LOCUS       sequence-38866-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Linda Hicke lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP1034 - ECFP-Nterm
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(360..379)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(361..379)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(386..403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(386..402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(630..649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 ctgaagcact gcacgcccca ggtcagggtg gccacgaggg tgggccaggg cacgggcagc
      121 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      181 tcgccggaca cgctgaactc gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      241 accaccccgg tgaacagctc ctcgcccttg ctcaccatgc ggccgcgttt aagggcgaat
      301 tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg cccaattcgc
      361 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      541 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      601 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      721 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      841 tggnctcttg ttccaaactg gnacaacact caaccctatc tc
//
