LOCUS       sequence-388564-        6761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a human PTPN22 mutant (620W) with 
            sfCherry(11) tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6761)
  TITLE     Concannon Lab Plasmids
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfCherry11-620W-PTPN22
FEATURES             Location/Qualifiers
     source          1..6761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..49
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        304..607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        608..811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     762..782
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        857..875
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3499..3518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3500..3518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3792..3913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3829..3848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3883..3902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3920..4375)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4057..4078)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4269..4288
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4402..4506
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4508..4865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4508..4699
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4531..4551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4716..4851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4900..5694
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4900..5694
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4954..4973)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5564..5583
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5882..5901)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5926..5973
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(6302..6761,1..129)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
       61 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      121 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      181 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
      241 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      361 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      421 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      481 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      541 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      601 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      661 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      721 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      781 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
      841 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg
      901 gtggcggccg ccaccatgta caccatcgtg gagcagtacg agagagccga ggccagacac
      961 agcaccggtg gcggcatgga ccaaagagaa attctgcaga agttcctgga tgaggcccaa
     1021 agcaagaaaa ttactaaaga ggagtttgcc aatgaatttc tgaagctgaa aaggcaatct
     1081 accaagtaca aggcagacaa aacctatcct acaactgtgg ctgagaagcc caagaatatc
     1141 aagaaaaaca gatataagga tattttgccc tatgattata gccgggtaga actatccctg
     1201 ataacctctg atgaggattc cagctacatc aatgccaact tcattaaggg agtttatgga
     1261 cccaaggctt atattgccac ccagggtcct ttatctacaa ccctcctgga cttctggagg
     1321 atgatttggg aatatagtgt ccttatcatt gttatggcat gcatggagta tgaaatggga
     1381 aagaaaaagt gtgagcgcta ctgggctgag ccaggagaga tgcagctgga atttggccct
     1441 ttctctgtat cctgtgaagc tgaaaaaagg aaatctgatt atataatcag gactctaaaa
     1501 gttaagttca atagtgaaac tcgaactatc taccagtttc attacaagaa ttggccagac
     1561 catgatgtac cttcatctat agaccctatt cttgagctca tctgggatgt acgttgttac
     1621 caagaggatg acagtgttcc catatgcatt cactgcagtg ctggctgtgg aaggactggt
     1681 gttatttgtg ctattgatta tacatggatg ttgctaaaag atgggataat tcctgagaac
     1741 ttcagtgttt tcagtttgat ccgggaaatg cggacacaga ggccttcatt agttcaaacg
     1801 caggaacaat atgaactggt ctacaatgct gtattagaac tatttaagag acagatggat
     1861 gttatcagag ataaacattc tggaacagag agtcaagcaa agcattgtat tcctgagaaa
     1921 aatcacactc tccaagcaga ctcttattct cctaatttac caaaaagtac cacaaaagca
     1981 gcaaaaatga tgaaccaaca aaggacaaaa atggaaatca aagaatcttc ttcctttgac
     2041 tttaggactt ctgaaataag tgcaaaagaa gagctagttt tgcaccctgc taaatcaagc
     2101 acttcttttg actttctgga gctaaattac agttttgaca aaaatgctga cacaaccatg
     2161 aaatggcaga caaaggcatt tccaatagtt ggggagcctc ttcagaagca tcaaagtttg
     2221 gatttgggct ctcttttgtt tgagggatgt tctaattcta aacctgtaaa tgcagcagga
     2281 agatatttta attcaaaggt gccaataaca cggaccaaat caactccttt tgaattgata
     2341 cagcagagag aaaccaagga ggtggacagc aaggaaaact tttcttattt ggaatctcaa
     2401 ccacatgatt cttgttttgt agagatgcag gctcaaaaag taatgcatgt ttcttcagca
     2461 gaactgaatt attcactgcc atatgactct aaacaccaaa tacgtaatgc ctctaatgta
     2521 aagcaccatg actctagtgc tcttggtgta tattcttaca tacctttagt ggaaaatcct
     2581 tatttttcat catggcctcc aagtggtacc agttctaaga tgtctcttga tttacctgag
     2641 aagcaagatg gaactgtttt tccttcttct ctgttgccaa catcctctac atccctcttc
     2701 tcttattaca attcacatga ttctttatca ctgaattctc caaccaatat ttcctcacta
     2761 ttgaaccagg agtcagctgt actagcaact gctccaagga tagatgatga aatcccccct
     2821 ccacttcctg tatggacacc tgaatcattt attgtggttg aggaagctgg agaattctca
     2881 ccaaatgttc ccaaatcctt atcctcagct gtgaaggtaa aaattggaac atcactggaa
     2941 tggggtggaa catctgaacc aaagaaattt gatgactctg tgatacttag accaagcaag
     3001 agtgtaaaac tccgaagtcc taaatcagaa ctacatcaag atcgttcttc tcccccacct
     3061 cctctcccag aaagaactct agagtccttc tttcttgccg atgaagattg tatgcaggcc
     3121 caatctatag aaacatattc tactagctat cctgacacca tggaaaattc aacatcttca
     3181 aaacagacac tgaagactcc tggaaaaagt ttcacaagga gtaagagttt gaaaattttg
     3241 cgaaacatga aaaagagtat ctgtaattct tgcccaccaa acaagcctgc agaatctgtt
     3301 cagtcaaata actccagctc atttctgaat tttggttttg caaaccgttt ttcaaaaccc
     3361 aaaggaccaa ggaatccacc accaacttgg aatatttaat aaaactccag atttataata
     3421 atatgggctg caagtacacc tcgagggggg gcccggtacc ttaattaatt aaggtaccag
     3481 gtaagtgtac ccaattcgcc ctatagtgag tcgtattaca attcactcga tcgcccttcc
     3541 caacagttgc gcagcctgaa tggcgaatgg agatccaatt tttaagtgta taatgtgtta
     3601 aactactgat tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc
     3661 ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt
     3721 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3781 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3841 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3901 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     3961 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4021 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4081 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4141 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4201 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4261 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4321 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     4381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4501 aagaatcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4561 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4621 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4681 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4741 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4801 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4861 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4921 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4981 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5041 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     5101 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5161 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5221 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5281 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5341 atggaagccg gtcttgtcga tcaggatgat ctggacgaag aacatcaggg gctcgcgcca
     5401 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     5461 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5521 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5581 attgctgaag aacttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5641 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5701 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5761 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5821 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     5881 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     5941 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     6001 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     6061 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     6121 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     6181 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6421 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg a
//
