LOCUS       sequence-388623-        4416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ge Y, Paisie TK, Chen S, Concannon P
  TITLE     UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes.
  JOURNAL   J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 
            10.4049/jimmunol.1801338. Epub 2019 Oct 28.
  PUBMED    31659016
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801338"; journalName: "J Immunol"; date: 
            "2019-12-1- 1"; volume: "203"; issue: "11"; pages: "2827-2836"
COMMENT     Sequence Label: pDONR221-K202R-short-UBASH3A-Ctag
FEATURES             Location/Qualifiers
     source          1..4416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(259..286)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(378..464)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     513..535
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     527..544
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     528..544
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    560..659
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2532..2631)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2648..2667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2649..2667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2672..2688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2672..2688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2790..2809)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2801..3610
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2868..2887)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3757..4345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4246..4265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
       61 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      241 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      301 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
      361 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      421 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
      481 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
      541 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
      601 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctt
      661 catggcagcg ggggagacgc agctctacgc caaggtctcc aacaagctca agagccgcag
      721 cagcccctcg ctcctggagc ccctcctggc catgggcttc ccggtgcaca ccgcgctgaa
      781 agcgttggca gccacgggga ggaagacggc ggaggaggcc ttggcctggc tgcatgatca
      841 ttgcaatgac ccttccctag acgaccccat cccccaggag tatgcccttt tcctctgtcc
      901 aacggggccc ctgctggaaa aacttcaaga gttctggaga gagagcaagc gccagtgtgc
      961 aaagaacaga gctcatgagg tcttcccaca cgtgacactc tgtgacttct tcacgtgtga
     1021 agaccagaag gtggaatgcc tgtacgaggc gctgaagaga gctggagaca ggctcctggg
     1081 ctccttcccc acggccgtgc ctctggctct ccactcctcc atcagctacc tcggcttctt
     1141 cgtcagtggc agccccgcag acgtcatccg ggaattcgcc atgaccttcg ccacggaagc
     1201 atctctctta gcagactgct ccgtgaagcc ttgcaccaaa cagctgcatc tgaccttggc
     1261 ccaccggttc tacccccacc accagaggac gctggagcag ctggccagag ccatccccct
     1321 gggccacagc tgccagtgga ccgcagcact ctactcccga gacatgcgct ttgtgcacta
     1381 ccagaccctg agagccctat tccagtacaa accccagaac gtggatgagc tgacgctaag
     1441 tcctggtgac tacatctttg tggaccccac gcagcaggac gaagccagcg agggctgggt
     1501 gattgggatc tcacagcgga cgggctgccg gggcttcctg ccggaaaact acacggatcg
     1561 agccagtgag tctgacacgt gggtgaagca caggatgtac accttcagtc tagccacaga
     1621 cctgaactcc agaaaggatg gtgaagccag cagcagatgc agcggggaat ttcttccaca
     1681 aacggcaagg agtcttagca gcttacaggc cttgcaggct accgttgcaa ggaagagtgt
     1741 gctggtggtt cgccacgggg agagagtgga tcagatcttc gggaaggcat ggctgcagca
     1801 atgctccact cctgatggga aatactacag gccagacctg aatttcccct gcagtctgcc
     1861 cagacggagt cgtgggatca aagactttga aaacgatccc ccattatcat cgtgtggcat
     1921 tttccagtcc agaattgcag gggacgcgct actggacagt ggtatcagaa tcagctctgt
     1981 gtttgcctcc ccagccctcc gctgtgtgca gacggccaaa ctcatcctgg aagaactcaa
     2041 actggagaaa aaaatcaaga tacgagtgga acctggaatc tttgaatgga caaaatggga
     2101 agctggcaag accaccccaa ccctcatgag cctggaagag ctgaaagagg caaatttcaa
     2161 cattgacact gattacaggc ccgcgtttcc cctgtccgcc ctcatgccgg ccgagagcta
     2221 ccaggagtac atggacaggt gcacggcgag catggtgcaa atcgtcaaca cctgtccgca
     2281 ggacacgggt gtcatcctaa ttgtgagtca cggctccact ctggactcct gcacgcggcc
     2341 actgctcggg ctgccgcccc gggaatgtgg ggattttgcc caactcgtga gaaagatccc
     2401 ttccctgggc atgtgcttct gtgaagaaaa taaagaggaa ggaaaatggg agttggtgaa
     2461 cccaccggtg aagaccctga cccacggggc gaacgcagca tttaactgga ggaactggat
     2521 ctcaggcaac aacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2581 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2641 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2701 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2761 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2821 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2881 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2941 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3001 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     3061 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     3121 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3181 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     3241 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3301 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3361 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3421 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3481 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3541 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3601 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3661 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3901 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4381 cttttgctgg ccttttgctc acatgttctt tcctgc
//
