LOCUS       sequence-388351-         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of p53 TET-CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     Mapping Interactions of the Intrinsically Disordered C-Terminal 
            Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.
  JOURNAL   Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 
            10.1021/acs.biochem.2c00528. Epub 2022 Nov 15.
  PUBMED    36380579
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.2c00528"; journalName: "Biochemistry"; date: 
            "2022-12-6- 6"; volume: "61"; issue: "23"; pages: "2709-2719"
COMMENT     Sequence Label: H6GB1tevP53(304-393)
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..240
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             265..285
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tagaaataat tttgtttaac tttaataagg agatatacca tgggccatca tcatcatcat
       61 cacagcagcg gcatgactta caaactgatc cttaacggta aaactttgaa aggcgaaacc
      121 actaccgaag ctgttgatgc tgcgactgca gaaaaagttt tcaaacagta cgctaacgac
      181 aacggtgttg acggtgaatg gacttacgac gatgcgacta agaccttcac cgttactgaa
      241 gaattcagca gcggcagcag cggcgaaaac ctgtattttc agggctccca tactaagcga
      301 gcactgccca acaacaccag ctcctctccc cagccaaaga agaaaccact ggatggagaa
      361 tatttcaccc ttcagatccg tgggcgtgag cgcttcgaga tgttccgaga gctgaatgag
      421 gccttggaac tcaaggatgc ccaggctggg aaggagccag gggggagcag ggctcactcc
      481 agccacctga agtccaaaaa gggtcagtct acctcccgcc ataaaaaact catgttcaag
      541 acagaagggc ctgactcaga ctaatagaag cttgcggccg cac
//
