LOCUS       sequence-388622-        5741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMUT2 based vector for sensing pH in E. coli Nissle 1917.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Vaaben TH, Vazquez-Uribe R, Sommer MOA
  TITLE     Characterization of Eight Bacterial Biosensors for Microbial 
            Diagnostic and Therapeutic Applications.
  JOURNAL   ACS Synth Biol. 2022 Dec 16;11(12):4184-4192. doi: 
            10.1021/acssynbio.2c00491. Epub 2022 Nov 30.
  PUBMED    36449712
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00491"; journalName: "ACS Synth Biol"; date: 
            "2022-12-16- 16"; volume: "11"; issue: "12"; pages: "4184-4192"
COMMENT     Sequence Label: pMUT2-pCadBA-mCherry
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..89
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(711..1505)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1506..1610)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1911..1933
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1925..1942
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1926..1942
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1953..1972
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1953..1971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1976..1987
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5571..5590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attctgtgga taaccgtatt accgcctttg agtgagctga caccgctcgc cgcagtcgaa
       61 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgccttatg tgacattttc
      121 tccttacgct cctctatgcc gttctgcatc ctgtccggat gcgttatatc ccggtaagat
      181 tttccgcttc aaagcgtgtc tgtatgctgt tctggagttc ttctgcgagt tcgtgcagtt
      241 tctcacacat ggcggcctgt tcgtcggcat tgagtgcgtc cagtttttcg agcagcgtca
      301 ggctctgact ttttatgaat cccgccatgt tgagtacggc ttgctgctgc ttattcatct
      361 tttcgttttc tccgttctgt ctgtcatctg cgttgtgtga ttatatcgcg taccactttt
      421 cgactgtttt gctgccgcta ttctgccgct tggctttttg acgggcattt ctgtcagaca
      481 acactgtcac tgccaaaaaa ctgccgtgcc tttgtcggta attcgagctt gctgacagga
      541 caggatgtgc aattgttata ccgcgcatac atgcacgcta ttacaattgc ccgttaaggg
      601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ttagaaaaat
      721 tcatccagca gacgataaaa cgcaatacgc tggctatccg gtgccgcaat gccatacagc
      781 accagaaaac gatccgccca ttcgccgccc agttcttccg caatatcacg ggtggccagc
      841 gcaatatcct gataacgatc cgccacgccc agacggccgc aatcaataaa gccgctaaaa
      901 cggccatttt ccaccataat gttcggcagg cacgcatcac catgggtcac caccagatct
      961 tcgccatccg gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc caggccctga
     1021 tgttcttcat ccagatcatc ctgatccacc aggcccgctt ccatacgggt acgcgcacgt
     1081 tcaatacgat gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag ggtatgcaga
     1141 cgacgcatgg catccgccat aatgctcact ttttctgccg gcgccagatg gctagacagc
     1201 agatcctgac ccggcacttc gcccagcagc agccaatcac ggcccgcttc ggtcaccaca
     1261 tccagcaccg ccgcacacgg aacaccggtg gtggccagcc agctcagacg cgccgcttca
     1321 tcctgcagct cgttcagcgc accgctcaga tcggttttca caaacagcac cggacgaccc
     1381 tgcgcgctca gacgaaacac cgccgcatca gagcagccaa tggtctgctg cgcccaatca
     1441 tagccaaaca gacgttccac ccacgctgcc gggctacccg catgcaggcc atcctgttca
     1501 atcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1621 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     1681 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1741 aagaatagac cgagataggg ttgagtggcc gctacagggc gctcccattc gccattcagg
     1801 ctgcgcaact gttgggaagg gcgtttcggt gcgggcctct tcgctattac gccagctggc
     1861 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1921 acgttgtaaa acgacggcca gtgagcgcga cgtaatacga ctcactatag ggcgaattga
     1981 aggaaggccg tcaaggccac gtgtcttgtc cgcggtacct acttgtaaac attaaatgtt
     2041 tatcttttca tgatatcaac ttgcgatcct gatgtgttaa taaaaaacct caagttctca
     2101 cttacagaaa cttttgtgtt atttcaccta atctttagga ttaatccttt tttcgtgagt
     2161 aatcttatcg cctcaggaga attacaatgg tttccaaggg cgaggaggat aacatggcta
     2221 tcattaaaga gttcatgcgc ttcaaagttc acatggaggg ttctgttaac ggtcacgagt
