LOCUS       sequence-388662-        4416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ge Y, Paisie TK, Chen S, Concannon P
  TITLE     UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes.
  JOURNAL   J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 
            10.4049/jimmunol.1801338. Epub 2019 Oct 28.
  PUBMED    31659016
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801338"; journalName: "J Immunol"; date: 
            "2019-12-1- 1"; volume: "203"; issue: "11"; pages: "2827-2836"
COMMENT     Sequence Label: pDONR221-W279A-short-UBASH3A-Ctag
FEATURES             Location/Qualifiers
     source          1..4416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2541..2640)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2657..2676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2658..2676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2681..2697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2681..2697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2799..2818)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2810..3619
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2877..2896)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3766..4354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4255..4274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atggcagcgg gggagacgca gctctacgcc aaggtctcca acaagctcaa
      721 gagccgcagc agcccctcgc tcctggagcc cctcctggcc atgggcttcc cggtgcacac
      781 cgcgctgaaa gcgttggcag ccacggggag gaagacggcg gaggaggcct tggcctggct
      841 gcatgatcat tgcaatgacc cttccctaga cgaccccatc ccccaggagt atgccctttt
      901 cctctgtcca acggggcccc tgctggaaaa acttcaagag ttctggagag agagcaagcg
      961 ccagtgtgca aagaacagag ctcatgaggt cttcccacac gtgacactct gtgacttctt
     1021 cacgtgtgaa gaccagaagg tggaatgcct gtacgaggcg ctgaagagag ctggagacag
     1081 gctcctgggc tccttcccca cggccgtgcc tctggctctc cactcctcca tcagctacct
     1141 cggcttcttc gtcagtggca gccccgcaga cgtcatccgg gaattcgcca tgaccttcgc
     1201 cacggaagca tctctcttag cagactgctc cgtgaagcct tgcaccaaac agctgcatct
     1261 gaccttggcc cacaagttct acccccacca ccagaggacg ctggagcagc tggccagagc
     1321 catccccctg ggccacagct gccagtggac cgcagcactc tactcccgag acatgcgctt
     1381 tgtgcactac cagaccctga gagccctatt ccagtacaaa ccccagaacg tggatgagct
     1441 gacgctaagt cctggtgact acatctttgt ggaccccacg cagcaggacg aagccagcga
     1501 gggcgcggtg attgggatct cacagcggac gggctgccgg ggcttcctgc cggaaaacta
     1561 cacggatcga gccagtgagt ctgacacgtg ggtgaagcac aggatgtaca ccttcagtct
     1621 agccacagac ctgaactcca gaaaggatgg tgaagccagc agcagatgca gcggggaatt
     1681 tcttccacaa acggcaagga gtcttagcag cttacaggcc ttgcaggcta ccgttgcaag
     1741 gaagagtgtg ctggtggttc gccacgggga gagagtggat cagatcttcg ggaaggcatg
     1801 gctgcagcaa tgctccactc ctgatgggaa atactacagg ccagacctga atttcccctg
     1861 cagtctgccc agacggagtc gtgggatcaa agactttgaa aacgatcccc cattatcatc
     1921 gtgtggcatt ttccagtcca gaattgcagg ggacgcgcta ctggacagtg gtatcagaat
     1981 cagctctgtg tttgcctccc cagccctccg ctgtgtgcag acggccaaac tcatcctgga
     2041 agaactcaaa ctggagaaaa aaatcaagat acgagtggaa cctggaatct ttgaatggac
     2101 aaaatgggaa gctggcaaga ccaccccaac cctcatgagc ctggaagagc tgaaagaggc
     2161 aaatttcaac attgacactg attacaggcc cgcgtttccc ctgtccgccc tcatgccggc
     2221 cgagagctac caggagtaca tggacaggtg cacggcgagc atggtgcaaa tcgtcaacac
     2281 ctgtccgcag gacacgggtg tcatcctaat tgtgagtcac ggctccactc tggactcctg
     2341 cacgcggcca ctgctcgggc tgccgccccg ggaatgtggg gattttgccc aactcgtgag
     2401 aaagatccct tccctgggca tgtgcttctg tgaagaaaat aaagaggaag gaaaatggga
     2461 gttggtgaac ccaccggtga agaccctgac ccacggggcg aacgcagcat ttaactggag
     2521 gaactggatc tcaggcaaca acccagcttt cttgtacaaa gttggcatta taagaaagca
     2581 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2641 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2701 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2761 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2821 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2881 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2941 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3001 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3061 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     3121 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3181 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3241 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3301 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3361 gccgttctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3421 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3481 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3541 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3601 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3661 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3721 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3781 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3841 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3901 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3961 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4021 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4081 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4141 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4201 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4261 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4321 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4381 gttcctggcc ttttgctggc cttttgctca catgtt
//
