LOCUS       sequence-388355-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of P53 TETCTD for segmental labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     Mapping Interactions of the Intrinsically Disordered C-Terminal 
            Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.
  JOURNAL   Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 
            10.1021/acs.biochem.2c00528. Epub 2022 Nov 15.
  PUBMED    36380579
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.2c00528"; journalName: "Biochemistry"; date: 
            "2022-12-6- 6"; volume: "61"; issue: "23"; pages: "2709-2719"
COMMENT     Sequence Label: H6GB1tev-IntC-P53(304-393)
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..236
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             261..281
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg ccatcatcat catcatcaca
       61 gcagcggcat gacttacaaa ctgatcctta acggtaaaac tttgaaaggc gaaaccacta
      121 ccgaagctgt tgatgctgcg actgcagaaa aagttttcaa acagtacgct aacgacaacg
      181 gtgttgacgg tgaatggact tacgacgatg cgactaagac cttcaccgtt actgaagaat
      241 tcagcagcgg cagcagcggc gaaaacctgt attttcaggg ctcccatatg gaccataatt
      301 ttgcactcaa aaatggcttc atagcttcta attgtttcaa tggtactaag cgagcactgc
      361 ccaacaacac cagctcctct ccccagccaa agaagaaacc actggatgga gaatatttca
      421 cccttcagat ccgtgggcgt gagcgcttcg agatgttccg agagctgaat gaggccttgg
      481 aactcaagga tgcccaggct gggaaggagc caggggggag cagggctcac tccagccacc
      541 tgaagtccaa aaagggtcag tctacctccc gccataaaaa actcatgttc aagacagaag
      601 ggcctgactc agactaatag aagcttgcgg ccgcac
//
