LOCUS       sequence-388357-       14908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HuC driven expression of the consitutively active calcium recorder 
            positive control Caprola_on fused to mEGFP for neuronal expression 
            in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14908)
  AUTHORS   Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N,
            Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, 
            Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
  TITLE     Recording physiological history of cells with chemical labeling.
  JOURNAL   Science. 2024 Feb 23;383(6685):890-897. doi: 
            10.1126/science.adg0812. Epub 2024 Feb 22.
  PUBMED    38386755
REFERENCE   2  (bases 1 to 14908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg0812"; journalName: "Science"; date: 
            "2024-02-23- 23"; volume: "383"; issue: "6685"; pages: "890-897"
COMMENT     Sequence Label: pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
FEATURES             Location/Qualifiers
     source          1..14908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    45..179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(69..88)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     123..142
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(618..805,806..817)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(851..867)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(851..867)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(904..924)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(904..922)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(943..959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(943..959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(956..978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    967..983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(991..997,998..1015,1016..1021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1036..1057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1174..1191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1345..1933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1425..1444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2104..2895,2896..2964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2727..2746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2965..3069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3232..3253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(3602..3613,3614..3776)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             12635..13075
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             join(14192..14194,14195..14197,14198..14908,1..3)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14237..14258)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(14498..14517)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14845..14866
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taaccggtgg atcctggccg tcgttttact gtaccatcga tgatgatcca gacatgataa
       61 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      121 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      181 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
      241 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatct
      301 gcgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc
      361 agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc
      421 cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc
      481 caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc
      541 agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag
      601 caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa
      661 ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt
