LOCUS       sequence-388335-       15078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wild-type Homo sapiens ATP13A3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Houdou M, Jacobs N, Coene J, Azfar M, Vanhoutte R, Van den Haute C, 
            Eggermont J, Daniels V, Verhelst SHL, Vangheluwe P
  TITLE     Novel Green Fluorescent Polyamines to Analyze ATP13A2 and ATP13A3 
            Activity in the Mammalian Polyamine Transport System.
  JOURNAL   Biomolecules. 2023 Feb 9;13(2):337. doi: 10.3390/biom13020337.
  PUBMED    36830711
REFERENCE   2  (bases 1 to 15078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2023-02-9"; volume: "13(2):337. doi"; pages:
            " 10.3390/biom13020337"
COMMENT     Sequence Label: pLenti HsATP13A3 WT
FEATURES             Location/Qualifiers
     source          1..15078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             92..725
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    772..897
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1390..1623
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1808..1852
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2145..2262
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2321..2624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2625..2828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2779..2799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2825..2849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2917..2926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6657..7209
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6801..6818)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7028..7047
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7209..7811
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7708..7728
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7831..8419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7884..7904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8302..8313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8589..8769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10297..10316)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10530..10549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10649..10671
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10709..10727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10795..10899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10900..10968,10969..11760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11118..11137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11931..12519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12420..12439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12673..12690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12760..12780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12765..13094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12945..13080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          14228..14293
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14423..14443
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14868..15002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14905..14924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14959..14978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
       61 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
      121 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
      181 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
      241 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
      301 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
      361 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
      421 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
      481 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      541 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      601 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      661 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      721 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      781 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      841 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      901 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      961 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1021 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1081 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1141 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1201 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1261 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1321 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1381 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1441 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1501 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1561 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1621 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1681 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1741 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1801 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1861 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1921 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1981 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2041 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2101 tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2161 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2221 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2281 agcagagatc cactttgggg cgataagctt gggagttccg cgttacataa cttacggtaa
     2341 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2401 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2461 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2521 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2581 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2641 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2701 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2761 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2821 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2881 tccatagaag acaccgactc tagaggatcc accggtgcca ccatggacag ggaagaaagg
     2941 aagaccatca atcagggtca agaagatgaa atggagattt atggttacaa tttgagtcgc
