LOCUS       sequence-388460-       14888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HuC driven expression of the calcium recorder Caprola_01 fused to 
            mEGFP for neuronal expression in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N,
            Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, 
            Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
  TITLE     Recording physiological history of cells with chemical labeling.
  JOURNAL   Science. 2024 Feb 23;383(6685):890-897. doi: 
            10.1126/science.adg0812. Epub 2024 Feb 22.
  PUBMED    38386755
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg0812"; journalName: "Science"; date: 
            "2024-02-23- 23"; volume: "383"; issue: "6685"; pages: "890-897"
COMMENT     Sequence Label: pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP
FEATURES             Location/Qualifiers
     source          1..14888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    45..179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(69..88)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     123..142
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(618..805,806..817)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(851..867)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(851..867)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(904..924)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(904..922)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(943..959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(943..959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(956..978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    967..983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(991..997,998..1015,1016..1021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1036..1057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1174..1191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1345..1933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1425..1444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2104..2895,2896..2964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2727..2746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2965..3069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3232..3253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(3602..3613,3614..3776)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             12651..13091
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             13212..13268
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHALRAIGRLSS"
     CDS             join(14172..14174,14175..14177,14178..14888,1..3)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14217..14238)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(14478..14497)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14825..14846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taaccggtgg atcctggccg tcgttttact gtaccatcga tgatgatcca gacatgataa
       61 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      121 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      181 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
      241 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatct
      301 gcgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc
      361 agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc
      421 cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc
      481 caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc
      541 agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag
      601 caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa
      661 ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt
      721 attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag
