LOCUS       sequence-388349-       14857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HuC driven expression of the inactive calcium recorder negative 
            control Caprola_off fused to mEGFP for neuronal expression in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N,
            Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, 
            Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
  TITLE     Recording physiological history of cells with chemical labeling.
  JOURNAL   Science. 2024 Feb 23;383(6685):890-897. doi: 
            10.1126/science.adg0812. Epub 2024 Feb 22.
  PUBMED    38386755
REFERENCE   2  (bases 1 to 14857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg0812"; journalName: "Science"; date: 
            "2024-02-23- 23"; volume: "383"; issue: "6685"; pages: "890-897"
COMMENT     Sequence Label: pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP
FEATURES             Location/Qualifiers
     source          1..14857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2610..3050
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             3171..3227
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHALRAIGRLSS"
     CDS             join(4131..4133,4134..4136,4137..4850)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4176..4197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4437..4456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4784..4805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4892..5026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4916..4935)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4970..4989
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5465..5652,5653..5664)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5698..5714)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5698..5714)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5751..5771)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5751..5769)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5790..5806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5790..5806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5803..5825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5814..5830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5838..5844,5845..5862,5863..5868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5883..5904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6021..6038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6192..6780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6272..6291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6951..7742,7743..7811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7574..7593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7812..7916)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8079..8100)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8449..8460,8461..8623)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 aattacttta tctcatgctg ctttcaggcc tctgcttatt ttattattat tattttaaat
       61 tagggggaat cacggtggcg ttgtgggtag ggtagcgcga tcaccttaca gcaagaaggt
      121 cgctggttcg agctctggct gggtcagttg gcatttctgt gtggagttta aattgaattg
      181 aaattgaata aactaaattg gccctagtgt atgtgtgtga atgtaagtgt gcatgggtgt
      241 ttcccagtgt tgggtttgac tggaagagca tccgcttcgt aaaacatatg ctggataagt
      301 tggcggttca tttcactgtg gtgaccgctg attaataaag ggactaagct gaaaatgaaa
      361 tgaattaata cattttaaat gagcttttgc tgacatgttt atgattacaa atagtgaaat
      421 tgtatgttaa tttacgataa gtgcattgtt ctaaattcat ttttatgtag cagctggttt
      481 ttcttccatt tgtgtcattc aacagaacat ttttctaaaa aagtgaactt aaattttctc
      541 ctccctctga caaggcttga tggtaatttc agccgactct aattacagac tcactaaaag
      601 gaatcggtgc agtcatttat ctttatcagc gcgctggtgg gaaggtaata ggttttgctt