      721 attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag
      781 taatcaagta aaattactca agtactttac acctctggtt cttgaccccc taccttcagc
      841 aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
      901 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaccccc ccgttcagcc
     1621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1981 aaactcacgt taaggcattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3061 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     3121 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3181 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3241 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3301 cagggcgatg gcccacgctg gctaagaact catcagcctc cccggtccat ctacccacgt
     3361 accaatgcac caattggcca caatgacggc tactacatgg tgccattcct tcctctttat
     3421 aggaatggag actacctcct gtccaacaat gctcttggat acgagtacgc ctacctgttg
     3481 gacccaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct
     3541 gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg
     3601 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
     3661 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
     3721 aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa
     3781 aactagagat tcttgtttaa gcttcccggg tgtcgacgaa ttctcgagct ctattcctaa
     3841 agaccttggg tgaccaaaat cttattttaa taaataaaac tgtttattaa aacttttttg
     3901 tttcaaagaa ccatatgtat agtgaaattt ataaaaatat caatttttaa aaagctggtg
     3961 tactcattta tgttatgaac tctaaaacca tatactgact gcaagtgatg atgtatagag
     4021 tgatgtttac gagtaaacat atttagttgt atacatccta ctgagcacat tttgatgtat
     4081 gaaataacat tacaagcttt atccaaatta agccatttta aaacactgcc aattgaaaat
     4141 acaaatcctg gaaaaaatcg tctttagcgc agtcatttga gccatcctaa tccgttacct
     4201 cagaccataa taagaaggga taacactagc tgtagcaatg gaacacatct gtttcacaca
     4261 atcatatctc ctgcgccggt gctaagcaga ttcagcgtga tcataacatg ctttccactc
     4321 ataaatgtaa atttacaatt tgcacatgta aaacagacac ttttgagata ttggataaaa
     4381 aaacaagagt atattgctta gtttcatcca ccagtcatcc ccacagcgtt tggaaggcca
     4441 taaaaagtgt ctaaaatcaa tgatcattga aagagcacaa gagagactct tacgctgtaa
     4501 tgccactggg gacaaaagtg acagtctctt aatgggctct tctggagggg ctcctgaaca
     4561 ttaaaaatta tcagcgaaat taccgaaaga gcttcaagca actggcatgc ttgatcctct
     4621 gcgtcggggc ggtgaatagg tgcttcagat gccctcttac ccacgggctg gattcagctg
     4681 ccccgctacc agcggagacc ccctaatgag cctctgcaat taagtttatt catgttaagt
     4741 gtgaacgggg tgcgtgcgga actgtgggca gctaacagac ctgggttctt tgtgccacaa
     4801 gtgctgcctt tattcggctc acaaagcaga aaacaacacc cgcacctata atggcgccct
     4861 cggctgggtc taagaaacgt ggcgagttga cagagcagag tgggcggggt taagacagac
     4921 tgacagcggg acccatctcc atcctcttat taacgcttaa cgagtgcctt cctcatgcaa
     4981 tattcatcgc cactaatatc atccaagctc tgagctgagc tggccactta tgtaaggcaa
     5041 ttatgtaaaa tatcagacag ggcccacact cagaatctga ctggggtaga gacgcgggac
     5101 gagaaccgag agcaagaact gaaagtgaaa gtgaccacta aagggaggag aggacagagg
     5161 ggcaggatgt gtcaagatta ccagagaaca cttggccaga aatgcgcaac cattggagct
     5221 ctccggatta cccaaaggtt aacgagtttg aacgcctctg cccactcgcc catctctgat
     5281 ggtttcccaa gaactcctca agcaaaatat atataattgt gtgtattatg cacagacacg
     5341 agaaaatgct gtttttctga tctgcattac agcacatttg cccgccaacg acaataccac
     5401 ccactcggta cctcgctgac tcctgatgcc tgatacctgc gcggtgactg tctacaatct
     5461 gcataatcaa gagaagttgt gttgaagacg agcgccacac aaccgtttcc acaaggtcac
     5521 ccaaggccgg tgcagatgta ggtgaggtct ccataaacag actgaaataa acacatcctc
     5581 cgctgggaac aacaaccccc tcacgcctca tgcatttcca taagcctaca tgcatctctt
     5641 ccaacttatg gagactcgca cctaccaaca tccgcacaac aaagatatac agagcgcgct
     5701 ccctcaggtc aaggccctgt gggggtctgt gcagaaatag gtcatttgtc acacatcaag
     5761 tcctggggca ggagatgcat tatagatgag accaaacagc ctgtctcggt gagctctacc