      781 taatcaagta aaattactca agtactttac acctctggtt cttgaccccc taccttcagc
      841 aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
      901 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1981 aaactcacgt taaggcattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3061 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     3121 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3181 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3241 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3301 cagggcgatg gcccacgctg gctaagaact catcagcctc cccggtccat ctacccacgt
     3361 accaatgcac caattggcca caatgacggc tactacatgg tgccattcct tcctctttat
     3421 aggaatggag actacctcct gtccaacaat gctcttggat acgagtacgc ctacctgttg
     3481 gacccaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct
     3541 gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg
     3601 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
     3661 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
     3721 aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa
     3781 aactagagat tcttgtttaa gcttcccggg tgtcgacgaa ttctcgagct ctattcctaa
     3841 agaccttggg tgaccaaaat cttattttaa taaataaaac tgtttattaa aacttttttg
     3901 tttcaaagaa ccatatgtat agtgaaattt ataaaaatat caatttttaa aaagctggtg
     3961 tactcattta tgttatgaac tctaaaacaa tatactgact gcaagtgatg atgtatagag
     4021 tgatgtttac gagtaaacat atttagttgt atacatccta ctgagcacat tttgatgtat
     4081 gaaataacat tacaagcttt atccaaatta agccatttta aaacactgcc aattgaaaat
     4141 acaaatcctg gaaaaaatcg tctttagcgc agtcatttga gccatcctaa tccgttacct
     4201 cagaccataa taagaaggga taacactagc tgtagcaatg gaacacatct gtttcacaca
     4261 atcatatctc ctgcgccggt gctaagcaga ttcagcgtga tcataacatg ctttccactc
     4321 ataaatgtaa atttacaatt tgcacatgta aaacagacac ttttgagata ttggataaaa
     4381 aaacaagagt atattgctta gtttcatcca ccagtcatcc ccacagcgtt tggaaggcca
     4441 taaaaagtgt ctaaaatcaa tgatcattga aagagcacaa gagagactct tacgctgtaa
     4501 tgccactggg gacaaaagtg acagtctctt aatgggctct tctggagggg ctcctgaaca
     4561 ttaaaaatta tcagcgaaat taccgaaaga gcttcaagca actggcatgc ttgatcctct
     4621 gcgtcggggc ggtgaatagg tgcttcagat gccctcttac ccacgggctg gattcagctg
     4681 ccccgctacc agcggagacc ccctaatgag cctctgcaat taagtttatt catgttaagt
     4741 gtgaacgggg tgcgtgcgga actgtgggca gctaacagac ctgggttctt tgtgccacaa
     4801 gtgctgcctt tattcggctc acaaagcaga aaacaacacc cgcacctata atggcgccct
     4861 cggctgggtc taagaaacgt ggcgagttga cagagcagag tgggcggggt taagacagac
     4921 tgacagcggg acccatctcc atcctcttat taacgcttaa cgagtgcctt cctcatgcaa
     4981 tattcatcgc cactaatatc atccaagctc tgagctgagc tggccactta tgtaaggcaa
     5041 ttatgtaaaa tatcagacag ggcccacact cagaatctga ctggggtaga gacgcgggac
     5101 gagaaccgag agcaagaact gaaagtgaaa gtgaccacta aagggaggag aggacagagg
     5161 ggcaggatgt gtcaagatta ccagagaaca cttggccaga aatgcgcaac cattggagct
     5221 ctccggatta cccaaaggtt aacgagtttg aacgcctctg cccactcgcc catctctgat
     5281 ggtttcccaa gaactcctca agcaaaatat atataattgt gtgtattatg cacagacacg
     5341 agaaaatgct gtttttctga tctgcattac agcacatttg cccgccaacg acaataccac
     5401 ccactcggta cctcgctgac tcctgatgcc tgatacctgc gcggtgactg tctacaatct
     5461 gcataatcaa gagaagttgt gttgaagacg agcgccacac aaccgtttcc acaaggtcac
     5521 ccaaggccgg tgcagatgta ggtgaggtct ccataaacag actgaaataa acacatcctc
     5581 cgctgggaac aacaaccccc tcacgcctca tgcatttcca taagcctaca tgcatctctt
     5641 ccaacttatg gagactcgca cctaccaaca tccgcacaac aaagatatac agagcgcgct
     5701 ccctcaggtc aaggccctgt gggggtctgt gcagaaatag gtcatttgtc acacatcaag
     5761 tcctggggca ggagatgcat tatagatgag accaaacagc ctgtctcggt gagctctacc
     5821 cactccctga gactagaaat gggggaaggg agcttgagat aacaaccgct gcaatcactg
