LOCUS       sequence-388530-        5433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Kv1.1 potassium channel 
            subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5433)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Kv1.1/RBG4
FEATURES             Location/Qualifiers
     source          1..5433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     189..208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     442..459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    576..597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(612..617,618..635,636..642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    650..666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     655..677
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     674..690
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     674..690
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        711..729
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        824..1203
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1204..1407
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1358..1378
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1404..1428
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2043..2054
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    3180..3314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3217..3236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3271..3290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3332..3351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3333..3351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3358..3375)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3358..3374)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3367..3389)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3516..3971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3603..3622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3813..3834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3997..4101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4102..4170,4171..4962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4320..4339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5133..5433,1..288)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
       61 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      361 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      421 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      481 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      541 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      601 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      661 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct
      721 cactaaaggg aacaaaagct ggagcttggc ccattgcata cgttgtatcc atatcataat
      781 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
      841 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      901 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      961 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1021 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1081 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1141 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1201 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1261 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1321 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1381 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     1441 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct gactctagag
     1501 gatcccccgg gctgcagatg acggtgatgt caggggagaa tgcagacgag gcttcggccg
     1561 ctccaggtca cccccaggat ggcagctacc caaggcaggc ggaccacgac gaccacgaat
     1621 gctgcgagcg cgtggtgatc aacatctccg ggctgcgctt cgagacgcag ctcaagactc
     1681 tggcccagtt ccccaacacg ctgctgggca acccgaagaa acgcatgcgc tactttgacc
     1741 ctctgaggaa tgagtacttc tttgaccgca accggcccag cttcgatgcc atcctttatt
     1801 actaccagtc gggggggcgc ctgcgcaggc cggtcaacgt gcccctggac atgttctccg
     1861 aggagattaa attttacgag ttgggcgagg aggccatgga gaagttccgg gaagatgagg
     1921 gcttcatcaa ggaagaggag cgccccctac ccgagaagga gtaccagcgc caggtgtggc
     1981 tgctctttga gtatccggag agctcaggac ctgcacgggt tattgccatt gtatccgtca
     2041 tggtcatcct catctccata gtcatctttt gcctggagac tctccctgag ctgaaggatg
     2101 acaaggactt cacgggcacc attcaccgca tcgataacac cacagtcatc tacacttcta
     2161 acatcttcac agaccctttc ttcattgtgg aaaccttgtg tatcatctgg ttctcttttg
     2221 agctggtggt gcgcttcttc gcctgcccca gcaagacaga cttctttaag aacatcatga
     2281 acttcatcga cattgtggcc atcatccctt atttcattac cctgggcaca gagatagctg
     2341 agcaggaggg gaatcagaag ggcgagcagg ccacttccct ggccatcctc agggtcatcc
     2401 gcttggtaag ggtgttcaga atcttcaaac tctcccgcca ctccaagggc cttcagatcc
     2461 tgggccagac cctcaaagct agtatgaggg agttagggct gctcatcttt ttcctcttca
     2521 ttggcgtcat actgttttct agtgcagtgt actttgcgga ggcggaagaa gctgagtcgc
     2581 acttctccag tatccccgat gctttctggt gggcggtggt gtccatgacc actgtgggat
     2641 acggtgacat gtaccctgtg acaattggag gcaagatcgt gggctccttg tgtgccatcg
     2701 ctggtgtgct gacaattgcc ctgcccgtac ctgtcattgt gtccaatttc aactatttct
     2761 accaccgaga aactgagggg gaagagcagg ctcagttgct ccatgttagt tctcctaact
     2821 tagcctctga cagtgacctc agccgccgca gctcctctac tatcagcaag tctgagtaca
     2881 tggagatcga agaggacatg aacaatagca tagcccacta caggcaggct aatatcagaa
     2941 ctggtaactg caccgcaact gatcaaaact gcgttaataa gagcaagctc ctgaccgatg
     3001 tttaaaaaaa gcaccaggca agcaatcaaa agcccccaaa caaaaccctt ggcgactcct
     3061 gtcccactct gtagatactt tactaaaacc gtagtctttg aatgctttat ttaactggca
     3121 atgcactgtt gcattgtgaa tttggggggt gggcaaacct gaagcttatc gataccgtca
     3181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3301 atcatgtctg gatccccggg tacccaattc gccctatagt gagtcgtatt acaattcact
     3361 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3421 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3481 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     3541 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3601 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3661 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3721 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3841 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3961 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4021 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4081 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4141 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4201 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4261 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4321 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4381 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4441 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4501 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4561 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4621 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4681 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4741 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4801 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4861 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4921 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4981 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5041 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5101 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5161 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5221 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5281 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5341 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5401 gggttggact caagacgata gttaccggat aag
//