     5821 cactccctga gactagaaat gggggaaggg agcttgagat aacaaccgct gcaatcactg
     5881 tgtcgatgtt taatatcagc accaaccggg aacaataagg agatgggtgc attcatgttc
     5941 acatcttacc agtcaagtat catcgaaccg gcttgataac cacacctcgt gtaatagctg
     6001 agcagatagt tgtcatttta aagcgttggc ctttgtcgat tatgtaatgc gcacattcaa
     6061 cacatggtaa tatagaaacg gttatgtcga ggttgttttg tccagagatg accttcacac
     6121 agttacagcc gctctgcatc cacacaaatg gaggacttaa tcgtggactg cattcttaga
     6181 aatgatctac aaagacaaat aatgtgaaat caagaaagga caaaatttaa gtaaggggat
     6241 gagggagaga gagaacgagg ggcaaggaga aagcatggct cctgtctttt tctgcaccca
     6301 tctgttcgga gtgcaggtgg agctctattc actcagctct gcatgtgtgt ttgggggggg
     6361 caggaagaaa gggagggcaa aaggaagagt ggagagatgg tgggggctgg agggatgggg
     6421 ggttctcggt gatctctcct gaaggggata atgggagagc agcgctttgc aatggctgcc
     6481 atgtagtacc ctccctgcac aattagccaa tcagcagcaa gctctgccag ccagaaggac
     6541 acataaaaga agaacattgc agcagaggca cagaaggagc ctgcgaggag ctgggaaata
     6601 cacacacaac agcagaacca caacaccctc ccctggacac accctactgg ggatcactgc
     6661 ttttcttttt ttctgaacca tcgcccacgc cacacggaga gaaatctctc tctcatcatc
     6721 atcctgaaga aaaccccctt atcctcattt tcacactgct gaggaaaacc tacaatcgca
     6781 cgggctgaga tttcctggcg aagactgtcc tttttccttt ttcttttttt ttcttttcct
     6841 ttggaaactg acatttgcat ttctccattc caagccacgg cgtaataata tctgcaatcc
     6901 agcctgaaga cctgcaaatc gaaggaccta gatcactatc atctttgtac gtcaaggatc
     6961 ctggttactg tacgtataac ctttctttct tttgctctga ccaatatgaa acactaaaat
     7021 cgattcgagc agcctctcag catcaattac agtgcgtgaa aaacattcaa atagaggcag
     7081 caaatattac atgtgaaaat acaggctggc taaatccaag ctaatttaga aatgtggtca
     7141 aaacgcatac tggcacgtct aatcgcggat tcagtaaaca agattaacga ttagcccagt
     7201 gtataagtca tatgataccg gcatgcgcga gagcatcgct acacccgagc tggcttcatt
     7261 ttcggaggaa aatcaaaaca ttgctttctc ctgccgtgcg aaccattcgt cataaaccgt
     7321 aatacgcaac atacatttat tactacatcc gttaattagc gataattagc cgttattaac
     7381 aaagagcgct gaggaattcc tctcaaatag cggaggtgcg gcggaggcag aggggcgtaa
     7441 aagggcacat gcgtgcctgg ctcaaaaaag gatgctccag actgaaactc agagacaaaa
     7501 cacgatgcct cggaggctga gagcccatcg agaggaaagg caaagaaaag ggtcgagtca
     7561 ctcagagggg gaggggagat attgtgcatc cattggtttg aattgaagca ggcagaataa
     7621 aaatccttgc gattatgctt cgtgagagat cgcgagagag gaaaaggcta aatgccacgt
     7681 atgcaaatgg ataaatgtca tctatttctt cgcgagtaga cattttgtgg acaacgagag
     7741 tgtaaataag cgatatgagg ggaatacaaa ggccttgagg gaaagcgttt tgggcgtgtc
     7801 ttgtcgaaaa gaagacagcg atggcacgcg ctcagaacca ccatcttcac gtagacaatc
     7861 cgcgtgaaaa caataacaaa actgctcatt ttaacacgag caggtgtttg aaaccaccca
     7921 aaaaagattg tctaaaatat taagattaat taatgacgtt taaatgtcca ggcctgatat
     7981 aaatgaagcg tgtagtaaga gtaatcgcct ttgtttcttt tcagagatac gttttcagag
     8041 attcagggaa ttatatcttc aataaaataa tgtttgattt cattatttta aagcttttta
     8101 agaagtaaaa atacacatta ttcatttaca ttttatttta tttaaaggcc tatggtcttg
     8161 aaaataataa gtggaatata taggctatat attcgtctaa ggacagccgg tgttatttta
     8221 tagtgacctc agacaaaagc cgagacaaaa cgaggcgctt gcctttcatt ttaatcctta
     8281 atagacggtg tgatgaatga atgaatgaat gaataaatga atgaatgacg ggcccttcgg
     8341 catgttgttc cgcagtcctc cgtgagcacg agtctctacc gcagtgccaa gcacataaaa
     8401 ccgttgagtc agataacctt ctccaggcgc gtatacacaa ttcaaggaca agcacatcac
     8461 aactatacac aacacaacac aaccgcctaa acgcacgagc tcatcaatcg caatttaaag
     8521 gcatgtcagt caaagaaaaa ggcggcgtta tttaaaacca atgttcaaca atatttgtat
     8581 gctgactagc ccgggcgcgc acgctataag atcacaaggc cttgtttttt ccctttattc
     8641 aaaatatcat taattattac aggtcataaa acaaaacaat gcgaattaca atgatttaaa
     8701 gactattgat ttaacaataa tcagttaatt aatattttac atacaaatga aagatgacat
     8761 tatttgatga cattgtaatt aaaatatatg caatatattt ttacgttttt attaattagg