     5881 tgtcgatgtt taatatcagc accaaccggg aacaataagg agatgggtgc attcatgttc
     5941 acatcttacc agtcaagtat catcgaaccg gcttgataac cacacctcgt gtaatagctg
     6001 agcagatagt tgtcatttta aagcgttggc ctttgtcgat tatgtaatgc gcacattcaa
     6061 cacatggtaa tatagaaacg gttatgtcga ggttgttttg tccagagatg accttcacac
     6121 agttacagcc gctctgcatc cacacaaatg gaggacttaa tcgtggactg cattcttaga
     6181 aatgatctac aaagacaaat aatgtgaaat caagaaagga caaaatttaa gtaaggggat
     6241 gagggagaga gagaacgggg ggcaaggaga aagcatggct cctgtctttt tctgcaccca
     6301 tctgttcgga gtgcaggtgg agctctattc actcagctct gcatgtgtgt ttgggggggg
     6361 caggaagaaa gggagggcaa aaggaagagt ggagagatgg tgggggctgg agggatgggg
     6421 ggttctcggt gatctctcct gaaggggata atgggagagc agcgctttgc aatggctgcc
     6481 atgtagtacc ctccctgcac aattagccaa tcagcagcaa gctctgccag ccagaaggac
     6541 acataaaaga agaacattgc agcagaggca cagaaggagc ctgcgaggag ctgggaaata
     6601 cacacacaac agcagaacca caacaccctc ccctggacac accctactgg ggatcactgc
     6661 ttttcttttt ttctgaacca tcgcccacgc cacacggaga gaaatctctc tctcatcatc
     6721 atcctgaaga aaaccccctt atcctcattt tcacactgct gaggaaaacc tacaatcgca
     6781 cgggctgaga tttcctggcg aagactgtcc tttttccttt ttcttttttt ttcttttcct
     6841 ttggaaactg acatttgcat ttctccattc caagccacgg cgtaataata tctgcaatcc
     6901 agcctgaaga cctgcaaatc gaaggaccta gatcactatc atctttgtac gtcaaggatc
     6961 ctggttactg tacgtataac ctttctttct tttgctctga ccaatatgaa acactaaaat
     7021 cgattcgagc agcctctcag catcaattac agtgcgtgaa aaacattcaa atagaggcag
     7081 caaatattac atgtgaaaat acaggctggc taaatccaag ctaatttaga aatgtggtca
     7141 aaacgcatac tggcacgtct aatcgcggat tcagtaaaca agattaacga ttagcccagt
     7201 gtataagtca tatgataccg gcatgcgcga gagcatcgct acacccgagc tggcttcatt
     7261 ttcggaggaa aatcaaaaca ttgctttctc ctgccgtgcg aaccattcgt cataaaccgt
     7321 aatacgcaac atacatttat tactacatcc gttaattagc gataattagc cgttattaac
     7381 aaagagcgct gaggaattcc tctcaaatag cggaggtgcg gcggaggcag aggggcgtaa
     7441 aagggcacat gcgtgcctgg ctcaaaaaag gatgctccag actgaaactc agagacaaaa
     7501 cacgatgcct cggaggctga gagcccatcg agaggaaagg caaagaaaag ggtcgagtca
     7561 ctcagagggg gaggggagat attgtgcatc cattggtttg aattgaagca ggcagaataa
     7621 aaatccttgc gattatgctt cgtgagagat cgcgagagag gaaaaggcta aatgccacgt
     7681 atgcaaatgg ataaatgtca tctatttctt cgcgagtaga cattttgtgg acaacgagag
     7741 tgtaaataag cgatatgagg ggaatacaaa ggccttgagg gaaagcgttt tgggcgtgtc
     7801 ttgtcgaaaa gaagacagcg atggcacgcg ctcagaacca ccatcttcac gtagacaatc
     7861 cgcgtgaaaa caataacaaa actgctcatt ttaacacgag caggtgtttg aaaccaccca
     7921 aaaaagattg tctaaaatat taagattaat taatgacgtt taaatgtcca ggcctgatat
     7981 aaatgaagcg tgtagtaaga gtaatcgcct ttgtttcttt tcagagatac gttttcagag
     8041 attcagggaa ttatatcttc aataaaataa tgtttgattt cattatttta aagcttttta
     8101 agaagtaaaa atacacatta ttcatttaca ttttatttta tttaaaggcc tatggtcttg
     8161 aaaataataa gtggaatata taggctatat attcgtctaa ggacagccgg tgttatttta
     8221 tagtgacctc agacaaaagc cgagacaaaa cgaggcgctt gcctttcatt ttaatcctta
     8281 atagacggtg tgatgaatga atgaatgaat gaataaatga atgaatgacg ggcccttcgg
     8341 catgttgttc cgcagtcctc cgtgagcacg agtctctacc gcagtgccaa gcacataaaa
     8401 ccgttgagtc agataacctt ctccaggcgc gtatacacaa ttcaaggaca agcacatcac
     8461 aactatacac aacacaacac aaccgcctaa acgcacgagc tcatcaatcg caatttaaag
     8521 gcatgtcagt caaagaaaaa ggcggcgtta tttaaaacca atgttcaaca atatttgtat
     8581 gctgactagc ccgggcgcgc acgctataag atcacaaggc cttgtttttt ccctttattc
     8641 aaaatatcat taattattac aggtcataaa acaaaacaat gcgaattaca atgatttaaa
     8701 gactattgat ttaacaataa tcagttaatt atagaataat attataatat tttacataca
     8761 aatgaaagat gacattattt gatgacattg taattaaaat atatgcaata tatttttacg
