggctctttgc cacaactctc tttattggct
     1501 atatgccaat acactgtcct tcagagactg acacggactc tgtattttta caggatgggg
     1561 tctcatttat tatttacaaa ttcacatata caacaccacc gtccccagtg cccgcagttt
     1621 ttattaaaca taacgtggga tctccacgcg aatctcgggt acgtgttccg gacatgggct
     1681 cttctccggt agcggcggag cttctacatc cgagccctgc tcccatgcct ccagcgactc
     1741 atggtcgctc ggcagctcct tgctcctaac agtggaggcc agacttaggc acagcacgat
     1801 gcccaccacc accagtgtgc cgcacaaggc cgtggcggta gggtatgtgt ctgaaaatga
     1861 gctcggggag cgggcttgca ccgctgacgc atttggaaga cttaaggcag cggcagaaga
     1921 agatgcaggc agctgagttg ttgtgttctg ataagagtca gaggtaactc ccgttgcggt
     1981 gctgttaacg gtggagggca gtgtagtctg agcagtactc gttgctgccg cgcgcgccac
     2041 cagacataat agctgacaga ctaacagact gttcctttcc atgggtcttt tctgcagtca
     2101 ccgtccttga cacgaagctt gatgcaaaac tccaatgaag tcagagcccc ctactcgccg
     2161 ccgcggccag gccccccaac atggactgtc tctgtatagt gacaaccaag aaataccgct
     2221 accaagatga agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca
     2281 attctccccc tgtgattgtc aacacggaca ccctagaagc cccaggatat gagttgcagg
     2341 tgaatggaac agagggggag atggagtatg aggagatcac attggaaagg ggtaactcag
     2401 gtctgggctt cagcatcgca ggtggcactg acaacccgca catcggtgac gacccgtcca
     2461 tttttatcac caagatcatt cctggtgggg ctgcagccca ggatggccgc ctcagggtca
     2521 atgacagcat cctgtttgta aatgaagtgg atgttcggga ggtgacccat tcagctgcgg
     2581 tggaggccct caaagaggca ggttccatcg ttcgcctcta tgtcatgcgc cggaaacccc
     2641 cagccgaaaa ggtcatggag atcaaactca tcaaagggcc taaaggactt ggcttcagca
     2701 ttgcgggggg cgttgggaac cagcacatcc ctggagataa cagcatctat gtaacgaaga
     2761 tcatcgaagg aggtgctgcc cacaaggatg gcaggttgca gattggagac aagatcctgg
     2821 cggtcaacag tgtggggctg gaggacgtca tgcacgagga tgccgtggca gccctgaaga
     2881 acacatatga cgttgtgtac ctaaaggtgg ccaagcccag caatgcctac ctgagtgaca
     2941 gctatgctcc cccagacatc acaacctcgt attctcagca cctggacaat gagatcagtc
     3001 atagcagcta cttgggcact gactacccca cagccatgac ccccacttcc cctcggcgct
     3061 actcccctgt ggccaaggac ctgctggggg aggaagacat tccccgggaa ccaaggcgga
     3121 tcgtgatcca tcggggctcc accggcctgg gcttcaacat cgtgggcggc gaggatggtg
     3181 aaggcatctt catctccttc atccttgctg ggggtccagc cgacctcagt ggggagctac
     3241 ggaaggggga ccagatcctg tcggtcaatg gtgttgacct ccgcaatgcc agtcacgaac
     3301 aggctgccat tgccctgaag aatgcgggtc agacggtcac gatcatcgct cagtataaac
     3361 cagaagagta tagtcgattc gaggccaaga tccatgatct tcgggaacag ctcatgaata
     3421 gtagcctagg ctcagggact gcatccttgc gaagcaaccc caagaggggc ttctacatta
     3481 gggccctgtt tgattacgac aagaccaagg actgcggttt cttgagccag gccctgagct
     3541 tccgcttcgg ggatgtgctt catgtcattg acgctggtga cgaagagtgg tggcaagcac
     3601 ggcgggtcca ctccgacagt gagaccgacg acattggctt cattcccagc aaacggcggg
     3661 tcgagcgacg agagtggtca aggttaaagg ccaaggactg gggctccagc tctggatcac
     3721 agggtcgaga agactcggtt ctgagctatg agacggtgac ccagatggaa gtgcactatg
     3781 ctcgtcccat catcatcctt ggacccacca aagaccgtgc caacgatgat cttctctccg
     3841 agttccccga caagtttgga tcctgtgtcc ctcatacgac acgtcctaag cgggaatatg
     3901 agatagacgg ccgggattac cactttgtct cctcccggga gaaaatggag aaggacatcc
     3961 aggcacacaa gttcattgag gctggccagt acaacagcca cctctatggg accagcgtcc
     4021 agtctgtgcg agaggtagca gagcagggga agcactgcat cctcgatgtc tcggccaatg
     4081 ccgtgcggcg gctgcaggcg gcccacctgc accccatcgc catcttcatc cgtccccgct
     4141 ccctggagaa tgtgctagag atcaataagc ggatcacaga ggagcaagcc cggaaagcct
     4201 tcgacagagc cacgaagctg gagcaggagt tcacagagtg cttctcagcc atcgtagagg
     4261 gcgacagctt tgaagagatc tatcacaaag tgaaacgtgt cattgaagac ctctcaggcc
     4321 cctacatctg ggtcccagcc cgagagagac tctgattcct gatcgaattc attgatcata
     4381 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4441 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4561 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgtacc
     4621 gagctcgcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4681 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4741 aactacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5581 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5641 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5701 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6661 cttccttttt caataagcgg ccgcggccat gccggccact agtctcgagt tattattgaa
     6721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6841 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     6901 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6961 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7021 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7081 tgaagacgtc gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     7141 ctgcataaat aaaaaaaatt agtcagggcg ccattcgcca ttcaggctgc gcaactgttg
     7201 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7261 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7321 ggccagtgcc cgtgtcaagg a
//