      661 atgagttgtt tgcgtgcgag tctgggtcct tgtgtaggcg atgctgatgc actgcattaa
      721 atattgaggg agaagtcgct caaaaatagc aaagtacagg ctggggcagg aacgtcaaag
      781 cccgctcagt ggccacgttt cttgtttcgt aagtcctatt tttaatagcg ataattcagg
      841 ctcagtagaa ggaactccag cactataagc agtctctcgc ctgtctcggc gagttatgta
      901 ttttgggatg aagtgctctt ccacctctac ctctgctgtg ctggcgccag tatctgggct
      961 gtaagatagt gtacgtgtca gtctgtgtgg ccgaagagcc tgtgtgttgt gtaactcact
     1021 acattcgtcg ctgtggatgg ctgacttgcc atggggaggt ttacttcagt tcacgaatca
     1081 cattcgccac gatcttgtca ctttgtgtca agactcgctc tctgtgtgtc aacagctctg
     1141 ctgtttgagg ttgaaattct gaacttgaga tatatcccag gatatattag ggctactgcc
     1201 aacggaaaaa gcaaatggtg ccatggccat ttgattgatc aaacaaacac ccttttgctt
     1261 gggtgacagt gaaatgtcag atccatgttt gtactgcttt aataacttgc ctctctgagc
     1321 tttctccttg tcagtgtcaa tttgcctgag atataaattc cggttgcccc gaaagcttat
     1381 tttattaaaa aaaagctaac atgcactcat acaacgtgcg cacatacatg tgaggcttgc
     1441 taaaagtcaa gaagcaagcc aatagcaaga gtcgtcagac tgctgttacc tgggcaacct
     1501 ggttagaggt aaggctcaag gcaagaataa atcattccac tgaagagaca cagcgttaca
     1561 gcgagatgag agcaagaggc gatagtgaag ggagaacaaa agaacagcct acccacacta
     1621 gtgcagatct acctgtttga gctactgatt gttcagtttg attagatatc agacaattct
     1681 gctatctcca attgcattgc tgcatgaatt cctagaaatc attttaatca ccaaaaacta
     1741 gtagggacac ttgtggcgta tatgattctg cctgttgatt gtggggatcc aacagaaaga
     1801 tcatgaatgc attgttaatt aagtcggtga gacaccctgt tccaccctag gggccgttct
     1861 ggggaaagag tgctttcagt cagctaaaga tgactaatat gtgaacatat attactacac
     1921 atgccaattt gtacattctg gatagttacc agccctggga aaacatcgaa aaaaataaat
     1981 caacatagat aaaattggca aatcccttct ggtacgatgc agagtgcttg ctctgagaac
     2041 ttgtgcattg tgacagaaag cgaccgttaa aaactgaaag tagcatctac aacctgcata
     2101 catctgttct ttgaaaatcc cctgtaggcc aaattaagca tcacgctgtg ctccacagca
     2161 acacggatga accgaaaacc cagacacaaa cgcacacact tttcacataa acaaggttta
     2221 gttatgctag ctatgctttt tcgtcctccc tttgtcatca gcggcgagtg acgtaacaca
     2281 gtttgaccct ggacagcgta aagggggggt tgggaatgag tgaaaggact gtgacatctg
     2341 cagggccagg ggctgatgga gagagctgaa gtgagggagg tcagaggagg aggaagggtg
     2401 ggatattgtc ctcctcaggc cctatccctc tcagacgcat tgatttttaa cctctcagcg
     2461 agaatgccaa gcattacacc cctcaaatta tggttcctca cccaacctgt tatattttcc
     2521 acctgcagat aattaccggt atgctgcaga acgagcttgc tcttaagttg gctggacttg
     2581 atattaacaa gactggaggt tctctgccgg accaactgac tgaagagcag atcgcagaat
     2641 ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca accaaggagc
     2701 tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg caggacatga
     2761 tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc ctgacaatga
     2821 tggcaagaaa aatgaaagac acagacagtg aagaagaaat tagagaagcg ttccgtgtgt
     2881 ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg atgacaaacc
     2941 ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca gacatcgatg
     3001 gggatggtca ggtaaactac gaagagtttg tagtgatgat gacagcgaag gaattccctc
     3061 ctcctccgcc accgccacca ccgccgccgc cgccaccacc acctcctccg cctcctccac
     3121 caccaccgcc accgcctcca ccacctggcg gcagcagggt cgactcatca cgtcgtaagt
     3181 ggaataagac aggtcacgca ctgagagcta taggtcggct gagctcactc gagggcggtt
     3241 ctgacgtcgg ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga
     3301 tgggtgtcgt ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga
     3361 atcctgttga ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc
     3421 cagcgaacat cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc
     3481 cgaagctgct gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc
     3541 tggccaaaag cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc
     3601 aagaagacaa cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga
