LOCUS       sequence-388659-        4293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 
            (for C-terminal tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  TITLE     Concannon Lab Plasmids
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-deltaUBA-short-UBASH3A-Ctag
FEATURES             Location/Qualifiers
     source          1..4293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2418..2517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2534..2553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2535..2553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2558..2574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2558..2574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2676..2695)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2687..3496
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2754..2773)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3643..4231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4132..4151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atggcagcgg gggagacgca gctctacgcc aaggtctcca acaagctcaa
      721 gagccgctgc aatgaccctt ccctagacga ccccatcccc caggagtatg cccttttcct
      781 ctgtccaacg gggcccctgc tggaaaaact tcaagagttc tggagagaga gcaagcgcca
      841 gtgtgcaaag aacagagctc atgaggtctt cccacacgtg acactctgtg acttcttcac
      901 gtgtgaagac cagaaggtgg aatgcctgta cgaggcgctg aagagagctg gagacaggct
      961 cctgggctcc ttccccacgg ccgtgcctct ggctctccac tcctccatca gctacctcgg
     1021 cttcttcgtc agtggcagcc ccgcagacgt catccgggaa ttcgccatga ccttcgccac
     1081 ggaagcatct ctcttagcag actgctccgt gaagccttgc accaaacagc tgcatctgac
     1141 cttggcccac aagttctacc cccaccacca gaggacgctg gagcagctgg ccagagccat
     1201 ccccctgggc cacagctgcc agtggaccgc agcactctac tcccgagaca tgcgctttgt
     1261 gcactaccag accctgagag ccctattcca gtacaaaccc cagaacgtgg atgagctgac
     1321 gctaagtcct ggtgactaca tctttgtgga ccccacgcag caggacgaag ccagcgaggg
     1381 ctgggtgatt gggatctcac agcggacggg ctgccggggc ttcctgccgg aaaactacac
     1441 ggatcgagcc agtgagtctg acacgtgggt gaagcacagg atgtacacct tcagtctagc
     1501 cacagacctg aactccagaa aggatggtga agccagcagc agatgcagcg gggaatttct
     1561 tccacaaacg gcaaggagtc ttagcagctt acaggccttg caggctaccg ttgcaaggaa
     1621 gagtgtgctg gtggttcgcc acggggagag agtggatcag atcttcggga aggcatggct
     1681 gcagcaatgc tccactcctg atgggaaata ctacaggcca gacctgaatt tcccctgcag
     1741 tctgcccaga cggagtcgtg ggatcaaaga ctttgaaaac gatcccccat tatcatcgtg
     1801 tggcattttc cagtccagaa ttgcagggga cgcgctactg gacagtggta tcagaatcag
     1861 ctctgtgttt gcctccccag ccctccgctg tgtgcagacg gccaaactca tcctggaaga
     1921 actcaaactg gagaaaaaaa tcaagatacg agtggaacct ggaatctttg aatggacaaa
     1981 atgggaagct ggcaagacca ccccaaccct catgagcctg gaagagctga aagaggcaaa
     2041 tttcaacatt gacactgatt acaggcccgc gtttcccctg tccgccctca tgccggccga
     2101 gagctaccag gagtacatgg acaggtgcac ggcgagcatg gtgcaaatcg tcaacacctg
     2161 tccgcaggac acgggtgtca tcctaattgt gagtcacggc tccactctgg actcctgcac
     2221 gcggccactg ctcgggctgc cgccccggga atgtggggat tttgcccaac tcgtgagaaa
     2281 gatcccttcc ctgggcatgt gcttctgtga agaaaataaa gaggaaggaa aatgggagtt
     2341 ggtgaaccca ccggtgaaga ccctgaccca cggggcgaac gcagcattta actggaggaa
     2401 ctggatctca ggcaacaacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2461 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2521 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2581 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2641 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2701 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2761 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2821 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2881 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2941 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     3001 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3061 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3121 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3181 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     3241 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3301 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3361 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3421 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3481 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3541 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3781 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4021 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4261 cctggccttt tgctggcctt ttgctcacat gtt
//