     8821 tttttattaa ttaggctatt actattatta taattatcat tatttttatt attattatta
     8881 ttattattaa atagtcttgc gaaataagag tgaaggcagt tgcaatgttc attgttcccg
     8941 gagagtcccg cagcctctgg cctcaacaca aagcgctact gtttacatta atattcatag
     9001 tgctgccatg gtctctgtag aggaggaacg ggtggctctt ttgttcgcta gtgtcaatac
     9061 gtttattcag caaaaagcag ctcggatttg tgaatgccca acaagcacac gcgcactcca
     9121 atcaaaggac gagaggataa cgattttaaa cgattcgcaa acgtgtttat ttgtaaagac
     9181 gacatcaaga gcttaatgtc cacttgaaaa gaaataaata tgcccgcggc gccaaggata
     9241 ttttgaatgt agtcgttcat ggtgagccac tcccgaattc agcactctgg agagcgacca
     9301 aagaggaata gcggaatagc aactacttga gccatttctc tccgtttgat ggcgttcatg
     9361 attaaaaata taatcacgtg attttttata catatataaa tatatacata aagtaacgct
     9421 ttccttttga gaatacataa atatttattc gcgtgataaa tcaatgatct tatatttatt
     9481 tgcacgaccg aaattagcca aattcaccaa ttaaaaaaaa aataagccaa caaaaaagaa
     9541 gggtttatcc gttcaatttt gacttgtgtc ctgttgtttt acagctgcca ctgtgatccc
     9601 ttaagctgca gtgagagtgt ggctaatgcc ttttgtttaa gataatcctg taatctgtta
     9661 ccgaaacggc ctattgacaa gccggcattc acatttcagt caggaggccc gtccagacat
     9721 gtacatttat gaattacgaa tgataaaatt aagatctgca ttaggcgtca gaaatgtcac
     9781 gaacacccat tcatttccca cagccaataa tggatcatgc tgggaagcgc tatcctcgca
     9841 gtctcaaaat taatgttgac atgttgcgta gagctacaga ttagatcagc catatgcttt
     9901 atgtgtttac ctcagcggat cttcagcaag atgtgtttat tttaagaaaa tggcttcctc
     9961 gctgcctgca caggggcatt aaggaagtga cgtgagcgtc ctcacagtca aattacttta
    10021 tctcatgctg ctttcaggcc tctgcttatt ttattattat tattttaaat tagggggaat
    10081 cacggtggcg ttgtgggtag ggtagcgcga tcaccttaca gcaagaaggt cgctggttcg
    10141 agctctggct gggtcagttg gcatttctgt gtggagttta aattgaattg aaattgaata
    10201 aactaaattg gccctagtgt atgtgtgtga atgtaagtgt gcatgggtgt ttcccagtgt
    10261 tgggtttgac tggaagagca tccgcttcgt aaaacatatg ctggataagt tggcggttca
    10321 tttcactgtg gtgaccgctg attaataaag ggactaagct gaaaatgaaa tgaattaata
    10381 cattttaaat gagcttttgc tgacatgttt atgattacaa atagtgaaat tgtatgttaa
    10441 tttacgataa gtgcattgtt ctaaattcat ttttatgtag cagctggttt ttcttccatt
    10501 tgtgtcattc aacagaacat ttttctaaaa aagtgaactt aaattttctc ctccctctga
    10561 caaggcttga tggtaatttc agccgactct aattacagac tcactaaaag gaatcggtgc
    10621 agtcatttat ctttatcagc gcgctggtgg gaaggtaata ggttttgctt atgagttgtt
    10681 tgcgtgcgag tctgggtcct tgtgtaggcg atgctgatgc actgcattaa atattgaggg
    10741 agaagtcgct caaaaatagc aaagtacagg ctggggcagg aacgtcaaag cccgctcagt
    10801 ggccacgttt cttgtttcgt aagtcctatt tttaatagcg ataattcagg ctcagtagaa
    10861 ggaactccag cactataagc agtctctcgc ctgtctcggc gagttatgta ttttgggatg
    10921 aagtgctctt ccacctctac ctctgctgtg ctggcgccag tatctgggct gtaagatagt
    10981 gtacgtgtca gtctgtgtgg ccgaagagcc tgtgtgttgt gtaactcact acattcgtcg
    11041 ctgtggatgg ctgacttgcc atggggaggt ttacttcagt tcacgaatca cattcgccac
    11101 gatcttgtca ctttgtgtca agactcgctc tctgtgtgtc aacagctctg ctgtttgagg
    11161 ttgaaattct gaacttgaga tatatcccag gatatattag ggctactgcc aacggaaaaa
    11221 gcaaatggtg ccatggccat ttgattgatc aaacaaacac ccttttgctt gggtgacagt
    11281 gaaatgtcag atccatgttt gtactgcttt aataacttgc ctctctgagc tttctccttg
    11341 tcagtgtcaa tttgcctgag atataaattc cggttgcccc gaaagcttat tttattaaaa
    11401 aaaaagctaa catgcactca tacaacgtgc gcacatacat gtgaggcttg ctaaaagtca
    11461 agaagcaagc caatagcaag agtcgtcaga ctgctgttac ctgggcaacc tggttagagg
    11521 taaggctcaa ggcaagaata aatcattcca ctgaagagac acagcgttac agcgagatga
    11581 gagcaagagg cgatagtgaa gggagaacaa aagaacagcc tacccacact agtgcagatc
    11641 tacctgtttg agctactgat tgttcagttt gattagatat cagacaattc tgctatctcc
    11701 aattgcattg ctgcatgaat tcctagaaat cattttaatc accaaaaact agtagggaca
