LOCUS       sequence-388608-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5_S1661X in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc_S1661X
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        114..471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        114..305
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(137..157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      322..457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             502..1539
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             1518..1526
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    1563..1611
                     /note="synthetic polyadenylation signal"
     promoter        1767..1871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1872..1940,1941..2732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2090..2109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(2890..3478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2970..2989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3594..3877
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     enhancer        4068..4447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4448..4651
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4602..4622
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          4787..4919
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4902..4921
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     4963..4982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4963..4981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10085..10597
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(10673..10691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    10728..10849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10739..10758)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10793..10812
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
       61 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
      121 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
      181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgat
      481 tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag cgttgaaaaa
      541 tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga gggcgaagag
      601 agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt gaatagctgc
      661 gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc tgcactaccc
      721 atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg catcagtaga
      781 cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc tgtgttacag
      841 cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac cagcggcttc
      901 ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt catttgcgcc
      961 attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt gtccgccagc
     1021 gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga ggtgcgccac
     1081 ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg catcaccgcc
     1141 gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc caacatcttc
     1201 ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga gcgccggcat
     1261 cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg cctggatcag
     1321 ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca aggccgctgc
     1381 gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc tcgccggagc
     1441 gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa ccgccggccc
     1501 agtacacgac cgcgcgctaa ggaggtaggt cgagtttaga ctctagccat cacgatggcc
     1561 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     1621 cgataaggat cctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     1681 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtaatggtt tcttagacgt
     1741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1861 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1921 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1981 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2041 gttttcgccc cgaagaacgt tttccaatga tgagcacttt caaagttctg ctatgtggcg
     2101 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2161 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2221 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2281 tgacaactat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2341 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2401 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2461 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2521 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2581 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2641 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2701 agataggtgc ctcactgatt aagcattggt aattcgaaat gaccgaccaa gcgacgccca
