LOCUS       sequence-388356-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of P53 TET-CTD for segmental labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     Mapping Interactions of the Intrinsically Disordered C-Terminal 
            Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.
  JOURNAL   Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 
            10.1021/acs.biochem.2c00528. Epub 2022 Nov 15.
  PUBMED    36380579
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.2c00528"; journalName: "Biochemistry"; date: 
            "2022-12-6- 6"; volume: "61"; issue: "23"; pages: "2709-2719"
COMMENT     Sequence Label: H6SUMO-IntC-P53(304-393)
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctagaatatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagcgaggt caagccagaa gtcaagcctg
      121 agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc aagatcaaaa
      181 agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt aaggaaatgg
      241 actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc cctgaagatt
      301 tggacatgga ggataacgat attattgagg ctcacagaga acagattggt ggtgaccata
      361 attttgcact caaaaatggc ttcatagctt ctaattgttt caatggtacc aagcgagcac
      421 tgcccaacaa caccagctcc tctccccagc caaagaagaa accactggat ggagaatatt
      481 tcacccttca gatccgtggg cgtgagcgct tcgagatgtt ccgagagctg aatgaggcct
      541 tggaactcaa ggatgcccag gctgggaagg agccaggggg gagcagggct cactccagcc
      601 acctgaagtc caaaaagggt cagtctacct cccgccataa aaaactcatg ttcaagacag
      661 aagggcctga ctcagactaa tgcggccgca c
//
