LOCUS       sequence-388680-        7301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMUT2 based vector for sensing human primary bile acids in E. coli 
            Nissle 1917.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Vaaben TH, Vazquez-Uribe R, Sommer MOA
  TITLE     Characterization of Eight Bacterial Biosensors for Microbial 
            Diagnostic and Therapeutic Applications.
  JOURNAL   ACS Synth Biol. 2022 Dec 16;11(12):4184-4192. doi: 
            10.1021/acssynbio.2c00491. Epub 2022 Nov 30.
  PUBMED    36449712
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00491"; journalName: "ACS Synth Biol"; date: 
            "2022-12-16- 16"; volume: "11"; issue: "12"; pages: "4184-4192"
COMMENT     Sequence Label: pMUT2-pTcpP-mCherry
FEATURES             Location/Qualifiers
     source          1..7301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..88
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     80..97
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     81..97
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     108..127
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        108..126
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             131..142
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(184..211)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(227..298)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5286..5305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5528..5545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(6167..6961)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6962..7066)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
       61 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgacgtaa tacgactcac
      121 tatagggcga attgaaggaa ggccgtcaag gccacgtgtc ttgtccgcgg tacctactat
      181 aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg
      241 acaaacaaca gataaaacga aaggcccagt ttttcgactg agcctttcgt tttatttgat
      301 gcctggttag aaatcgctct gacacgagaa ccacatcttt tccgccgtca gcaatttgta
      361 aggaaaagcc agaaagcacc ctttgccaag ttgatagatc gacaatacat tgtaatcacg
      421 agaagcattc gattgggagt aatcggggat cagattcagc ttttgtgatg aagggtcagg
      481 caatgtttgg tagcgtgtag tccatgattt aacaacatta ccttgagggt ccgtctgagt
      541 aagccacaca ttagacagat tgccctggta ggtgctaaag atttgagtaa cggttccatc
      601 aataatcatg gtcccgtggc tatttttctg gcgcatagga agagcaataa ttgttacccc
      661 cagggcgacc acaaacagac cagtggcccc tccccaagcc tttaacttct tgtgcattac
      721 tttacatttt cttaataata actactccac ataaattacg agccggatga ttaattgtca
      781 atttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
      841 aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccggtttatt
      901 agttcttagt acattccaac gtcttatcag aaaaaacaca ttgatgctgg tcgatagcaa
      961 aggtcaactt ctgctcgcta agtttctgga ttgtgattcc aggaagacgg gctaagtcac
     1021 gtaaatcgtg agtaatcccg gccaattcgt gttgatacca ttgcccgtat gaccaccaaa
     1081 tgacaggtaa taaggcaaca tataacgctg aaaacaccag atacggcacc actcgtttgc
     1141 ttttaactgg ggattgttca ggtggcgttg cggtgttttg aatgttaaga ctgtgggagg
     1201 gagaatctgt ggcaggaacc gcctctggta tagggggagg cgaagatagc attatttcct
     1261 ctccctcttc ttcgctgtac cagataaccg gcaccattaa tttatagccg cgctttggta
     1321 cagtagcgat atagacagga ctatcttcat cattatcttt taatgactta cgtagttctg
