LOCUS       sequence-388472-        9715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Li W, Maekiniemi A, Sato H, Osman C, Singer RH
  TITLE     An improved imaging system that corrects MS2-induced RNA 
            destabilization.
  JOURNAL   Nat Methods. 2022 Dec;19(12):1558-1562. doi: 
            10.1038/s41592-022-01658-1. Epub 2022 Nov 10.
  PUBMED    36357695
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01658-1"; journalName: "Nat Methods"; date: 
            "2022-12"; volume: "19"; issue: "12"; pages: "1558-1562"
COMMENT     Sequence Label: yWL234 MCP-GFP-PABPC1*
FEATURES             Location/Qualifiers
     source          1..9715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2636..2656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2660..2686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2660..2686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2687..2710
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      3443..3452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3449..3451,3452..3454,3455..4165)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3449..3451,3452..3454,3455..4165)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3494..3515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3755..3774)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4102..4123
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6105..6693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6158..6178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6576..6587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6768..7001
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7120..7138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7206..7310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7311..7379,7380..8171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7529..7548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8342..8930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8831..8850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9084..9101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9341..9362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9377..9382,9383..9400,9401..9407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9415..9431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9420..9442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9439..9455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9439..9455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9505..9524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgcgccacca tgggcccaaa
     2641 aaagaaaaga aaagttggct acccctacga cgtgcccgac tacgccctgg aagttctgtt
     2701 ccaggggccc ctgttattaa ttaacgcttc taactttact cagttcgttc tcgtcgacaa
     2761 tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga tcgctgaatg
     2821 gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc gtcagagctc
     2881 tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct ggcgttcgta
     2941 cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg agcttattgt
     3001 taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcagcaa tcgcagcaaa
     3061 ctccggcatc tacgccatgg ccagcaactt cacccagttc gtgctggtgg acaacggcgg
     3121 caccggcgac gtgaccgtgg cccccagcaa cttcgccaac ggcatcgccg agtggatcag
     3181 cagcaacagc agaagccagg cctacaaggt gacctgcagc gtgagacaga gcagcgccca
     3241 gaacagaaag tacaccatca aggtggaggt gcccaagggc gcctggagaa gctacctgaa
     3301 catggagctg accatcccca tcttcgccac caacagcgac tgcgagctga tcgtgaaggc
     3361 catgcagggc ctgctgaagg acggcaaccc catccccagc gccatcgccg ccaacagcgg
     3421 catctacgcc gacccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3661 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3721 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3781 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3841 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3901 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3961 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4021 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4081 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4141 tctcggcatg gacgagctgt acaagggcac aggtgcatgc atgaacccca gcgcccccag
     4201 ctaccccatg gcctctctgt acgtggggga cctgcacccc gacgtgaccg aggcgatgct
     4261 ctacgagaag ttcagcccgg ccgggcccat cctctccatc cgggtctgca gggacatgat
     4321 cacccgccgc tccttgggct acgcgtacgt gaacttccag cagccggcgg acgcggaacg
     4381 tgctttggac accatgaatt ttgatgttat aaagggcaag ccagtacgca tcatgtggtc
     4441 tcagcgtgat ccatcacttc gcaaaagtgg agtaggcaac atattcatta aaaatttgga
     4501 caaatccatc gacaataaag cactatatga tacgttttct gcgtttggta acatcctttc
     4561 atgtaaggtg gtttgtgatg aaaatggctc caagggctat ggagttgtac actttgaaac
     4621 acaggaagca gctgaaagag ctattgaaaa aatgaatggg atgcttctaa atgatcgtaa
     4681 agtgtttgtt ggacgattta aatctcggaa ggaacgagaa gcagagcttg gagccagggc
     4741 aaaggagttc accaatgttt acatcaagaa ctttggagaa gacatggatg atgagcgcct
     4801 taaggaactc tttggcaagt ttgggcctgc cttaagtgtg aaagtaatga cagatgaaag
     4861 tggaaaatcc aaaggatttg gatttgtaag ctttgaaagg catgaagatg cgcagaaagc
     4921 tgtggatgag atgaatggga aggagctcaa tggaaaacag atttatgttg gacgagctca
     4981 gaaaaaagtg gaacggcaga cggaacttaa gcgcaaattt gagcagatga agcaagatag
     5041 gatcaccaga tatcagggcg tgaaccttta tgtgaaaaat cttgatgacg ggattgatga
     5101 tgagcgtctc cggaaggagt tttctccgtt tggtacaatc accagtgcaa aagtaatgat
     5161 ggagggtggg cgcagcaaag ggtttggtgt tgtatgtttc tcatcccctg aagaagccac
     5221 taaagcagtt acagagatga atggtagaat tgtggccacg aagccactgt atgtagcttt
     5281 agctcagcgc aaagaagagc gccaggctca cctcactaac cagtatatgc agaggatggc
     5341 aagtgtacga gctgtgccca accccgtgat caacccctac cagccagcac ctccttcagg
     5401 ttacttcatg gcagctatcc cacagactca gaaccgtgct gcatactatc ctcctagcca
     5461 aattgctcaa ctaagaccaa gtcctcgctg gactgctcag ggtgccagac ctcatccatt
     5521 ccagaatatg cccggtgcta tccgcccagc tgctcctaga ccaccattta gtacgatgag
     5581 accagcttcc tcacaggttc cacgagtcat gtcaacacag cgtgttgcta acacatcaac
     5641 acagacaatg ggtccacgtc ctgcagctgc tgctgctgca gccactcctg ctgtccgcac
     5701 cgttccccag tataaatatg ctgcgggagt ccgcaatccc cagcaacatc ttaatgcaca
     5761 gccacaagtt accatgcaac agcctgctgt tcatgtgcaa ggtcaagaac ctttaactgc
     5821 ttccatgttg gcatctgcgc ccccgcaaga gcagaagcaa atgttgggtg aacggctgtt
     5881 tcctcttatc caagccatgc acccttctct tgctggtaaa atcactggca tgctgttgga
     5941 gattgataac tcagaattac ttcacatgct cgagtctcca gagtctctcc gctcaaaggt
     6001 tgatgaagct gtagctgtac tacaagccca ccaagcgaaa gaggctgccc agaaagcagt
     6061 gaacagtgcc actggtgttc caactgtcta aatcgataga tcctaatcaa cctctggatt
     6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6481 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6661 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta
     6721 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6781 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6841 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6901 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6961 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7021 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt
     7081 aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     7141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     7561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8461 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9121 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
     9181 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9241 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
     9301 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9361 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9421 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
     9481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9541 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9601 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     9661 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