     3661 ttggtggcac cggtggctct ggcggtaccg gtggctctgg cggttctatc ggtactggct
     3721 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     3781 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     3841 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
     3901 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     3961 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     4021 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     4081 ttatggagtt catccgccct atcccgacct gggacgaatg gggaggttct atggtgagca
     4141 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4201 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4261 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4321 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4381 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4441 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4501 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4561 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4621 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4681 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4741 cccagtccaa actgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4801 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ccggtggatc
     4861 ctggccgtcg ttttactgta ccatcgatga tgatccagac atgataagat acattgatga
     4921 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4981 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5041 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5101 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctgcg aagatacggc
     5161 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
     5221 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
     5281 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
     5341 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
     5401 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
     5461 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
     5521 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
     5581 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
     5641 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
     5701 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     5761 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5821 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5881 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5941 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6001 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6061 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6841 ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7921 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7981 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     8041 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     8101 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     8161 cacgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa
     8221 ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact
     8281 acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt
     8341 gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca
     8401 tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa
     8461 aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat
     8521 taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta
     8581 aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct
     8641 tgtttaagct tcccgggtgt cgacgaattc tcgagctcta ttcctaaaga ccttgggtga
     8701 ccaaaatctt attttaataa ataaaactgt ttattaaaac ttttttgttt caaagaacca
     8761 tatgtatagt gaaatttata aaaatatcaa tttttaaaaa gctggtgtac tcatttatgt
     8821 tatgaactct aaaaccatat actgactgca agtgatgatg tatagagtga tgtttacgag
