LOCUS       sequence-388617-        5826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Vaaben TH, Vazquez-Uribe R, Sommer MOA
  TITLE     Characterization of Eight Bacterial Biosensors for Microbial 
            Diagnostic and Therapeutic Applications.
  JOURNAL   ACS Synth Biol. 2022 Dec 16;11(12):4184-4192. doi: 
            10.1021/acssynbio.2c00491. Epub 2022 Nov 30.
  PUBMED    36449712
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00491"; journalName: "ACS Synth Biol"; date: 
            "2022-12-16- 16"; volume: "11"; issue: "12"; pages: "4184-4192"
COMMENT     Sequence Label: pMUT2-pAto-mCherry
FEATURES             Location/Qualifiers
     source          1..5826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..98
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     321..338
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(960..1754)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1755..1859)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2160..2182
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2174..2191
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2175..2191
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2202..2221
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2202..2220
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2225..2236
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
       61 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      121 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      181 cgcctcccgc gcgcccttct tctgggtctt tgtcttttgc tcacatgttc tttccggttt
      241 tatcccccga ttctgtggat aaccgtatta ccgcctttga gtgagctgac accgctcgcc
      301 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgccttatgt
      361 gacattttct ccttacgctc ctctatgccg ttctgcatcc tgtccggatg cgttatatcc
      421 cggtaagatt ttccgcttca aagcgtgtct gtatgctgtt ctggagttct tctgcgagtt
      481 cgtgcagttt ctcacacatg gcggcctgtt cgtcggcatt gagtgcgtcc agtttttcga
      541 gcagcgtcag gctctgactt tttatgaatc ccgccatgtt gagtacggct tgctgctgct
      601 tattcatctt ttcgttttct ccgttctgtc tgtcatctgc gttgtgtgat tatatcgcgt
      661 accacttttc gactgttttg ctgccgctat tctgccgctt ggctttttga cgggcatttc
      721 tgtcagacaa cactgtcact gccaaaaaac tgccgtgcct ttgtcggtaa ttcgagcttg
      781 ctgacaggac aggatgtgca attgttatac cgcgcataca tgcacgctat tacaattgcc
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
      961 tagaaaaatt catccagcag acgataaaac gcaatacgct ggctatccgg tgccgcaatg
     1021 ccatacagca ccagaaaacg atccgcccat tcgccgccca gttcttccgc aatatcacgg
     1081 gtggccagcg caatatcctg ataacgatcc gccacgccca gacggccgca atcaataaag
     1141 ccgctaaaac ggccattttc caccataatg ttcggcaggc acgcatcacc atgggtcacc
     1201 accagatctt cgccatccgg catgctcgct ttcagacgcg caaacagctc tgccggtgcc
     1261 aggccctgat gttcttcatc cagatcatcc tgatccacca ggcccgcttc catacgggta
     1321 cgcgcacgtt caatacgatg tttcgcctga tgatcaaacg gacaggtcgc cgggtccagg
     1381 gtatgcagac gacgcatggc atccgccata atgctcactt tttctgccgg cgccagatgg
     1441 ctagacagca gatcctgacc cggcacttcg cccagcagca gccaatcacg gcccgcttcg
     1501 gtcaccacat ccagcaccgc cgcacacgga acaccggtgg tggccagcca gctcagacgc
     1561 gccgcttcat cctgcagctc gttcagcgca ccgctcagat cggttttcac aaacagcacc
     1621 ggacgaccct gcgcgctcag acgaaacacc gccgcatcag agcagccaat ggtctgctgc
     1681 gcccaatcat agccaaacag acgttccacc cacgctgccg ggctacccgc atgcaggcca
     1741 tcctgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1861 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     1921 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     1981 ataaatcaaa agaatagacc gagatagggt tgagtggccg ctacagggcg ctcccattcg
     2041 ccattcaggc tgcgcaactg ttgggaaggg cgtttcggtg cgggcctctt cgctattacg
     2101 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2161 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgac gtaatacgac tcactatagg
     2221 gcgaattgaa ggaaggccgt caaggccacg tgtcttgtcc gcggtaccta cgccgtgcat
     2281 tgatgtataa actccaggaa tacggtatcg atccggcgga tgtataacgc caaaacttgc
     2341 tatgcagaaa tttgcacagt gcgcaatttt ctgcatagcc gctcattctc cttatgaatc
     2401 cccatctaat ttatcctttt ctattcaatt agttaaataa caacattcaa taatctcatt
     2461 ctggcactcc ccttgctatt ccctgactgt acccacaacg gtgtattcag gagaattaca