     8821 ctattactat tattataatt atcattattt ttattattat tattattatt attaaatagt
     8881 cttgcgaaat aagagtgaag gcagttgcaa tgttcattgt tcccggagag tcccgcagcc
     8941 tctggcctca acacaaagcg ctactgttta cattaatatt catagtgctg ccatggtctc
     9001 tgtagaggag gaacgggtgg ctcttttgtt cgctagtgtc aatacgttta ttcagcaaaa
     9061 agcagctcgg atttgtgaat gcccaacaag cacacgcgca ctccaatcaa aggacgagag
     9121 gataacgatt ttaaacgatt cgcaaacgtg tttatttgta aagacgacat caagagctta
     9181 atgtccactt gaaaagaaat aaatatgccc gcggcgccaa ggatattttg aatgtagtcg
     9241 ttcatggtga gccactcccg aattcagcac tctggagagc gaccaaagag gaatagcgga
     9301 atagcaacta cttgagccat ttctctccgt ttgatggcgt tcatgattaa aaatataatc
     9361 acgtgatttt ttatacatat ataaatatat acataaagta acgctttcct tttgagaata
     9421 cataaatatt tattcgcgtg ataaatcaat gatcttatat ttatttgcac gaccgaaatt
     9481 agccaaattc accaattaaa aaaaaaataa gccaacaaaa aagaagggtt tatccgttca
     9541 attttgactt gtgtcctgtt gttttacagc tgccactgtg atcccttaag ctgcagtgag
     9601 agtgtggcta atgccttttg tttaagataa tcctgtaatc tgttaccgaa acggcctatt
     9661 gacaagccgg cattcacatt tcagtcagga ggcccgtcca gacatgtaca tttatgaatt
     9721 acgaatgata aaattaagat ctgcattagg cgtcagaaat gtcacgaaca cccattcatt
     9781 tcccacagcc aataatggat catgctggga agcgctatcc tcgcagtctc aaaattaatg
     9841 ttgacatgtt gcgtagagct acagattaga tcagccatat gctttatgtg tttacctcag
     9901 cggatcttca gcaagatgtg tttattttaa gaaaatggct tcctcgctgc ctgcacaggg
     9961 gcattaagga agtgacgtga gcgtcctcac agtcaaatta ctttatctca tgctgctttc
    10021 aggcctctgc ttattttatt attattattt taaattaggg ggaatcacgg tggcgttgtg
    10081 ggtagggtag cgcgatcacc ttacagcaag aaggtcgctg gttcgagctc tggctgggtc
    10141 agttggcatt tctgtgtgga gtttaaattg aattgaaatt gaataaacta aattggccct
    10201 agtgtatgtg tgtgaatgta agtgtgcatg ggtgtttccc agtgttgggt ttgactggaa
    10261 gagcatccgc ttcgtaaaac atatgctgga taagttggcg gttcatttca ctgtggtgac
    10321 cgctgattaa taaagggact aagctgaaaa tgaaatgaat taatacattt taaatgagct
    10381 tttgctgaca tgtttatgat tacaaatagt gaaattgtat gttaatttac gataagtgca
    10441 ttgttctaaa ttcattttta tgtagcagct ggtttttctt ccatttgtgt cattcaacag
    10501 aacatttttc taaaaaagtg aacttaaatt ttctcctccc tctgacaagg cttgatggta
    10561 atttcagccg actctaatta cagactcact aaaaggaatc ggtgcagtca tttatcttta
    10621 tcagcgcgct ggtgggaagg taataggttt tgcttatgag ttgtttgcgt gcgagtctgg
    10681 gtccttgtgt aggcgatgct gatgcactgc attaaatatt gagggagaag tcgctcaaaa
    10741 atagcaaagt acaggctggg gcaggaacgt caaagcccgc tcagtggcca cgtttcttgt
    10801 ttcgtaagtc ctatttttaa tagcgataat tcaggctcag tagaaggaac tccagcacta
    10861 taagcagtct ctcgcctgtc tcggcgagtt atgtattttg ggatgaagtg ctcttccacc
    10921 tctacctctg ctgtgctggc gccagtatct gggctgtaag atagtgtacg tgtcagtctg
    10981 tgtggccgaa gagcctgtgt gttgtgtaac tcactacatt cgtcgctgtg gatggctgac
    11041 ttgccatggg gaggtttact tcagttcacg aatcacattc gccacgatct tgtcactttg
    11101 tgtcaagact cgctctctgt gtgtcaacag ctctgctgtt tgaggttgaa attctgaact
    11161 tgagatatat cccaggatat attagggcta ctgccaacgg aaaaagcaaa tggtgccatg
    11221 gccatttgat tgatcaaaca aacacccttt tgcttgggtg acagtgaaat gtcagatcca
    11281 tgtttgtact gctttaataa cttgcctctc tgagctttct ccttgtcagt gtcaatttgc
    11341 ctgagatata aattccggtt gccccgaaag cttattttat taaaaaaaag ctaacatgca
    11401 ctcatacaac gtgcgcacat acatgtgagg cttgctaaaa gtcaagaagc aagccaatag
    11461 caagagtcgt cagactgctg ttacctgggc aacctggtta gaggtaaggc tcaaggcaag
    11521 aataaatcat tccactgaag agacacagcg ttacagcgag atgagagcaa gaggcgatag
    11581 tgaagggaga acaaaagaac agcctaccca cactagtgca gatctacctg tttgagctac
    11641 tgattgttca gtttgattag atatcagaca attctgctat ctccaattgc attgctgcat
    11701 gaattcctag aaatcatttt aatcaccaaa aactagtagg gacacttgtg gcgtatatga
    11761 ttctgcctgt tgattgtggg gatccaacag aaagatcatg aatgcattgt taattaagtc