     8881 taaacatatt tagttgtata catcctactg agcacatttt gatgtatgaa ataacattac
     8941 aagctttatc caaattaagc cattttaaaa cactgccaat tgaaaataca aatcctggaa
     9001 aaaatcgtct ttagcgcagt catttgagcc atcctaatcc gttacctcag accataataa
     9061 gaagggataa cactagctgt agcaatggaa cacatctgtt tcacacaatc atatctcctg
     9121 cgccggtgct aagcagattc agcgtgatca taacatgctt tccactcata aatgtaaatt
     9181 tacaatttgc acatgtaaaa cagacacttt tgagatattg gataaaaaaa caagagtata
     9241 ttgcttagtt tcatccacca gtcatcccca cagcgtttgg aaggccataa aaagtgtcta
     9301 aaatcaatga tcattgaaag agcacaagag agactcttac gctgtaatgc cactggggac
     9361 aaaagtgaca gtctcttaat gggctcttct ggaggggctc ctgaacatta aaaattatca
     9421 gcgaaattac cgaaagagct tcaagcaact ggcatgcttg atcctctgcg tcggggcggt
     9481 gaataggtgc ttcagatgcc ctcttaccca cgggctggat tcagctgccc cgctaccagc
     9541 ggagaccccc taatgagcct ctgcaattaa gtttattcat gttaagtgtg aacggggtgc
     9601 gtgcggaact gtgggcagct aacagacctg ggttctttgt gccacaagtg ctgcctttat
     9661 tcggctcaca aagcagaaaa caacacccgc acctataatg gcgccctcgg ctgggtctaa
     9721 gaaacgtggc gagttgacag agcagagtgg gcggggttaa gacagactga cagcgggacc
     9781 catctccatc ctcttattaa cgcttaacga gtgccttcct catgcaatat tcatcgccac
     9841 taatatcatc caagctctga gctgagctgg ccacttatgt aaggcaatta tgtaaaatat
     9901 cagacagggc ccacactcag aatctgactg gggtagagac gcgggacgag aaccgagagc
     9961 aagaactgaa agtgaaagtg accactaaag ggaggagagg acagaggggc aggatgtgtc
    10021 aagattacca gagaacactt ggccagaaat gcgcaaccat tggagctctc cggattaccc
    10081 aaaggttaac gagtttgaac gcctctgccc actcgcccat ctctgatggt ttcccaagaa
    10141 ctcctcaagc aaaatatata taattgtgtg tattatgcac agacacgaga aaatgctgtt
    10201 tttctgatct gcattacagc acatttgccc gccaacgaca ataccaccca ctcggtacct
    10261 cgctgactcc tgatgcctga tacctgcgcg gtgactgtct acaatctgca taatcaagag
    10321 aagttgtgtt gaagacgagc gccacacaac cgtttccaca aggtcaccca aggccggtgc
    10381 agatgtaggt gaggtctcca taaacagact gaaataaaca catcctccgc tgggaacaac
    10441 aaccccctca cgcctcatgc atttccataa gcctacatgc atctcttcca acttatggag
    10501 actcgcacct accaacatcc gcacaacaaa gatatacaga gcgcgctccc tcaggtcaag
    10561 gccctgtggg ggtctgtgca gaaataggtc atttgtcaca catcaagtcc tggggcagga
    10621 gatgcattat agatgagacc aaacagcctg tctcggtgag ctctacccac tccctgagac
    10681 tagaaatggg ggaagggagc ttgagataac aaccgctgca atcactgtgt cgatgtttaa
    10741 tatcagcacc aaccgggaac aataaggaga tgggtgcatt catgttcaca tcttaccagt
    10801 caagtatcat cgaaccggct tgataaccac acctcgtgta atagctgagc agatagttgt
    10861 cattttaaag cgttggcctt tgtcgattat gtaatgcgca cattcaacac atggtaatat
    10921 agaaacggtt atgtcgaggt tgttttgtcc agagatgacc ttcacacagt tacagccgct
    10981 ctgcatccac acaaatggag gacttaatcg tggactgcat tcttagaaat gatctacaaa
    11041 gacaaataat gtgaaatcaa gaaaggacaa aatttaagta aggggatgag ggagagagag
    11101 aacgaggggc aaggagaaag catggctcct gtctttttct gcacccatct gttcggagtg
    11161 caggtggagc tctattcact cagctctgca tgtgtgtttg gggggggcag gaagaaaggg
    11221 agggcaaaag gaagagtgga gagatggtgg gggctggagg gatggggggt tctcggtgat
    11281 ctctcctgaa ggggataatg ggagagcagc gctttgcaat ggctgccatg tagtaccctc
    11341 cctgcacaat tagccaatca gcagcaagct ctgccagcca gaaggacaca taaaagaaga
    11401 acattgcagc agaggcacag aaggagcctg cgaggagctg ggaaatacac acacaacagc
    11461 agaaccacaa caccctcccc tggacacacc ctactgggga tcactgcttt tctttttttc
    11521 tgaaccatcg cccacgccac acggagagaa atctctctct catcatcatc ctgaagaaaa
    11581 cccccttatc ctcattttca cactgctgag gaaaacctac aatcgcacgg gctgagattt
    11641 cctggcgaag actgtccttt ttcctttttc tttttttttc ttttcctttg gaaactgaca
    11701 tttgcatttc tccattccaa gccacggcgt aataatatct gcaatccagc ctgaagacct
    11761 gcaaatcgaa ggacctagat cactatcatc tttgtacgtc aaggatcctg gttactgtac
    11821 gtataacctt tctttctttt gctctgacca atatgaaaca ctaaaatcga ttcgagcagc
