LOCUS       sequence-388471-        8996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV driven expression of the m3 GPCR recorder in mammalian cell 
            lines fused to mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Huppertz MC, Wilhelm J, Grenier V, Schneider MW, Falt T, Porzberg N,
            Hausmann D, Hoffmann DC, Hai L, Tarnawski M, Pino G, Slanchev K, 
            Kolb I, Acuna C, Fenk LM, Baier H, Hiblot J, Johnsson K
  TITLE     Recording physiological history of cells with chemical labeling.
  JOURNAL   Science. 2024 Feb 23;383(6685):890-897. doi: 
            10.1126/science.adg0812. Epub 2024 Feb 22.
  PUBMED    38386755
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg0812"; journalName: "Science"; date: 
            "2024-02-23- 23"; volume: "383"; issue: "6685"; pages: "890-897"
COMMENT     Sequence Label: pcDNA3.1-CHRM3-cpHalo∆-mEGFP
FEATURES             Location/Qualifiers
     source          1..8996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3830..3832,3833..3835,3836..4549)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3875..3896)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4136..4155)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4483..4504
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4587..4604)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4593..4817
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4863..5291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4950..4969)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5160..5181
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5300..5320)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5305..5634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5485..5620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5547..5566
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5701..6495
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5755..5774)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6365..6384
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6669..6790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6706..6725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6760..6779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6839..6855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6839..6855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6852..6874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6863..6879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6887..6893,6894..6911,6912..6917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6932..6953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7070..7087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7241..7829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7321..7340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8000..8791,8792..8860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8623..8642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8861..8965)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgcgccac catgaagacg atcatcgccc tgagctacat
     1021 cttctgcctg gtattcgccg actacaagga cgatgatgac gccagcatcg atatgacgct
     1081 gcataataat tcaaccacaa gccctctgtt ccctaacatc agtagtagct ggatacactc
     1141 tcctagcgac gccggacttc ctcccggaac cgtgacccac tttgggtcat ataacgtctc
     1201 tcgggccgca ggcaattttt cttccccaga tgggacaacc gacgatccgc tcggcggcca
     1261 cactgtatgg caggtggttt tcatagcctt tctgaccggc attctggctc tggtcacaat
     1321 catcggcaat attctggtaa ttgtgtcctt caaggtaaac aaacaactga agactgtcaa
     1381 taactacttt ctgcttagtt tggcatgcgc cgatctgatt atcggagtga taagtatgaa
     1441 tctcttcact acttacatca ttatgaacag atgggctctg ggtaacttgg cttgcgacct
     1501 gtggctcgca attgactatg tcgcaagcaa cgccagtgtg atgaaccttc tggttattag
     1561 cttcgaccgc tattttagca ttacacgccc tctgacctac agagcgaaac gcactacaaa
     1621 gcgggcagga gttatgatcg gcctggcctg ggtcatttcc ttcgtgctct gggcaccagc
     1681 catcttgttc tggcagtatt tcgtcggtaa gaggacagtc ccaccaggcg agtgcttcat
     1741 ccaatttttg tccgagccaa ccataacatt cggcacagcc atcgcggcat tttacatgcc
     1801 agtgactatc atgaccattc tgtactggag aatctacaaa gaaaccgaaa agcggacaaa
     1861 ggagttggca gggcttcagg catcaggcac agaggctgaa acagaaaact tcgtgcaccc
     1921 aacagggagt tcacgcagtt gcagctccta cgagctgcag cagcagagta tgaagcgctc
     1981 aaacagaaga aagtacggca ggtgccactt ttggtttact acaaagagct ggaagccaag
     2041 ctctgaacag atggatcaag atcatagctc atcagactca tggaataata atgacgccgc
     2101 cgctagtctg gagaacagtg ccagtagcga tgaggaggac atcggtagtg aaaccagggc
     2161 gatatatagt atcgtgctta agctgccagg tcattcaact atactgaatt ctaccaaact
     2221 tcccagtagc gataatcttc aggtgcctga ggaggagctt ggcatggtcg acttggagcg
     2281 caaggcggat aaactgcagg ctcagaagtc agttgacgat ggaggctctt tccctaagtc
     2341 attttccaaa ctcccaatcc agttggagag cgccgtcgac accgcaaaaa cgagcgatgt
     2401 gaattcatcc gttggcaaaa gcacagcaac tttgcctctt tccttcaaag aggccacact
     2461 cgctaagcga ttcgcactca aaacccggtc acagataacc aagcgcaagc gaatgtcact
     2521 cgtgaaagaa aaaaaagcag cacagacatt gagcgccatc ttgctcgctt ttatcataac
     2581 atggacaccc tacaatatca tggtcctcgt gaacactttc tgcgactcct gtattcctaa
     2641 gactttctgg aacctcggct actggctgtg ctatatcaac agtacagtta accctgtgtg
     2701 ctacgctctc tgtaacaaga cctttaggac cacgttcaag atgctcctcc tgtgccagtg
     2761 tgacaaaaag aaacgacgaa agcagcaata tcagcagcga cagagtgtga tctttcataa
     2821 acgggcgcca gaacaagcgc tcatcgatac cggtggacgc accccaccca gcctgggtcc
     2881 ccaagatgag tcctgcacca ccgccagctc ctccctggcc aaggacactt catcgggtgg
     2941 ggacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagggta cgctgccgat
     3001 gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat taccgcgagc cgttcctgaa
     3061 tcctgttgac cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc
     3121 agcgaacatc gtcgcgctgg tcgaagaata catggactgg ctgcaccagt cccctgtccc
     3181 gaagctgctg ttctggggca ccccaggcgt tctgatccca ccggccgaag ccgctcgcct
     3241 ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc ccgggtctga atctgctgca
     3301 agaagacaac ccggacctga tcggcagcga gatcgcgcgc tggctgtcta ctctggagat
     3361 tggtggcacc ggtggctctg gcggtaccgg tggctctggc ggttctatcg gtactggctt
     3421 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     3481 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     3541 gcgcaacatc atcccacatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     3601 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     3661 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     3721 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     3781 tatggagttc atccgcccta tcccgacctg ggacgaatgg ggtggctcta tggtgagcaa
     3841 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3901 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3961 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4021 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4081 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4141 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4201 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4261 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4321 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4381 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4441 ccagtccaaa ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4501 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtagc tcgagtctag
     4561 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4621 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4681 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4741 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4801 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4861 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4921 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4981 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5041 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5101 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5161 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5221 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5281 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5461 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5581 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5641 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5701 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5761 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5821 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5881 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5941 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6001 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6061 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6121 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6181 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6241 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6301 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6361 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6421 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6481 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6541 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6601 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6661 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6781 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6841 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7021 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7081 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7141 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7681 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7861 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7921 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7981 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8041 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8101 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8161 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8221 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8281 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8341 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8401 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8461 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8521 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8581 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8641 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8701 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8761 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8821 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8881 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8941 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