     2761 accggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2821 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2881 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2941 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3001 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3061 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3121 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3181 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3241 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3301 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3361 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3421 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga
     3481 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3541 gattttggtc atgagattat caaaaaggat cttcacctag atccttttat agtccggaaa
     3601 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
     3661 ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac
     3721 ggttaaaatt tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac
     3781 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat
     3841 ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca
     3901 cgatgcgtcc ggcgtagagg atcgagatct tcaatattgg ccattagcca tattattcat
     3961 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
     4021 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt
     4081 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     4141 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     4201 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     4261 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     4321 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     4381 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     4441 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     4501 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     4561 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
     4621 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tcactagaag
     4681 ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac
     4741 agtctcgaac ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt
     4801 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     4861 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     4921 tgtccactcc cagttcaatt acagctctta aggctagagt attaatacga ctcactatag
     4981 ggctagcaaa gcgatcgcca tgaaactgcg tggagtcagc ctggctgccg gcttgttctt
     5041 actggccctg agtctttggg ggcagcctgc agaggctgcg gcttgctatg ggtgttctcc
     5101 aggatcaaag tgtgactgca gtggcataaa aggggaaaag ggagagagag ggtttccagg
     5161 tttggaagga cacccaggat tgcctggatt tccaggtcca gaagggcctc cggggcctcg
     5221 gggacaaaag ggtgatgatg gaattccagg gccaccagga ccaaaaggaa tcagaggtcc
     5281 tcctggactt cctggatttc cagggacacc aggtcttcct ggaatgccag gccacgatgg
     5341 ggccccagga cctcaaggta ttcccggatg caatggaacc aagggagaac gtggatttcc
     5401 aggcagtccc ggttttcctg gtttacaggg tcctccagga ccccctggga tcccaggtat
     5461 gaagggtgaa ccaggtagta taattatgtc atcactgcca ggaccaaagg gtaatccagg
     5521 atatccaggt cctcctggaa tacaaggcct acctggtccc actggtatac cagggccaat
     5581 tggtccccca ggaccaccag gtttgatggg ccctcctggt ccaccaggac ttccaggacc
     5641 taaggggaat atgggcttaa atttccaggg acccaaaggt gaaaaaggtg agcaaggtct
     5701 tcagggccca cctgggccac ctgggcagat cagtgaacag aaaagaccaa ttgatgtaga
     5761 gtttcagaaa ggagatcagg gacttcctgg tgaccgaggg cctcctggac ctccagggat
     5821 acgtggtcct ccaggtcccc caggtggtga gaaaggtgag aagggtgagc aaggagagcc
     5881 aggcaaaaga ggtaaaccag gcaaagatgg agaaaatggc caaccaggaa ttcctggttt
     5941 gcctggtgat cctggttacc ctggtgaacc cggaagggat ggtgaaaagg gccaaaaagg
     6001 tgacactggc ccacctggac ctcctggact tgtaattcct agacctggga ctggtataac
     6061 tataggagaa aaaggaaaca ttgggttgcc tgggttgcct ggagaaaaag gagagcgagg
     6121 atttcctgga atacagggtc cacctggcct tcctggacct ccaggggctg cagttatggg
     6181 tcctcctggc cctcctggat ttcctggaga aaggggtcag aaaggtgatg aaggaccacc
     6241 tggaatttcc attcctggac ctcctggact tgacggacag cctggggctc ctgggcttcc
     6301 agggcctcct ggccctgctg gccctcacat tcctcctagt gatgagatat gtgaaccagg
     6361 ccctccaggc cccccaggat ctccaggtga taaaggactc caaggagaac aaggagtgaa
     6421 aggtgacaaa ggtgacactt gcttcaactg cattggaact ggtatttcag ggcctccagg
     6481 tcaacctggt ttgccaggtc tcccaggtcc tccaggatct cttggtttcc ctggacagaa
     6541 aggggaaaaa ggacaagctg gtgcaactgg tcccaaagga ttaccaggca ttccaggagc
     6601 tccaggtgct ccaggctttc ctggatctaa aggtgaacct ggtgatatcc tcacttttcc
     6661 aggaatgaag ggtgacaaag gagagttggg ttcccctgga gctccagggc ttcctggttt
     6721 acctggcact cctggacagg atggattgcc agggcttcct ggcccgaaag gagagcctgg
     6781 tggaattact tttaagggtg aaagaggtcc ccctgggaac ccaggtttac caggcctccc
     6841 agggaatata gggcctatgg gtccccctgg tttcggccct ccaggcccag taggtgaaaa
     6901 aggcatacaa ggtgtggcag gaaatccagg ccagccagga ataccaggtc ctaaagggga
     6961 tccaggtcag actataaccc agccggggaa gcctggcttg cctggtaacc caggcagaga
     7021 tggtgatgta ggtcttccag gtgaccctgg acttccaggg caaccaggct tgccagggat
