LOCUS       sequence-388365-       10080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV driven expression of the consitutively active calcium recorder 
            positive control Caprola_on fused to mEGFP for neuronal expression 
            through lentivirus transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N,
            Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, 
            Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
  TITLE     Recording physiological history of cells with chemical labeling.
  JOURNAL   Science. 2024 Feb 23;383(6685):890-897. doi: 
            10.1126/science.adg0812. Epub 2024 Feb 22.
  PUBMED    38386755
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg0812"; journalName: "Science"; date: 
            "2024-02-23- 23"; volume: "383"; issue: "6685"; pages: "890-897"
COMMENT     Sequence Label: pLKO.1-puro_CMV_NES-Caprola_on-mEGFP
FEATURES             Location/Qualifiers
     source          1..10080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    87..108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(123..128,129..146,147..153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     185..201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     185..201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     220..240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        222..240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        268..494
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             495..675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    722..847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1340..1573
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1758..1802
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2100..2348
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2100..2120
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        2469..2772
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2773..2976
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2927..2947
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3120..3560
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             join(4677..4679,4680..4682,4683..5396)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4722..4743)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4983..5002)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5330..5351
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5441..5558
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5607..6117
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6036..6054
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6139..6738
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6139..6158)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6635..6655
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6866..7099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7171..7292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7208..7227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7262..7281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7332..7467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7487..7506)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7488..7506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7516..7533)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7516..7532)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7525..7547)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7674..8129
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7761..7780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7971..7992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8155..8259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8260..8328,8329..9120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8478..8497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9291..9879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9780..9799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10033..10050
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      241 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
      301 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
      361 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
      421 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
      481 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      661 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      721 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      781 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      841 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      901 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
      961 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1021 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1081 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1141 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     1201 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1261 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1321 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1381 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1441 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1501 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1561 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1621 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1681 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1741 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1801 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1861 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1921 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     1981 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2041 tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg
     2101 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     2161 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     2221 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactataata
     2281 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2341 gaaacaccgg gcgcgatagc gctaataatt tctcgagaaa ttattagcgc tatcgcgctt
     2401 tttgaattca gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2461 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2521 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2581 tgacgtcaat gggtggagta tttacggtaa gctgcccact tggcagtaca tcaagtgtat
     2641 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2701 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2761 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2821 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2881 aatcaacggg actttccaaa atgtcgtaag aactccgccc cattgacgca aatgggcggt
     2941 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
     3001 tagcgctacc ggtcgccacc atgctgcaga acgagcttgc tcttaagttg gctggacttg
     3061 atattaacaa gactggaggt tctgcccgcg agaccttcca ggccttccgc accggttctg
     3121 accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta tttgacaagg
     3181 acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct ctggggcaga
     3241 accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac ggtgacggca
     3301 caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaagac acagacagtg
     3361 aagaagaaat tagagaagcg ttccgtgtgt ttgataagga tggcaatggc tacatcagtg
     3421 cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat gaagaggttg
     3481 atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac gaagagtttg
     3541 tagtgatgat gacagcgaag gaattccctc ctcctccgcc accgccacca ccgccgccgc
     3601 cgccaccacc acctcctccg cctcctccac caccaccgcc accgcctcca ccacctggcg
     3661 gcagcagggt cgactcatca cgtcgtaagt tcaataagac aggtcacgca ctgagagcta
     3721 taggtcggct gagctcactc gagggcggtt cttttgcccg cgagaccttc caggccttcc
     3781 gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc
     3841 tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt
     3901 tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg
     3961 gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc
     4021 ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg
     4081 ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc
     4141 tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtctactc
     4201 tggagattgg tggcaccggt ggctctggcg gtaccggtgg ctctggcggt tctatcggta
     4261 ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg
     4321 atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct
     4381 acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc
     4441 tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc
     4501 gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg
     4561 actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta
     4621 ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatgggga ggttctatgg
     4681 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4741 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4801 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4861 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4921 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4981 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5041 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5101 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5161 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5221 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5281 tgagcaccca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5341 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaaatt
     5401 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5461 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5521 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5581 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
     5641 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     5701 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     5761 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     5821 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     5881 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     5941 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     6001 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     6061 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     6121 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6181 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6241 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6601 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6721 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     6781 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6841 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6901 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7081 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7141 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7321 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7381 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7441 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7501 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7561 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7621 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     7681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7861 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7981 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8101 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8461 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8941 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
//
