LOCUS       sequence-388364-       10014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV driven expression of the inactive calcium recorder negative 
            control Caprola_off fused to mEGFP for neuronal expression through 
            lentivirus transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N,
            Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, 
            Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
  TITLE     Recording physiological history of cells with chemical labeling.
  JOURNAL   Science. 2024 Feb 23;383(6685):890-897. doi: 
            10.1126/science.adg0812. Epub 2024 Feb 22.
  PUBMED    38386755
REFERENCE   2  (bases 1 to 10014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg0812"; journalName: "Science"; date: 
            "2024-02-23- 23"; volume: "383"; issue: "6685"; pages: "890-897"
COMMENT     Sequence Label: pLKO.1-puro_CMV_NES-Caprola_off-mEGFP
FEATURES             Location/Qualifiers
     source          1..10014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    127..148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(163..168,169..186,187..193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    201..217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     206..228
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     225..241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     225..241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     260..280
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        262..280
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        308..534
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             535..715
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    762..887
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1380..1613
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1798..1842
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2140..2388
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2140..2160
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2311..2330
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        2509..2812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2813..3016
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2967..2987
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3130..3570
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             3691..3747
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHALRAIGRLSS"
     CDS             join(4651..4653,4654..4656,4657..5370)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4696..4717)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4957..4976)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5304..5325
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5415..5532
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5581..6091
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6010..6028
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6113..6712
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6113..6132)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6609..6629
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6840..7073
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7145..7266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7182..7201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7236..7255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7306..7441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7461..7480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7462..7480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7490..7507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7490..7506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7499..7521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7648..8103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7735..7754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7945..7966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8129..8233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8234..8302,8303..9094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8452..8471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9265..9853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9754..9773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(10007..10014,1..10)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
       61 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      121 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      181 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      241 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
      301 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
      361 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      421 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      481 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      541 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      601 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      661 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      721 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      781 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      841 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      901 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      961 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1021 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1081 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1141 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1201 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1261 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1321 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1381 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1441 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1501 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1561 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1621 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1681 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1741 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1801 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1861 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1921 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1981 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2041 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     2101 cgatcacgag actagcctcg agcggccgcc cccttcaccg agggcctatt tcccatgatt
     2161 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
     2221 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     2281 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     2341 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gcgcgatagc
     2401 gctaataatt tctcgagaaa ttattagcgc tatcgcgctt tttgaattca gttattaata
     2461 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2521 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2581 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2641 tttacggtaa gctgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2701 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2761 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2821 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2881 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2941 atgtcgtaag aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3001 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
     3061 atgctgcaga acgagcttgc tcttaagttg gctggacttg atattaacaa gactggaggt
     3121 tctctgccgg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
     3181 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     3241 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     3301 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaagac
     3361 acagacagtg aagaagaaat tagagaagcg ttccgtgtgt ttgataagga tggcaatggc
     3421 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     3481 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     3541 gaagagtttg tagtgatgat gacagcgaag gaattccctc ctcctccgcc accgccacca
     3601 ccgccgccgc cgccaccacc acctcctccg cctcctccac caccaccgcc accgcctcca
     3661 ccacctggcg gcagcagggt cgactcatca cgtcgtaagt ggaataagac aggtcacgca
     3721 ctgagagcta taggtcggct gagctcactc gagggcggtt ctgacgtcgg ccgcaagctg
     3781 atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg
     3841 actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca
     3901 ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg
     3961 gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc
     4021 accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac
     4081 tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg
     4141 atcggcagcg agatcgcgcg ctggctgtct actctggaga ttggtggcac cggtggctct
     4201 ggcggtaccg gtggctctgg cggttctatc ggtactggct ttccattcga cccccattat
     4261 gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct
     4321 gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat
     4381 gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa
     4441 ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc
     4501 ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac
     4561 tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct
     4621 atcccgacct gggacgaatg gggaggttct atggtgagca agggcgagga gctgttcacc
     4681 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4741 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4801 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4861 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4921 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4981 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5041 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     5101 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     5161 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5221 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa actgagcaaa
     5281 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5341 actctcggca tggacgagct gtacaagtaa aattaattct cgacctcgag acaaatggca
     5401 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     5461 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     5521 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
     5581 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     5641 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     5701 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     5761 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     5821 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     5881 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     5941 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     6001 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     6061 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
     6121 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6181 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6241 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6301 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6361 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6421 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6481 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6541 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6601 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6661 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6721 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag
     6781 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6841 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6901 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6961 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7021 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7081 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7141 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7261 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7441 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7501 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7561 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7621 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8041 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8101 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8221 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8281 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8341 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8401 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8461 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8521 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8581 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8641 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8701 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8761 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8821 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8881 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8941 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9001 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9061 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9121 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9901 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9961 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtc
//