     7081 acctggtagc aaaggagaac caggtatccc tggaattggg cttcctggac cacctggtcc
     7141 caaaggcttt cctggaattc caggacctcc aggagcacct gggacacctg gaagaattgg
     7201 tctagaaggc cctcctgggc cacccggctt tccaggacca aagggtgaac caggatttgc
     7261 attacctggg ccacctgggc caccaggact tccaggtttc aaaggagcac ttggtccaaa
     7321 aggtgatcgt ggtttcccag gacctccggg tcctccagga cgcactggct tagatgggct
     7381 ccctggacca aaaggtgatg ttggaccaaa tggacaacct ggaccaatgg gacctcctgg
     7441 gctgccagga ataggtgttc agggaccacc aggaccacca gggattcctg ggccaatagg
     7501 tcaacctggt ttacatggaa taccaggaga gaagggggat ccaggacctc ctggacttga
     7561 tgttccagga cccccaggtg aaagaggcag tccagggatc cccggagcac ctggtcctat
     7621 aggacctcca ggatcaccag ggcttccagg aaaagcaggt gcctctggat ttccaggtac
     7681 caaaggtgaa atgggtatga tgggacctcc aggcccacca ggacctttgg gaattcctgg
     7741 caggagtggt gtacctggtc ttaaaggtga tgatggcttg cagggtcagc caggacttcc
     7801 tggccctaca ggagaaaaag gtagtaaagg agagcctggc cttccaggcc ctcctggacc
     7861 aatggatcca aatcttctgg gctcaaaagg agagaagggg gaacctggct taccaggtat
     7921 acctggagtt tcagggccaa aaggttatca gggtttgcct ggagacccag ggcaacctgg
     7981 actgagtgga caacctggat taccaggacc accaggtccc aaaggtaacc ctggtctccc
     8041 tggacagcca ggtcttatag gacctcctgg acttaaagga accatcggtg atatgggttt
     8101 tccagggcct cagggtgtgg aagggcctcc tggaccttct ggagttcctg gacaacctgg
     8161 ctccccagga ttacctggac agaaaggcga caaaggtgat cctggtattt caagcattgg
     8221 tcttccaggt cttcctggtc caaagggtga gcctggtctg cctggatacc cagggaaccc
     8281 tggtatcaaa ggttctgtgg gagatcctgg tttgcccgga ttaccaggaa cccctggagc
     8341 aaaaggacaa ccaggccttc ctggattccc aggaacccca ggccctcctg gaccaaaagg
     8401 tattagtggc cctcctggga accccggcct tccaggagaa cctggtcctg taggtggtgg
     8461 aggtcatcct gggcaaccag ggcctccagg cgaaaaaggc aaacccggtc aagatggtat
     8521 tcctggacca gctggacaga agggtgaacc aggtcaacca ggctttggaa acccaggacc
     8581 gcctggactt ccaggacttt ctggccaaaa gggtgatgga ggattacctg ggattccagg
     8641 aaatcctggc cttccaggtc caaagggcga accaggcttt cacggtttcc ctggtgtgca
     8701 gggtccccca ggccctcctg gttctccggg tccagctctg gaaggaccta aaggcaaccc
     8761 tgggccccaa ggtcctcctg ggagaccagg tcctacaggt tttcaaggtc taccaggtcc
     8821 agaaggtcct ccaggtctcc ctggaaatgg aggtattaaa ggagagaagg gaaatccagg
     8881 ccaacctggg ctacctggct tgcctggttt gaaaggagat caaggaccac caggactcca
     8941 gggtaatcct ggccggccgg gtctcaatgg aatgaaagga gatcctggtc tccctggtgt
     9001 tccaggattc ccaggcatga aaggacccag tggagtacct ggatcagctg gccctgaggg
     9061 ggaaccggga cttattggtc ctccaggtcc tcctggatta cctggtcctt caggacagag
     9121 tatcataatt aaaggagatg ctggtcctcc aggaatccct ggccagcctg ggctaaaggg
     9181 tctaccagga ccccaaggac ctcaaggctt accaggtcca actggccctc caggagatcc
     9241 tggacgcaat ggactccctg gctttgatgg tgcaggaggg cgcaaaggag acccaggtct
     9301 gccaggacag ccaggtaccc gtggtttgga tggtccccct ggtccagatg gattgcaagg
     9361 tcccccaggt ccccctggaa cctcctctgt tgcacatgga tttcttatta cacgccacag
     9421 ccagacaacg gatgcaccac aatgcccaca gggaacactt caggtctatg aaggcttttc
     9481 tctcctgtat gtacaaggaa ataaaagagc ccacggtcaa gacttgggga cggctggcag
     9541 ctgccttcgt cgctttagta ccatgccttt catgttctgc aacatcaata atgtttgcaa
     9601 ctttgcttca agaaatgact attcttactg gctctctacc ccagagccca tgccaatgag
     9661 catgcaaccc ctaaagggcc agagcatcca gccattcatt agtcgatgtg cagtatgtga
     9721 agctccagct gtggtgatcg cagttcacag tcagacgatc cagattcccc attgtcctca
     9781 gggatgggat tctctgtgga ttggttattc cttcatgatg catacaagtg caggggcaga
     9841 aggctcaggt caagccctag cctcccctgg ttcctgcttg gaagagtttc gttcagctcc
     9901 cttcatcgaa tgtcatggga ggggtacctg taactactat gccaactcct acagcttttg
     9961 gctggcaact gtagatgtgt aagacatgtt cagtaaacct cagtcagaaa cgctgaaagc
    10021 aggagacttg aggacacgaa ttagccgatg tcaagtgtgc atgaagagga cattctcgag
    10081 cggcgtcttc acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct
    10141 ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt
    10201 aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt
    10261 catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa
    10321 ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt
    10381 aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc
    10441 cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat
    10501 cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt
    10561 gaccggctgg cggctgtgcg aacgcattct ggcgtaaggc cgcgactcta gggcatgcaa
    10621 gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
    10681 caataactag cataacccct tggggcggcc gcttcgagca gacatgataa gatacattga
    10741 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
    10801 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
    10861 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
    10921 aaacctctac aaatgtggta aaatcgaatt ttaacaaaat attaacgctt acaatttcct
//
