LOCUS       sequence-388535-        5421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescence reporters for -1 ribosomal frameshifting in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Baik AH, Haribowo AG, Chen X, Queliconi BB, Barrios AM, Garg A, 
            Maishan M, Campos AR, Matthay MA, Jain IH
  TITLE     Oxygen toxicity causes cyclic damage by destabilizing specific Fe-S 
            cluster-containing protein complexes.
  JOURNAL   Mol Cell. 2023 Mar 16;83(6):942-960.e9. doi: 
            10.1016/j.molcel.2023.02.013. Epub 2023 Mar 8.
  PUBMED    36893757
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.02.013"; journalName: "Mol Cell"; date: 
            "2023-03-16- 16"; volume: "83"; issue: "6"; pages: "942-960.e9"
COMMENT     Sequence Label: pAGH10 - RlucFluc minus 1 HIV-1
FEATURES             Location/Qualifiers
     source          1..5421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      97..232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          316..448
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     431..450
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        493..511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3203..3324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3214..3233)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3268..3287
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3457..3561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3562..3630,3631..4422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3780..3799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4593..5181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5082..5101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(5310..5421,1..246)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(5310..5421,1..80)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5333..5353)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
       61 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      121 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      181 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      241 tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg
      301 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa
      361 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
      421 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa
      481 ggctagagta cttaatacga ctcactatag gctagcatga ctagcaaagt ttatgacccg
      541 gagcagcgca aacggatgat cacgggtccc cagtggtggg ctaggtgcaa gcagatgaat
      601 gtccttgaca gtttcatcaa ctattatgat tccgagaagc acgctgagaa tgcagtcata
      661 tttctgcacg gtaacgccgc gagctcctat ctgtggagac acgttgttcc tcatattgag
      721 ccagtcgctc ggtgtattat accggacctt ataggtatgg gaaagtccgg taagtcaggt
      781 aacggctcat acaggcttct tgaccactat aagtatctga ccgcctggtt tgaacttctt
      841 aaccttccca agaaaatcat ttttgtcggc catgactggg gggcgtgttt ggctttccac
      901 tattcatacg agcatcagga taaaatcaag gcaatcgtcc atgctgaaag cgtggtggac
      961 gtgatcgaat cctgggacga atggcccgat atcgaggaag atattgcgtt gattaaatca
     1021 gaagaagggg aaaaaatggt actcgagaac aacttcttcg tagaaacaat gctgccttca
     1081 aagatcatgc gcaagctgga acctgaggaa tttgctgctt acctcgagcc atttaaagag
     1141 aagggagagg taagacggcc tactctgagt tggcctaggg agattccttt ggtaaaagga
     1201 ggtaaacccg atgtcgtaca gatagtccgc aattacaacg cctatctgag agcatcagat
     1261 gatcttccca agatgtttat cgagagcgac ccagggttct tctctaatgc cattgtcgag
     1321 ggggcaaaga aattcccaaa taccgagttc gtgaaagtga aaggtctgca cttcagtcaa
     1381 gaagatgcac ctgatgagat gggaaaatat atcaaatcct ttgtggaacg agttctgaag
     1441 aatgagcaaa ccggtgatcc ttttttaggg aagatctggc cttcccacaa ggggaggcca
     1501 gggaattttc ttccactagt atggaagacg caaaaaacat taagaaaggg cccgcgccat
     1561 tttacccttt ggaggatgga accgcaggag agcaactgca caaagcaatg aaaagatacg
     1621 cccttgttcc tgggaccatt gctttcactg atgcgcacat cgaggtcaac atcacctatg
     1681 ccgaatattt cgagatgagt gtgcgcttgg ccgaagcgat gaaacgctac ggtcttaaca
     1741 caaatcatag aatagtcgtt tgtagcgaga actctttgca gttcttcatg cctgtccttg
     1801 gcgctttgtt tattggtgtg gcggttgctc cggctaacga tatttacaac gaacgggaac
     1861 tgctgaactc catgaatatc tctcagccaa ctgtggtttt cgtgagtaag aaggggcttc
     1921 agaaaatctt gaacgtacag aaaaaactgc ccattataca aaaaataatc atcatggatt
     1981 caaagactga ctatcaaggt ttccaatcta tgtatacctt tgtcactagc catctgccac
     2041 ctggatttaa tgaatacgac tttgtccccg aaagcttcga tagagataag acgatagctc
     2101 ttatcatgaa ttctagcgga agcacaggct tgccaaaggg ggtcgctctt ccacatcgga
     2161 ctgcgtgtgt ccgctttagt cacgcacggg atcccatatt tgggaaccaa atcattcctg
     2221 ataccgcaat cctcagtgta gtcccatttc atcacggatt cgggatgttc acgacgctgg
     2281 gctacctcat atgtgggttt cgcgtagtgc tcatgtacag atttgaagag gaactttttt
     2341 tgcggtctct gcaagactat aaaatacaaa gtgccctcct cgtcccaact ctttttagtt
     2401 tcttcgctaa gtccaccctg atcgataaat atgatctgag caacttgcat gagatcgctt
     2461 caggaggggc tcctctctct aaagaagtag gtgaagcggt agcaaagaga ttccatctcc
     2521 ccgggatacg acagggttat ggcctgacgg agacaacatc cgctattttg atcacgccgg
     2581 aaggcgacga taagccgggc gccgtcggca aagtcgtccc gttctttgaa gcaaaggtcg
     2641 tcgaccttga cacggggaag actctcggcg ttaaccagcg aggtgaactg tgcgtaagag
     2701 gtccaatgat aatgtctggt tatgtaaaca accccgaagc tacaaacgct ctcatagata
     2761 aagacggttg gctccacagc ggagacattg catactggga cgaagatgag cattttttca
     2821 ttgttgatcg acttaaatcc cttattaaat acaaaggata ccaggttgcg ccagcagaac
     2881 tcgagtctat ccttcttcag catccgaata tatttgatgc aggcgtggca ggtctgccgg
     2941 acgacgatgc tggcgaactt cctgcggcgg tagttgtcct ggaacacggc aagaccatga
     3001 cagaaaaaga aatagtagac tatgtcgcca gtcaggtcac tacagccaaa aagctgcgag
     3061 gtggcgttgt atttgtagac gaagttccga agggcttgac cgggaaactc gacgctagga
     3121 aaatccgaga aatccttatc aaagcaaaaa aaggtggaaa gtccaagctt taatctagag
     3181 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3361 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3421 gataaggatc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3781 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4081 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4561 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5161 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5221 tgctggcctt ttgctcacat ggctcgacag atctgcgcag caccatggcc tgaaataacc
     5281 tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg
     5341 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5401 catctcaatt agtcagcaac c
//