     2521 atggtttcca agggcgagga ggataacatg gctatcatta aagagttcat gcgcttcaaa
     2581 gttcacatgg agggttctgt taacggtcac gagttcgaga tcgaaggcga aggcgagggc
     2641 cgtccgtatg aaggcaccca gaccgccaaa ctgaaagtga ctaaaggcgg cccgctgcct
     2701 tttgcgtggg acatcctgag cccgcaattt atgtacggtt ctaaagcgta tgttaaacac
     2761 ccagcggata tcccggacta tctgaagctg tcttttccgg aaggtttcaa gtgggaacgc
     2821 gtaatgaatt ttgaagatgg tggtgtcgtg accgtcactc aggactcctc cctgcaggat
     2881 ggcgagttca tctataaagt taaactgcgt ggtactaatt ttccatctga tggcccggtg
     2941 atgcagaaaa agacgatggg ttgggaggcg tctagcgaac gcatgtatcc ggaagatggt
     3001 gcgctgaaag gcgaaattaa acagcgcctg aaactgaaag atggcggcca ttatgacgct
     3061 gaagtgaaaa ccacgtacaa agccaagaaa cctgtgcagc tgcctggcgc gtacaatgtg
     3121 aatattaaac tggacatcac ctctcataat gaagattata cgatcgtaga gcaatatgag
     3181 cgcgcggagg gtcgtcattc taccggtggc atggatgagc tgtacaaata aaaaggggct
     3241 accggcgaac cagcagcccc tttataaagg cgcttcagta gtcagaccag catcagtcct
     3301 gaaaaggcgg gcctgcgccc gcctccaggt tgctacttac cggattcgta agccatgaaa
     3361 gccgccacct ccctgtgtcc gtctctgtaa cgaatctcgc acagcgattt tcgtgtcaga
     3421 taagtgaata tcaacagtgt gagacacacg atcaacacac accagacaag ggaacttcgt
     3481 ggtagtttca tggccttctt ctccttgcgc aaagcgcggt aagaggctat cctgatgtgg
     3541 actagacata gggatgcctc gtggtggtta atgaaaatta acttactacg gggctatctt
     3601 ctttctgcca cacaacacgg caacaaacca ccttcacgtc atgaggcaga aagcctcaag
     3661 cgccgggcac atcatagccc atatacctgc acgctgacca cactcacttt ccctgaaaat
     3721 aatccgctca ttcagaccgt tcacgggaaa tccgtgtgat tgttgccgca tcacgctgcc
     3781 tcccggagtt tgttcggcat aagtcacaag agacactcaa tggcaaagga agaccgtgcc
     3841 ctgttatcca ggcttatggc agattagctt cccggagaga aaactgtcga aaactgacgg
     3901 tatgaacacc gtaagctccc aaagtgatca ccattcgctt tcatgcatag ctatgcagcg
     3961 agctgaaaac gatcctgacg catccttcct gtttttccgg ggtaaaacat ctctttttgc
     4021 ggtgtctgcg tcagaatcgc gttcagcgcg tttcagtggt gcgtacaatt aagggattat
     4081 ggtaaatata tgagctatgc gataacttta actgtgaagc gatgaaccca ttacaggcaa
     4141 agccaattac tcctgacagt ggtttagcca gaagcagggc taccaagacc aatgcaataa
     4201 gtaatatatc gttttgctat cgtgcatcgc tcgcgctcag ttccattgtg cttttttaag
     4261 ctgtcgtttt tcttacggta tataccggtt ttttatggcg tggtttctta acttgttcag
     4321 ctactgatgg acccattgta tctaggtagt caactagctt tgttagatca taaatattgc
     4381 gaccaccata tcggcgatca ctcttcgatg ttggtttctt gtccaagaga ttagcttttt
     4441 caagatcatt gatagctctc tgaacagtcc gtacagaaac ccccatacgt atggctagac
     4501 tttccattga cggatgcggc cactcttgca aactccacca gtgaacgatc aggttaagta
     4561 gtgtgttaaa ggccactgaa gttagctttt tctcgttttg tataaaaaac aatacggtag
     4621 gcactgctgt ccagccaaga gacaaaccgc cagctttcca tttattctta acggagtaag
     4681 tcattgattt tcctaagccc caaaatattt aaagtatata ttatatggta tattcatatg
     4741 aatagggtga cactggcgcc attattgtgc aaccaaaaaa gactactctg aaaacgagga
     4801 aaagattttt tcctgcctga attagatacg gagttagcga tatgaaaacc gaacaacgtc
     4861 atgatcttgt taaagatatt gaggtttttg gcgtatcctt gtctctgttg atttccagag
     4921 cgaatgagaa gtctgttaca atgccatctg gtctaagtcg ggagcagaga agagcatggg
     4981 cagcggagca ggcgcgcaaa atccacaatt gaatattgtc tcattctctg agaccttcaa
     5041 cctttattac acatccagat attctgcaaa aacactcgat aaaatcgatg atttcattga
     5101 gcattttgaa aaatacaatc tctttggcga tcctttaaaa ggatatccag cttggactgg
     5161 caaagtatcg ccatcgtgga aagtgcctga tcattacgaa aacaaagaag ctattgagaa
     5221 gtatgctaga gctaacaaat tatggcatgc tcatttaggc gatccggttt ttaaagatac
     5281 gtttcatggg aaatacaagg tttctgactg ggttattcat ttccagcggc tgacaccgaa
     5341 ccatataaag cttttagagc ttggatacca tgacccaatg aagctacctc aaaactttga
     5401 atgatcgagc ggcaggctaa atgaaatctt gagattcatt cagtctcgtc gtaatctcac
     5461 tattgtaaaa acgaaaaaac caccctggca ggtggttttt cgaaggttag ttaatcctgg
     5521 cagattctct aaccgtggta acagtcttgt gcgagacatg tcaccaaata ctgtcctttc
     5581 agtgtagcct cagttaggcc gccacttcaa gaactctcgt tacatctctc gcacatcctg
     5641 cttaccagtg gccgttgcca gtggcgttaa gtcgtgtctt accgggttgg actcaagacg
     5701 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5761 cttggagcga acgacctaca ccgaaccgag ataccaacag cgtgagctat gagaaagcgc
     5821 cacgct
//