     3001 tggaagcttg ccatagtttc tttaggagtg atttgctctg gtgggtttct cctcctcctc
     3061 ctctattgga tgcctgagtg gcgggtgaaa gcgacctgtg tcagagctgc aattaaagac
     3121 tgtgaagtag tgctgctgag gactactgat gaattcaaaa tgtggttttg tgcaaaaatt
     3181 cgcgttcttt ctttggaaac ttacccagtt tcaagtccaa aatctatgtc taataagctt
     3241 tcaaatggcc atgcagtttg tttaattgag aatcccactg aagaaaatag gcacaggatc
     3301 agtaaatatt cacagactga atcacaacag attcgttatt tcacccacca tagtgtaaaa
     3361 tatttctgga atgataccat tcacaatttt gatttcttaa agggactgga tgaaggtgtt
     3421 tcttgtacgt caatttatga aaagcatagt gcaggactga caaaggggat gcatgcctac
     3481 agaaaactgc tttatggagt aaatgaaatt gctgtaaaag tgccttctgt ttttaagctt
     3541 ctaattaaag aggttctcaa cccattttac attttccagc tgttcagtgt tatactgtgg
     3601 agcactgatg aatactatta ctatgctcta gctattgtgg ttatgtccat agtatcaatc
     3661 gtaagctcac tatattccat tagaaagcaa tatgttatgt tgcatgacat ggtggcaact
     3721 catagtaccg taagagtttc agtttgtaga gtaaatgaag aaatagaaga aatcttttct
     3781 accgaccttg tgccaggaga tgtcatggtc attccattaa atgggacaat aatgccttgt
     3841 gatgctgtgc ttattaatgg tacctgcatt gtaaacgaaa gcatgttaac aggagaaagt
     3901 gttccagtga caaagactaa tttgccaaat ccttcagtgg atgtgaaagg aataggagat
     3961 gaattatata atccagaaac acataaacga catactttgt tttgtgggac aactgttatt
     4021 cagactcgtt tctacactgg agaactcgtc aaagccatag ttgttagaac aggatttagt
     4081 acttccaaag gacagcttgt tcgttccata ttgtatccca aaccaactga ttttaaactc
     4141 tacagagatg cctacttgtt tctactatgt cttgtggcag ttgctggcat tgggtttatc
     4201 tacactatta ttaatagcat tttaaatgag gtacaagttg gggtcataat tatcgagtct
     4261 cttgatatta tcacaattac tgtgccccct gcacttcctg ctgcaatgac tgctggtatt
     4321 gtgtatgctc agagaagact gaaaaaaatc ggtattttct gtatcagtcc tcaaagaata
     4381 aatatttgtg gacagctcaa tcttgtttgc tttgacaaga ctggaactct aactgaagat
     4441 ggtttagatc tttgggggat tcaacgagtg gaaaatgcac gatttctttc accagaagaa
     4501 aatgtgtgca atgagatgtt ggtaaaatcc cagtttgttg cttgtatggc tacttgtcat
     4561 tcacttacaa aaattgaagg agtgctctct ggtgatccac ttgatctgaa aatgtttgag
     4621 gctattggat ggattctgga agaagcaact gaagaagaaa cagcacttca taatcgaatt
     4681 atgcccacag tggttcgtcc tcccaaacaa ctgcttcctg aatctacccc tgcaggaaac
     4741 caagaaatgg agctgtttga acttccagct acttatgaga taggaattgt tcgccagttc
     4801 ccattttctt ctgctttgca acgtatgagt gtggttgcca gggtgctggg ggataggaaa
     4861 atggacgcct acatgaaagg agcgcccgag gccattgccg gtctctgtaa acctgaaaca
     4921 gttcctgtcg attttcaaaa cgttttggaa gacttcacta aacagggctt ccgtgtgatt
     4981 gctcttgcac acagaaaatt ggagtcaaaa ctgacatggc ataaagtaca gaatattagc
     5041 agagatgcaa ttgagaacaa catggatttt atgggattaa ttataatgca gaacaaatta
     5101 aagcaagaaa cccctgcagt acttgaagat ttgcataaag ccaacattcg caccgtcatg
     5161 gtcacaggtg acagtatgtt gactgctgtc tctgtggcca gagattgtgg aatgattcta
     5221 cctcaggata aagtgattat tgctgaagca ttacctccaa aggatgggaa agttgccaaa
     5281 ataaattggc attatgcaga ctccctcacg cagtgcagtc atccatcagc aattgaccca
     5341 gaggctattc cggttaaatt ggtccatgat agcttagagg atcttcaaat gactcgttat
     5401 cattttgcaa tgaatggaaa atcattctca gtgatactgg agcattttca agaccttgtt
     5461 cctaagttga tgttgcatgg caccgtgttt gcccgtatgg cacctgatca gaagacacag
     5521 ttgatagaag cattgcaaaa tgttgattat tttgttggga tgtgtggtga tggcgcaaat
     5581 gattgtggtg ctttgaagag ggcacacgga ggcatttcct tatcggagct cgaagcttca
     5641 gtggcatctc cctttacctc taagactcct agtatttcct gtgtgccaaa ccttatcagg
     5701 gaaggccgtg ctgctttaat aacttccttc tgtgtgttta aattcatggc attgtacagc
     5761 attatccagt acttcagtgt tactctgctg tattctatct taagtaacct aggagacttc
     5821 cagtttctct tcattgatct ggcaatcatt ttggtagtgg tatttacaat gagtttaaat
     5881 cctgcctgga aagaacttgt ggcacaaaga ccaccttcgg gtcttatatc tggggccctt
     5941 ctcttctccg ttttgtctca gattatcatc tgcattggat ttcaatcttt gggttttttt
     6001 tgggtcaaac agcaaccttg gtatgaagtg tggcatccaa aatcagatgc ttgtaataca
     6061 acaggaagcg ggttttggaa ttcttcacac gtagacaatg aaaccgaact tgatgaacat
     6121 aatatacaaa attatgaaaa taccacagtg ttttttattt ccagttttca gtacctcata
     6181 gtggcaattg ccttttcaaa aggaaaaccc ttcaggcaac cttgctacaa aaattatttt
     6241 tttgtttttt ctgtgatttt tttatatatt tttatattat tcatcatgtt gtatccagtt
     6301 gcctctgttg accaggttct tcagatagtg tgtgtaccat atcagtggcg tgtaactatg
     6361 ctcatcattg ttcttgtcaa tgcctttgtg tctatcacag tggaggagtc agtggatcgg
     6421 tggggaaaat gctgcttacc ctgggccctg ggctgtagaa agaagacacc aaaggcaaag
     6481 tacatgtatc tggcgcagga gctcttggtt gatccagaat ggccaccaaa acctcagaca
     6541 accacagaag ctaaagcttt agttaaggag aatggatcat gtcaaatcat caccataaca
     6601 tagctcgaga ctagtacgcg tgctctagcc caattccgcc ccctcccccc cccctaacgt
     6661 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6721 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     6781 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     6841 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     6901 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     6961 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     7021 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7081 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7141 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7201 atgataatat gggcaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     7261 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     7321 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     7381 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7441 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7501 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7561 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7621 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7681 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7741 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7801 ccggtgcctg aggcgcgttg aattcccgat aatcaacctc tggattacaa aatttgtgaa
     7861 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7921 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7981 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8041 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8101 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8161 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8221 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8281 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8341 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8401 ctttgggccg cctccccgca tcgggaattc tgtcgactgg tacctttaag accaatgact
