LOCUS       sequence-388631-        7342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Retinoic Acid Receptor-alpha and Human Retinoid X 
            Receptor-alpha in pcDNA3.1(+) plasmid expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Zolfaghari R, Mattie FJ, Wei CH, Chisholm DR, Whiting A, Ross AC
  TITLE     CYP26A1 gene promoter is a useful tool for reporting RAR-mediated 
            retinoid activity.
  JOURNAL   Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022.
            Epub 2019 Apr 27.
  PUBMED    31039331
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2019-07-15"; volume: "577:98-109. doi"; pages: " 
            10.1016/j.ab.2019.04.022. Epub 2019 Apr 27"
COMMENT     Sequence Label: pcDNA3.1-hRAR-alpha.hRXR-alpha
FEATURES             Location/Qualifiers
     source          1..7342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2406..2423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2412..2636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2682..3110
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2769..2788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2979..3000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3119..3139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3137..3434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     3366..3385
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      3461..3470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5015..5136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5052..5071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5106..5125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5185..5201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5185..5201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5198..5220)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5209..5225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5233..5239,5240..5257,5258..5263))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5278..5299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5416..5433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5587..6175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5667..5686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6346..7137,7138..7206))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6969..6988
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7207..7311)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg ccagcaacag cagctcctgc ccgacacctg ggggcgggca cctcaatggg
      961 tacccggtgc ctccctacgc cttcttcttc ccccctatgc tgggtggact ctccccgcca
     1021 ggcgctctga ccactctcca gcaccagctt ccagttagtg gatatagcac accatcccca
     1081 gccaccattg agacccagag cagcagttct gaagagatag tgcccagccc tccctcgcca
     1141 ccccctctac cccgcatcta caagccttgc tttgtctgtc aggacaagtc ctcaggctac
     1201 cactatgggg tcagcgcctg tgagggctgc aagggcttct tccgccgcag catccagaag
     1261 aacatggtgt acacgtgtca ccgggacaag aactgcatca tcaacaaggt gacccggaac
     1321 cgctgccagt actgccgact gcagaagtgc tttgaagtgg gcatgtccaa ggagtctgtg
     1381 agaaacgacc gaaacaagaa gaagaaggag gtgcccaagc ccgagtgctc tgagagctac
     1441 acgctgacgc cggaggtggg ggagctcatt gagaaggtgc gcaaagcgca ccaggaaacc
     1501 ttccctgccc tctgccagct gggcaaatac actacgaaca acagctcaga acaacgtgtc
     1561 tctctggaca ttgacctctg ggacaagttc agtgaactct ccaccaagtg catcattaag
     1621 actgtggagt tcgccaagca gctgcccggc ttcaccaccc tcaccatcgc cgaccagatc
     1681 accctcctca aggctgcctg cctggacatc ctgatcctgc ggatctgcac gcggtacacg
     1741 cccgagcagg acaccatgac cttctcggac gggctgaccc tgaaccggac ccagatgcac
     1801 aacgctggct tcggccccct caccgacctg gtctttgcct tcgccaacca gctgctgccc
     1861 ctggagatgg atgatgcgga gacggggctg ctcagcgcca tctgcctcat ctgcggagac
     1921 cgccaggacc tggagcagcc ggaccgggtg gacatgctgc aggagccgct gctggaggcg
     1981 ctaaaggtct acgtgcggaa gcggaggccc agccgccccc acatgttccc caagatgcta
     2041 atgaagatta ctgacctgcg aagcatcagc gccaaggggg ctgagcgggt gatcacgctg
     2101 aagatggaga tcccgggctc catgccgcct ctcatccagg aaatgttgga gaactcagag
     2161 ggcctggaca ctctgagcgg acagccgggg ggtggggggc gggacggggg tggcctggcc
     2221 cccccgccag gcagctgtag ccccagcctc agccccagct ccaacagaag cagcccggcc
     2281 acccactccc cgtgaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
     2341 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     2401 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     2461 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     2521 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     2581 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     2641 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     2701 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2761 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2821 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2881 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2941 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3001 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3061 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3121 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3181 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3241 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3361 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     3421 ggaggctttt ttggaggatc tgcagaattc actagtgatt gccaccatgg acaccaaaca
     3481 tttcctgccg ctcgatttct ccacccaggt gaactcctcc ctcacctccc cgacggggcg
     3541 aggctccatg gctgccccct cgctgcaccc gtccctgggg cctggcatcg gctccccggg
     3601 acagctgcat tctcccatca gcaccctgag ctcccccatc aacggcatgg gcccgccttt
     3661 ctcggtcatc agctccccca tgggccccca ctccatgtcg gtgcccacca cacccaccct
     3721 gggcttcagc actggcagcc cccagctcag ctcacctatg aaccccgtca gcagcagcga
     3781 ggacatcaag ccccccctgg gcctcaatgg cgtcctcaag gtccccgccc acccctcagg
     3841 aaacatggct tccttcacca agcacatctg cgccatctgc ggggaccgct cctcaggcaa
     3901 gcactatgga gtgtacagct gcgaggggtg caagggcttc ttcaagcgga cggtgcgcaa
     3961 ggacctgacc tacacctgcc gcgacaacaa ggactgcctg attgacaagc ggcagcggaa
     4021 ccggtgccag tactgccgct accagaagtg cctggccatg ggcatgaagc gggaagccgt
     4081 gcaggaggag cggcagcgtg gcaaggaccg gaacgagaat gaggtggagt cgaccagcag
     4141 cgccaacgag gacatgccgg tggagaggat cctggaggct gagctggccg tggagcccaa
     4201 gaccgagacc tacgtggagg caaacatggg gctgaacccc agctcgccga acgaccctgt
     4261 caccaacatt tgccaagcag ccgacaaaca gcttttcacc ctggtggagt gggccaagcg
     4321 gatcccacac ttctcagagc tgcccctgga cgaccaggtc atcctgctgc gggcaggctg
     4381 gaatgagctg ctcatcgcct ccttctccca ccgctccatc gccgtgaagg acgggatcct
     4441 cctggccacc gggctgcacg tccaccggaa cagcgcccac agcgcagggg tgggcgccat
     4501 ctttgacagg gtgctgacgg agcttgtgtc caagatgcgg gacatgcaga tggacaagac
     4561 ggagctgggc tgcctgcgcg ccatcgtcct ctttaaccct gactccaagg ggctctcgaa
     4621 cccggccgag gtggaggcgc tgagggagaa ggtctatgcg tccttggagg cctactgcaa
     4681 gcacaagtac ccagagcagc cgggaaggtt cgctaagctc ttgctccgcc tgccggctct
     4741 gcgctccatc gggctcaaat gcctggaaca tctcttcttc ttcaagctca tcggggacac
     4801 acccattgac accttcctta tggagatgct ggaggcgccg caccaaatga cttagttcga
     4861 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4921 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4981 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5041 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5101 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5161 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5221 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5281 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5341 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5401 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5761 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6001 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6301 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6361 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6421 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6481 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6541 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6601 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6661 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6721 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6781 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6841 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6901 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6961 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7021 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7081 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7141 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7201 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7261 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7321 ccgaaaagtg ccacctgacg tc
//
