LOCUS       sequence-38854-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene was not detected in the brain and 
            eye using LacZ. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1518
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          232..364
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
       61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacgggatc cgccatggtg
      181 gcggaagttc ctataccttt tgaagaatag gaacttcgga ataggaactt cctgtggaga
      241 gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc aagagtcttc
      301 tctgtctcga caagcccagt ttctattggt ctccttaaac ctgtcttgta accttgatac
      361 ttacggcgcg ccggaacctc tggatctgcc agtcagccac cctgtcctgc tcagacttta
      421 ccagagacga gtatttccct ttccttccct ctaagagcat tttatttgca gagttagcca
      481 atagtatctt gtttcctgta agggagacta tctttagatg ctgccacagg caagctcaga
      541 tactccaggg tggaggccag ctgccaacag gagggcctga ctttataaac ctggaagatc
      601 taaacctcag ggaagactgc ggagctggag ggagagaggt gccacttacc ccaacctgga
      661 ggtggggggc agggagtagg ccctgcagtc agcaaagcac agcacagtca gctctcatta
      721 atggggagcc ttcaggaagc tgggagagct catggggccc tcagcttccc tgcccatgag
      781 agggagccca
//