     8461 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8521 attcactccc aacgaagaca aaatcgtcga gagatgctgc atataagcag ctgctttttg
     8581 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8641 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8701 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8761 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8821 gaatatcaga gagtgagagg ccttgacatt ataatagatt tagcaggaat tgaactagga
     8881 gtggagcaca caggcaaagc tgcagaagta cttggaagaa gccaccagag atactcacga
     8941 ttctgcacat acctggctaa tcccagatcc taaggattac attaagttta ctaacattta
     9001 tataatgatt tatagtttaa agtataaact tatctaattt actattctga cagatattaa
     9061 ttaatcctca aatatcataa gagatgatta ctattatccc catttaacac aagaggaaac
     9121 tgagagggaa agatgttgaa gtaattttcc cacaattaca gcatccgtta gttacgactc
     9181 tatgatcttc tgacacaaat tccatttact cctcacccta tgactcagtc gaatatatca
     9241 aagttatgga cattatgcta agtaacaaat taccctttta tatagtaaat actgagtaga
     9301 ttgagagaag aaattgtttg caaacctgaa tagcttcaag aagaagagaa gtgaggataa
     9361 gaataacagt tgtcatttaa caagttttaa caagtaactt ggttagaaag ggattcaaat
     9421 gcataaagca agggataaat ttttctggca acaagactat acaatataac cttaaatatg
     9481 acttcaaata attgttggaa cttgataaaa ctaattaaat attattgaag attatcaata
     9541 ttataaatgt aatttacttt taaaaaggga acatagaaat gtgtatcatt agagtagaaa
     9601 acaatcctta ttatcacaat ttgtcaaaac aagtttgtta ttaacacaag tagaatactg
     9661 cattcaatta agttgactgc agattttgtg ttttgttaaa attagaaaga gataacaaca
     9721 atttgaatta ttgaaagtaa catgtaaata gttctacata cgttcttttg acatcttgtt
     9781 caatcattga tcgaagttct ttatcttgga agaatttgtt ccaaagactc tgaaataagg
     9841 aaaacaatct attatatagt ctcacacctt tgttttactt ttagtgattt caatttaata
     9901 atgtaaatgg ttaaaattta ttcttctctg agatcatttc acattgcaga tagaaaacct
     9961 gagactgggg taatttttat taaaatctaa tttaatctca gaaacacatc tttattctaa
    10021 catcaatttt tccagtttga tattatcata taaagtcagc cttcctcatc tgcaggttcc
    10081 acaacaaaaa tccaaccaac tgtggatcaa aaatattggg aaaaaattaa aaatagcaat
    10141 acaacaataa aaaaatacaa atcagaaaaa cagcacagta taacaacttt atttagcatt
    10201 tacaatctat taggtattat aagtaatcta gccagatcct ctacgccgga cgcatcgtgg
    10261 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
    10321 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
    10381 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
    10441 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
    10501 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    10561 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
    10621 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
    10681 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    10741 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    10801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    11041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    11101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    11161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    11461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    11521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    11581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    11641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    11761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    11821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    12001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    12121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    12721 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
    12781 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    12841 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    12901 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    12961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    13021 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    13081 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
    13141 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
    13201 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
    13261 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
    13321 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
    13381 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
    13441 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
    13501 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
    13561 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
    13621 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
    13681 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
    13741 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
    13801 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
    13861 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
    13921 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
    13981 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
    14041 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
    14101 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
    14161 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
    14221 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
    14281 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
    14341 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
    14401 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
    14461 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
    14521 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
    14581 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
    14641 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
    14701 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
    14761 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
    14821 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    14881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    14941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    15001 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    15061 ccactgccaa ttacctgt
//
