LOCUS       sequence-388605-        8813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas12a gRNA expression backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pKL038 Lenti U6 dCas12a gRNA Puro mCherry
FEATURES             Location/Qualifiers
     source          1..8813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(273..284)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(393..409)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(394..410)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             919..1099
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(1161..1749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1241..1260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1920..2711,2712..2780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2543..2562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2781..2885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2960..2979)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3151..3530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3532..3730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3685..3705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3748..3928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3975..4100
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4597..4830
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5015..5059
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5357..5474
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5547..5795
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5547..5567
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5718..5737
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        5967..6178
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          6179..7117
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7074..7094
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7133..7729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7133..7152)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7629..7649
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7730..7783
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      7790..7799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7796..8506
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7941..7959)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8209..8228
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(8526..8559)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(8615..8813,1..390)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8668..8688)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
       61 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
      121 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
      181 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
      241 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
      301 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
      361 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
      421 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
      481 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
      541 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
      601 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
      661 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
      721 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
      781 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
      841 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
      901 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     1021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1081 gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1141 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1201 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1261 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1321 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1381 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1501 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1561 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1621 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1681 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1741 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1801 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1861 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1921 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1981 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2041 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2101 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2161 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2221 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2281 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2341 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2401 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2461 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2521 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2581 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2641 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2701 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2761 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2821 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2881 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     2941 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     3001 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     3061 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     3121 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     3181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     3421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3541 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3661 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3721 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     3781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3901 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     3961 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     4021 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     4081 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     4141 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     4201 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     4261 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     4321 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     4381 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     4441 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     4501 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     4561 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     4621 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     4681 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     4741 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     4801 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     4861 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     4921 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     4981 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     5041 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     5101 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     5161 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     5221 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     5281 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     5341 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5401 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5461 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttagtaccgg
     5521 gcccgctcta gaaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc
     5581 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     5641 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     5701 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     5761 tggctttata tatcttgtgg aaaggacgaa acaccgtaat ttctactctt gtagatggag
     5821 acgattaatg cgtctccaat ttctactctt gtagattttt tttaagcttg ggccgctcga
     5881 ggtacctctc tacatatgac atgtgagcgg atccattagg cggccgcgtg gataaccgta
     5941 ttaccgccat gcatgtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     6001 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     6061 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     6121 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     6181 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     6241 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     6301 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     6361 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     6421 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     6481 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     6541 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     6601 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     6661 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     6721 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     6781 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     6841 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     6901 cctcgattag ttctcgcgct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     6961 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     7021 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     7081 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcta gcatgaccga
     7141 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     7201 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     7261 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7321 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7381 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7441 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7501 gttcctggcc accgtcggcg tatcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7561 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7621 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7681 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg agggcagagg
     7741 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaccggtcg ccaccatggt
     7801 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     7861 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     7921 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     7981 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     8041 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     8101 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     8161 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     8221 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     8281 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     8341 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     8401 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     8461 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagaat tcgtcgaggg
     8521 acctaataac ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt
     8581 ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     8641 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8701 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8761 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aac
//