    11761 cttgtggcgt atatgattct gcctgttgat tgtggggatc caacagaaag atcatgaatg
    11821 cattgttaat taagtcggtg agacaccctg ttccacccta ggggccgttc tggggaaaga
    11881 gtgctttcag tcagctaaag atgactaata tgtgaacata tattactaca catgccaatt
    11941 tgtacattct ggatagttac cagccctggg aaaacatcga aaaaaataaa tcaacataga
    12001 taaaattggc aaatcccttc tggtacgatg cagagtgctt gctctgagaa cttgtgcatt
    12061 gtgacagaaa gcgaccgtta aaaactgaaa gtagcatcta caacctgcat acatctgttc
    12121 tttgaaaatc ccctgtaggc caaattaagc atcacgctgt gctccacagc aacacggatg
    12181 aaccgaaaac ccagacacaa acgcacacac ttttcacata aacaaggttt agttatgcta
    12241 gctatgcttt ttcgtcctcc ctttgtcatc agcggcgagt gacgtaacac agtttgaccc
    12301 tggacagcgt aaaggggggg ttgggaatga gtgaaaggac tgtgacatct gcagggccag
    12361 gggctgatgg agagagctga agtgagggag gtcagaggag gaggaagggt gggatattgt
    12421 cctcctcagg ccctatccct ctcagacgca ttgattttta acctctcagc gagaatgcca
    12481 agcattacac ccctcaaatt atggttcctc acccaacctg ttatattttc cacctgcaga
    12541 taattaccgg tatgctgcag aacgagcttg ctcttaagtt ggctggactt gatattaaca
    12601 agactggagg ttctgcccgc gagaccttcc aggccttccg caccggttct gaccaactga
    12661 ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag gacggggatg
    12721 ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag
    12781 aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc acaatcgact
    12841 tccctgagtt cctgacaatg atggcaagaa aaatgaaaga cacagacagt gaagaagaaa
    12901 ttagagaagc gttccgtgtg tttgataagg atggcaatgg ctacatcagt gcagcagagc
    12961 ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt gatgaaatga
    13021 tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt gtagtgatga
    13081 tgacagcgaa ggaattccct cctcctccgc caccgccacc accgccgccg ccgccaccac
    13141 cacctcctcc gcctcctcca ccaccaccgc caccgcctcc accacctggc ggcagcaggg
    13201 tcgactcatc acgtcgtaag tggaataaga caggtcacgc actgagagct ataggtcggc
    13261 tgagctcact cgagggcggt tctgacgtcg gccgcaagct gatcatcgat cagaacgttt
    13321 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
    13381 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
    13441 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
    13501 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
    13561 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
    13621 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
    13681 gctggctgtc tactctggag attggtggca ccggtggctc tggcggtacc ggtggctctg
    13741 gcggttctat cggtactggc tttccattcg acccccatta tgtggaagtc ctgggcgagc
    13801 gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta
    13861 acccgacctc ctcctacgtg tggcgcaaca tcatcccaca tgttgcaccg acccatcgct
    13921 gcattgctcc agacctgatc ggtatgggca aatccgacaa accagacctg ggttatttct
    13981 tcgacgacca cgtccgcttc atggatgcct tcatcgaagc cctgggtctg gaagaggtcg
    14041 tcctggtcat tcacgactgg ggctccgctc tgggtttcca ctgggccaag cgcaatccag
    14101 agcgcgtcaa aggtattgca tttatggagt tcatccgccc tatcccgacc tgggacgaat
    14161 ggggaggttc tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
    14221 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
    14281 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
    14341 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
    14401 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
    14461 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
    14521 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
    14581 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
    14641 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
    14701 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
    14761 ccgacaacca ctacctgagc acccagtcca aactgagcaa agaccccaac gagaagcgcg
    14821 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
    14881 tgtacaag
//