    11821 ggtgagacac cctgttccac cctaggggcc gttctgggga aagagtgctt tcagtcagct
    11881 aaagatgact aatatgtgaa catatattac tacacatgcc aatttgtaca ttctggatag
    11941 ttaccagccc tgggaaaaca tcgaaaaaaa taaatcaaca tagataaaat tggcaaatcc
    12001 cttctggtac gatgcagagt gcttgctctg agaacttgtg cattgtgaca gaaagcgacc
    12061 gttaaaaact gaaagtagca tctacaacct gcatacatct gttctttgaa aatcccctgt
    12121 aggccaaatt aagcatcacg ctgtgctcca cagcaacacg gatgaaccga aaacccagac
    12181 acaaacgcac acacttttca cataaacaag gtttagttat gctagctatg ctttttcgtc
    12241 ctccctttgt catcagcggc gagtgacgta acacagtttg accctggaca gcgtaaaggg
    12301 ggggttggga atgagtgaaa ggactgtgac atctgcaggg ccaggggctg atggagagag
    12361 ctgaagtgag ggaggtcaga ggaggaggaa gggtgggata ttgtcctcct caggccctat
    12421 ccctctcaga cgcattgatt tttaacctct cagcgagaat gccaagcatt acacccctca
    12481 aattatggtt cctcacccaa cctgttatat tttccacctg cagataatta ccggtatgct
    12541 gcagaacgag cttgctctta agttggctgg acttgatatt aacaagactg gaggttctgc
    12601 ccgcgagacc ttccaggcct tccgcaccgg ttctgaccaa ctgactgaag agcagatcgc
    12661 agaatttaaa gaggctttct ccctatttga caaggacggg gatgggacaa taacaaccaa
    12721 ggagctgggg acggtgatgc ggtctctggg gcagaacccc acagaagcag agctgcagga
    12781 catgatcaat gaagtagatg ccgacggtga cggcacaatc gacttccctg agttcctgac
    12841 aatgatggca agaaaaatga aagacacaga cagtgaagaa gaaattagag aagcgttccg
    12901 tgtgtttgat aaggatggca atggctacat cagtgcagca gagcttcgcc acgtgatgac
    12961 aaaccttgga gagaagttaa cagatgaaga ggttgatgaa atgatcaggg aagcagacat
    13021 cgatggggat ggtcaggtaa actacgaaga gtttgtagtg atgatgacag cgaaggaatt
    13081 ccctcctcct ccgccaccgc caccaccgcc gccgccgcca ccaccacctc ctccgcctcc
    13141 tccaccacca ccgccaccgc ctccaccacc tggcggcagc agggtcgact catcacgtcg
    13201 taagttcaat aagacaggtc acgcactgag agctataggt cggctgagct cactcgaggg
    13261 cggttctttt gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct
    13321 gatcatcgat cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct
    13381 gactgaagtc gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc
    13441 actgtggcgc ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct
    13501 ggtcgaagaa tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg
    13561 caccccaggc gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa
    13621 ctgcaaggct gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct
    13681 gatcggcagc gagatcgcgc gctggctgtc tactctggag attggtggca ccggtggctc
    13741 tggcggtacc ggtggctctg gcggttctat cggtactggc tttccattcg acccccatta
    13801 tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc
    13861 tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca
    13921 tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa
    13981 accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc
    14041 cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca
    14101 ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc
    14161 tatcccgacc tgggacgaat ggggaggttc tatggtgagc aagggcgagg agctgttcac
    14221 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
    14281 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
    14341 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
    14401 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
    14461 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
    14521 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
    14581 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
    14641 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
    14701 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
    14761 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca aactgagcaa
    14821 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
    14881 cactctcgga atggacgagc tgtacaag
//
