LOCUS       sequence-388345-        1515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human superstable p53 as His6GB1tev fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     Mapping Interactions of the Intrinsically Disordered C-Terminal 
            Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.
  JOURNAL   Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 
            10.1021/acs.biochem.2c00528. Epub 2022 Nov 15.
  PUBMED    36380579
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.2c00528"; journalName: "Biochemistry"; date: 
            "2022-12-6- 6"; volume: "61"; issue: "23"; pages: "2709-2719"
COMMENT     Sequence Label: H6GB1tevP53SS(1-393)
FEATURES             Location/Qualifiers
     source          1..1515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..239
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             264..284
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             291..1472
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKLFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCYSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
ORIGIN
        1 ctagaataat tttgtttact ttaagaagga gatataccat gggccatcat catcatcatc
       61 acagcagcgg catgacttac aaactgatcc ttaacggtaa aactttgaaa ggcgaaacca
      121 ctaccgaagc tgttgatgct gcgactgcag aaaaagtttt caaacagtac gctaacgaca
      181 acggtgttga cggtgaatgg acttacgacg atgcgactaa gaccttcacc gttactgaag
      241 aattcagcag cggcagcagc ggcgaaaacc tgtattttca gggctcccat atggaggagc
      301 cgcagtcaga tcctagcgtc gagccccctc tgagtcagga aacattttca gacctatgga
      361 aactacttcc tgaaaacaac gttctgtccc ccttgccgtc ccaagcaatg gatgatttga
      421 tgctgtcccc ggacgatatt gaacaatggt tcaccgaaga cccaggtcca gatgaagctc
      481 ccagaatgcc agaggctgct ccccccgtgg cccctgcacc agcagctcct acaccggcgg
      541 cccctgcacc agccccctcc tggcccctgt catcttctgt cccttcccag aaaacctacc
      601 agggcagcta cggtttccgt ctgggcttct tgcattctgg gacagccaag tctgtgactt
      661 gcacgtactc ccctgccctc aacaagctgt tttgccaact ggccaagacc tgccctgtgc
      721 agctgtgggt tgattccaca cccccgcccg gcacccgcgt ccgcgccatg gccatctaca
      781 agcagtcaca gcacatgacg gaggttgtga ggcgctgccc ccaccatgag cgctgctcag
      841 atagcgatgg tctggcccct cctcagcatc ttatccgagt ggaaggaaat ttgcgtgcag
      901 agtatttgga tgacagaaac acttttcgac atagtgtggt ggtgccctat gagccgcctg
      961 aggttggctc tgactgtacc accatccact acaactacat gtgttatagt tcctgcatgg
     1021 gcggcatgaa ccggaggccc atcctcacca tcatcacact ggaagactcc agtggtaatc
     1081 tactgggacg ggatagcttt gaggtgcgtg tttgtgcctg tcctgggaga gaccggcgca
     1141 cagaggaaga gaatctccgc aagaaagggg agcctcacca cgagctgccc ccagggagca
     1201 ctaagcgagc actgcccaac aacaccagct cctctcccca gccaaagaag aaaccactgg
     1261 atggagaata tttcaccctt cagatccgtg ggcgtgagcg cttcgagatg ttccgagagc
     1321 tgaatgaggc cttggaactc aaggatgccc aggctgggaa ggagccaggg gggagcaggg
     1381 ctcactccag ccacctgaag tccaaaaagg gtcagtctac ctcccgccat aaaaaactca
     1441 tgttcaagac agaagggcct gactcagact aataggatcc gaattcgagc tccgtcgaca
     1501 agcttgcggc cgcac
//