    11881 ctctcagcat caattacagt gcgtgaaaaa cattcaaata gaggcagcaa atattacatg
    11941 tgaaaataca ggctggctaa atccaagcta atttagaaat gtggtcaaaa cgcatactgg
    12001 cacgtctaat cgcggattca gtaaacaaga ttaacgatta gcccagtgta taagtcatat
    12061 gataccggca tgcgcgagag catcgctaca cccgagctgg cttcattttc ggaggaaaat
    12121 caaaacattg ctttctcctg ccgtgcgaac cattcgtcat aaaccgtaat acgcaacata
    12181 catttattac tacatccgtt aattagcgat aattagccgt tattaacaaa gagcgctgag
    12241 gaattcctct caaatagcgg aggtgcggcg gaggcagagg ggcgtaaaag ggcacatgcg
    12301 tgcctggctc aaaaaaggat gctccagact gaaactcaga gacaaaacac gatgcctcgg
    12361 aggctgagag cccatcgaga ggaaaggcaa agaaaagggt cgagtcactc agagggggag
    12421 gggagatatt gtgcatccat tggtttgaat tgaagcaggc agaataaaaa tccttgcgat
    12481 tatgcttcgt gagagatcgc gagagaggaa aaggctaaat gccacgtatg caaatggata
    12541 aatgtcatct atttcttcgc gagtagacat tttgtggaca acgagagtgt aaataagcga
    12601 tatgagggga atacaaaggc cttgagggaa agcgttttgg gcgtgtcttg tcgaaaagaa
    12661 gacagcgatg gcacgcgctc agaaccacca tcttcacgta gacaatccgc gtgaaaacaa
    12721 taacaaaact gctcatttta acacgagcag gtgtttgaaa ccacccaaaa aagattgtct
    12781 aaaatattaa gattaattaa tgacgtttaa atgtccaggc ctgatataaa tgaagcgtgt
    12841 agtaagagta atcgcctttg tttcttttca gagatacgtt ttcagagatt cagggaatta
    12901 tatcttcaat aaaataatgt ttgatttcat tattttaaag ctttttaaga agtaaaaata
    12961 cacattattc atttacattt tattttattt aaaggcctat ggtcttgaaa ataataagtg
    13021 gaatatatag gctatatatt cgtctaagga cagccggtgt tattttatag tgacctcaga
    13081 caaaagccga gacaaaacga ggcgcttgcc tttcatttta atccttaata gacggtgtga
    13141 tgaatgaatg aatgaatgaa taaatgaatg aatgacgggc ccttcggcat gttgttccgc
    13201 agtcctccgt gagcacgagt ctctaccgca gtgccaagca cataaaaccg ttgagtcaga
    13261 taaccttctc caggcgcgta tacacaattc aaggacaagc acatcacaac tatacacaac
    13321 acaacacaac cgcctaaacg cacgagctca tcaatcgcaa tttaaaggca tgtcagtcaa
    13381 agaaaaaggc ggcgttattt aaaaccaatg ttcaacaata tttgtatgct gactagcccg
    13441 ggcgcgcacg ctataagatc acaaggcctt gttttttccc tttattcaaa atatcattaa
    13501 ttattacagg tcataaaaca aaacaatgcg aattacaatg atttaaagac tattgattta
    13561 acaataatca gttaattata gaataatatt ataatatttt acatacaaat gaaagatgac
    13621 attatttgat gacattgtaa ttaaaatata tgcaatatat ttttacgttt ttattaatta
    13681 ggctattact attattataa ttatcattat ttttattatt attattatta ttattaaata
    13741 gtcttgcgaa ataagagtga aggcagttgc aatgttcatt gttcccggag agtcccgcag
    13801 cctctggcct caacacaaag cgctactgtt tacattaata ttcatagtgc tgccatggtc
    13861 tctgtagagg aggaacgggt ggctcttttg ttcgctagtg tcaatacgtt tattcagcaa
    13921 aaagcagctc ggatttgtga atgcccaaca agcacacgcg cactccaatc aaaggacgag
    13981 aggataacga ttttaaacga ttcgcaaacg tgtttatttg taaagacgac atcaagagct
    14041 taatgtccac ttgaaaagaa ataaatatgc ccgcggcgcc aaggatattt tgaatgtagt
    14101 cgttcatggt gagccactcc cgaattcagc actctggaga gcgaccaaag aggaatagcg
    14161 gaatagcaac tacttgagcc atttctctcc gtttgatggc gttcatgatt aaaaatataa
    14221 tcacgtgatt ttttatacat atataaatat atacataaag taacgctttc cttttgagaa
    14281 tacataaata tttattcgcg tgataaatca atgatcttat atttatttgc acgaccgaaa
    14341 ttagccaaat tcaccaatta aaaaaaaaat aagccaacaa aaaagaaggg tttatccgtt
    14401 caattttgac ttgtgtcctg ttgttttaca gctgccactg tgatccctta agctgcagtg
    14461 agagtgtggc taatgccttt tgtttaagat aatcctgtaa tctgttaccg aaacggccta
    14521 ttgacaagcc ggcattcaca tttcagtcag gaggcccgtc cagacatgta catttatgaa
    14581 ttacgaatga taaaattaag atctgcatta ggcgtcagaa atgtcacgaa cacccattca
    14641 tttcccacag ccaataatgg atcatgctgg gaagcgctat cctcgcagtc tcaaaattaa
    14701 tgttgacatg ttgcgtagag ctacagatta gatcagccat atgctttatg tgtttacctc
    14761 agcggatctt cagcaagatg tgtttatttt aagaaaatgg cttcctcgct gcctgcacag
    14821 gggcattaag gaagtgacgt gagcgtcctc acagtca
//