     2281 tcgagatcga aggcgaaggc gagggccgtc cgtatgaagg cacccagacc gccaaactga
     2341 aagtgactaa aggcggcccg ctgccttttg cgtgggacat cctgagcccg caatttatgt
     2401 acggttctaa agcgtatgtt aaacacccag cggatatccc ggactatctg aagctgtctt
     2461 ttccggaagg tttcaagtgg gaacgcgtaa tgaattttga agatggtggt gtcgtgaccg
     2521 tcactcagga ctcctccctg caggatggcg agttcatcta taaagttaaa ctgcgtggta
     2581 ctaattttcc atctgatggc ccggtgatgc agaaaaagac gatgggttgg gaggcgtcta
     2641 gcgaacgcat gtatccggaa gatggtgcgc tgaaaggcga aattaaacag cgcctgaaac
     2701 tgaaagatgg cggccattat gacgctgaag tgaaaaccac gtacaaagcc aagaaacctg
     2761 tgcagctgcc tggcgcgtac aatgtgaata ttaaactgga catcacctct cataatgaag
     2821 attatacgat cgtagagcaa tatgagcgcg cggagggtcg tcattctacc ggtggcatgg
     2881 atgagctgta caaataaaaa ggggctaccg gcgaaccagc agccccttta taaaggcgct
     2941 tcagtagtca gaccagcatc agtcctgaaa aggcgggcct gcgcccgcct ccaggttgct
     3001 acttaccgga ttcgtaagcc atgaaagccg ccacctccct gtgtccgtct ctgtaacgaa
     3061 tctcgcacag cgattttcgt gtcagataag tgaatatcaa cagtgtgaga cacacgatca
     3121 acacacacca gacaagggaa cttcgtggta gtttcatggc cttcttctcc ttgcgcaaag
     3181 cgcggtaaga ggctatcctg atgtggacta gacataggga tgcctcgtgg tggttaatga
     3241 aaattaactt actacggggc tatcttcttt ctgccacaca acacggcaac aaaccacctt
     3301 cacgtcatga ggcagaaagc ctcaagcgcc gggcacatca tagcccatat acctgcacgc
     3361 tgaccacact cactttccct gaaaataatc cgctcattca gaccgttcac gggaaatccg
     3421 tgtgattgtt gccgcatcac gctgcctccc ggagtttgtt cggcataagt cacaagagac
     3481 actcaatggc aaaggaagac cgtgccctgt tatccaggct tatggcagat tagcttcccg
     3541 gagagaaaac tgtcgaaaac tgacggtatg aacaccgtaa gctcccaaag tgatcaccat
     3601 tcgctttcat gcatagctat gcagcgagct gaaaacgatc ctgacgcatc cttcctgttt
     3661 ttccggggta aaacatctct ttttgcggtg tctgcgtcag aatcgcgttc agcgcgtttc
     3721 agtggtgcgt acaattaagg gattatggta aatatatgag ctatgcgata actttaactg
     3781 tgaagcgatg aacccattac aggcaaagcc aattactcct gacagtggtt tagccagaag
     3841 cagggctacc aagaccaatg caataagtaa tatatcgttt tgctatcgtg catcgctcgc
     3901 gctcagttcc attgtgcttt tttaagctgt cgtttttctt acggtatata ccggtttttt
     3961 atggcgtggt ttcttaactt gttcagctac tgatggaccc attgtatcta ggtagtcaac
     4021 tagctttgtt agatcataaa tattgcgacc accatatcgg cgatcactct tcgatgttgg
     4081 tttcttgtcc aagagattag ctttttcaag atcattgata gctctctgaa cagtccgtac
     4141 agaaaccccc atacgtatgg ctagactttc cattgacgga tgcggccact cttgcaaact
     4201 ccaccagtga acgatcaggt taagtagtgt gttaaaggcc actgaagtta gctttttctc
     4261 gttttgtata aaaaacaata cggtaggcac tgctgtccag ccaagagaca aaccgccagc
     4321 tttccattta ttcttaacgg agtaagtcat tgattttcct aagccccaaa atatttaaag
     4381 tatatattat atggtatatt catatgaata gggtgacact ggcgccatta ttgtgcaacc
     4441 aaaaaagact actctgaaaa cgaggaaaag attttttcct gcctgaatta gatacggagt
     4501 tagcgatatg aaaaccgaac aacgtcatga tcttgttaaa gatattgagg tttttggcgt
     4561 atccttgtct ctgttgattt ccagagcgaa tgagaagtct gttacaatgc catctggtct
     4621 aagtcgggag cagagaagag catgggcagc ggagcaggcg cgcaaaatcc acaattgaat
     4681 attgtctcat tctctgagac cttcaacctt tattacacat ccagatattc tgcaaaaaca
     4741 ctcgataaaa tcgatgattt cattgagcat tttgaaaaat acaatctctt tggcgatcct
     4801 ttaaaaggat atccagcttg gactggcaaa gtatcgccat cgtggaaagt gcctgatcat
     4861 tacgaaaaca aagaagctat tgagaagtat gctagagcta acaaattatg gcatgctcat
     4921 ttaggcgatc cggtttttaa agatacgttt catgggaaat acaaggtttc tgactgggtt
     4981 attcatttcc agcggctgac accgaaccat ataaagcttt tagagcttgg ataccatgac
     5041 ccaatgaagc tacctcaaaa ctttgaatga tcgagcggca ggctaaatga aatcttgaga
     5101 ttcattcagt ctcgtcgtaa tctcactatt gtaaaaacga aaaaaccacc ctggcaggtg
     5161 gtttttcgaa ggttagttaa tcctggcaga ttctctaacc gtggtaacag tcttgtgcga
     5221 gacatgtcac caaatactgt cctttcagtg tagcctcagt taggccgcca cttcaagaac
     5281 tctcgttaca tctctcgcac atcctgctta ccagtggccg ttgccagtgg cgttaagtcg
     5341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga accgagatac
     5461 caacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5641 tgctcgtcag gggggcggag cctatggaaa aacgcctccc gcgcgccctt cttctgggtc
     5701 tttgtctttt gctcacatgt tctttccggt tttatccccc g
//
