LOCUS       sequence-38865-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High copy homologous recombination plasmid for gene targeting at the
            mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1538
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          232..364
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
       61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacgggatc cgccatggtg
      181 gcggaagttc ctataccttt tgaagaatag gaacttcgga ataggaactt cctgtggaga
      241 gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc aagagtcttc
      301 tctgtctcga caagcccagt ttctattggt ctccttaaac ctgtcttgta accttgatac
      361 ttacggcgcg ccgaggcagg ctttgcgctc ggtggcaccg atccccgccc gaagcgaccg
      421 cgggtgacaa ggaggagaag acggcgccgg gtccggcagg gcctggcggg agggggcagc
      481 ccgggcgtgg cgggggttgc gggggggtcc tggagaaacg cttccagcta aggggccgct
      541 tggaaccctc cggcccccgc gcggcccggg gccgcaattt ccgttttaat taaagcgctg
      601 ccgcctcggc ccccaggacc ccgcccccgc ccctcttatc gtcccatcgc aataaagtct
      661 ccaacgcgcc gcgccacagc caagcgcacc cggcagcccc ccgcgccccg cagccgggga
      721 gacgcgcggg ggatgggtcc gggccatgtg ccctgatggg agcctagagc gcgtgtcttt
      781 tccgcctccc tgactctctg caaacagtga tggggctggt gtacccggcc ccacctcgga
      841 ccccagcagc cacagccccc tccct
//
