LOCUS       sequence-38860-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene was not detected in the brain and 
            eye using LacZ. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1529
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     143..165
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          235..367
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
       61 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
      121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggg atccgccatg
      181 gtggcggaag ttcctatacc ttttgaagaa taggaacttc ggaataggaa cttcctgtgg
      241 agagaaaggc aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc
      301 ttctctgtct cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga
      361 tacttacggc gcgccggtgt ctgggctctg tcagaggtga aaagcagccg ggagtgactt
      421 gctctctgtc ctctctctct cctctccaca gtgacagtta agaggcacag aagatgtgat
      481 tgccatttat gtagacagag tgtctgcctc cccgaagcca atcagagctg ccggctctgc
      541 attatttact gagcagcatc cctccaccct cccaccacct cctgccccca ctctgccccc
      601 agggggagag agccatccta gcaggatgga gaggagcatc agggcctgct gggactggcg
      661 agaggccaag gaaaaaatgg gccctgtgtt cccaggcact gtgtaggatg cagatttggg
      721 gccagggctc ctgggactct cccagcaact ttga
//
