LOCUS       sequence-388346-       11415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLX304 backbone with an IRES-Tdtomato cassette subcloned into the 
            NheI-AgeI sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, 
            Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, 
            Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi 
            SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
  TITLE     Targeting oncoproteins with a positive selection assay for protein 
            degraders.
  JOURNAL   Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 
            10.1126/sciadv.abd6263. Print 2021 Feb.
  PUBMED    33547076
REFERENCE   2  (bases 1 to 11415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-5"; volume: "7(6). pii: 7/6/eabd6263. doi"; pages: " 
            10.1126/sciadv.abd6263. Print 2021 Feb"
COMMENT     Sequence Label: pLX304-IRES-Td
FEATURES             Location/Qualifiers
     source          1..11415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..719
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(744..749,750..767,768..774)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             828..1487
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(894..913)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1829..2134
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2013..2032
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2175..2299)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2301..2342
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(2382..2956,2957..2959,2960..2968)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2549..2566)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2776..2795
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2969..4399
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    4422..5010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4475..4495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4893..4904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5082..5315
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5393..5514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5430..5449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5484..5503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5554..5689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5709..5728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5710..5728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5738..5755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5738..5754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5747..5769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5896..6351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5983..6002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6193..6214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6377..6481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6482..6550,6551..7342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6700..6719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7513..8101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8002..8021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8255..8272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8389..8410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8425..8430,8431..8448,8449..8455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8463..8479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8468..8490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8487..8503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8487..8503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8522..8542
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8524..8542
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8570..8796
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8797..8977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9024..9149
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9642..9875
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10060..10104
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10279..10779
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10698..10716
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10791..11186
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11246..11363
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      661 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
      721 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
      781 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      841 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      901 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      961 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1021 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     1081 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1141 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1201 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1261 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1321 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1381 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1441 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg
     1501 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     1561 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     1621 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     1681 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     1741 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     1801 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     1861 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     1921 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     1981 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     2041 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     2101 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     2161 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     2221 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     2281 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     2341 cgtagtaatg agctagccgc tacgtaaatt ccgccccccc ccccctctcc ctcccccccc
     2401 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     2461 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     2521 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     2581 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     2641 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     2701 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     2761 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     2821 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     2881 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     2941 aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagg tcatcaaaga
     3001 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     3061 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3121 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     3181 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     3241 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     3301 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     3361 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     3421 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     3481 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     3541 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     3601 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     3661 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     3721 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     3781 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3841 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     3901 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     3961 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     4021 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     4081 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     4141 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     4201 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     4261 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     4321 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     4381 ggacgagctg tacaagtaaa ccggtggcgc gttaagtcga caatcaacct ctggattaca
     4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4801 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4981 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     5041 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5101 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5161 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5221 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5281 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     5341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5521 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5581 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5641 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     5701 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     5761 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5821 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5881 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5941 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6001 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6061 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6121 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6181 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6241 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6301 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8161 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8221 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8281 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8341 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8401 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8461 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8521 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     8581 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     8641 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     8701 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     8761 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     8821 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8881 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8941 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9001 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     9061 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     9121 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     9181 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     9241 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     9301 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9361 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     9421 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     9481 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9541 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9601 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9661 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9721 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9781 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9841 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9901 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9961 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    10021 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    10081 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    10141 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    10201 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    10261 ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
    10321 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
    10381 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
    10441 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
    10501 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
    10561 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
    10621 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
    10681 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
    10741 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc
    10801 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
    10861 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
    10921 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
    10981 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
    11041 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
    11101 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
    11161 tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaagaattc tagatcttga
    11221 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    11281 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    11341 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    11401 gccggctcga ggggg
//