     1381 agatactctg cgtcacaacg tgattggtga caatacttct cttccagaca ttatcgataa
     1441 gttcatccct gctaagtact tcgccactgt gttgagcaaa gaaaaccaga agatcgatta
     1501 atctcggctc aagggtaagt tgacgcccat tgcggctaat ttggtttatg gacggagtaa
     1561 caagccattc gccaacgcga actacaggtt gttgcatatg tcttctgttc ataattcacg
     1621 gaactccaca cattatacga gccgatgatt aagaggcaag aaataagatc actaccgggc
     1681 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatcca ttgtaaacat
     1741 taaatgttta tcttttcatg atatcaactt gcgatcctga tgtgttaata aaaaacctca
     1801 agttctcact tacagaaact tttgtgttat ttcacctaat ctttaggatt aatccttttt
     1861 tcgtgagtaa tcttatcgcc agtttggtca ggagaattac aatggtttcc aagggcgagg
     1921 aggataacat ggctatcatt aaagagttca tgcgcttcaa agttcacatg gagggttctg
     1981 ttaacggtca cgagttcgag atcgaaggcg aaggcgaggg ccgtccgtat gaaggcaccc
     2041 agaccgccaa actgaaagtg actaaaggcg gcccgctgcc ttttgcgtgg gacatcctga
     2101 gcccgcaatt tatgtacggt tctaaagcgt atgttaaaca cccagcggat atcccggact
     2161 atctgaagct gtcttttccg gtaggtttca agtgggaacg cgtaatgaat tttgaagatg
     2221 gtggtgtcgt gaccgtcact caggactcct ccctgcagga tggcgagttc atctataaag
     2281 ttaaactgcg tggtactaat tttccatctg atggcccggt gatgcagaaa aagacgatgg
     2341 gttgggaggc gtctagcgaa cgcatgtatc cggaagatgg tgcgctgaaa ggcgaaatta
     2401 aacagcgcct gaaactgaaa gatggcggcc attatgacgc tgaagtgaaa accacgtaca
     2461 aagccaagaa acctgtgcag ctgcctggcg cgtacaatgt gaatattaaa ctggacatca
     2521 cctctcataa tgaagattat acgatcgtag agcaatatga gcgcgcggag ggtcgtcatt
     2581 ctaccggtgg catggatgag ctgtacaaat aaaaaggggc taccggcgaa ccagcagccc
     2641 ctttataaag gcgcttcagt agtcagacca gcatcagtcc tgaaaaggcg ggcctgcgcc
     2701 cgcctccagg ttgctactta ccggattcgt aagccatgaa agccgccacc tccctgtgtc
     2761 cgtctctgta acgaatctcg cacagcgatt ttcgtgtcgg ataagtgaat atcaacagtg
     2821 tgagacacac gatcaacaca caccagacaa gggaacttcg tggtagtttc atggccttct
     2881 tctccttgcg caaagcgcgg taagaggcta tcctgatgtg gactagacat agggatgcct
     2941 cgtggtggtt aatgaaaatt aacttactac ggggctatct tctttctgcc acacaacacg
     3001 gcaacaaacc accttcacgt catgaggcag aaagcctcaa gcgccgggca catcatagcc
     3061 catatacctg cacgctgacc acactcactt tccctgaaaa taatccgctc attcagaccg
     3121 ttcacgggaa atccgtgtga ttgttgccgc atcacgctgc ctcccggagt ttgttcggca
     3181 taagtcacaa gagacactca atggcaaagg aagaccgtgc cctgttatcc aggcttatgg
     3241 cagattagct tcccggagag aaaactgtcg aaaactgacg gtatgaacac cgtaagctcc
     3301 caaagtgatc accattcgct ttcatgcata gctatgcagc gagctgaaaa cgatcctgac
     3361 gcatccttcc tgtttttccg gggtaaaaca tctctttttg cggtgtctgc gtcagaatcg
     3421 cgttcagcgc gtttcagtgg tgcgtacaat taagggatta tggtaaatat atgagctatg
     3481 cgataacttt aactgtgaag cgatgaaccc attacaggca aagccaatta ctcctgacag
     3541 tggtttagcc agaagcaggg ctaccaagac caatgcaata agtaatatat cgttttgcta
     3601 tcgtgcatcg ctcgcgctca gttccattgt gcttttttaa gctgtcgttt ttcttacggt
     3661 atataccggt tttttatggc gtggtttctt aacttgttca gctactgatg gacccattgt
     3721 atctaggtag tcaactagct ttgttagatc ataaatattg cgaccaccat atcggcgatc
     3781 actcttcgat gttggtttct tgtccaagag attagctttt tcaagatcat tgatagctct
     3841 ctgaacagtc cgtacagaaa cccccatacg tatggctaga ctttccattg acggatgcgg
     3901 ccactcttgc aaactccacc agtgaacgat caggttaagt agtgtgttaa aggccactga
     3961 agttagcttt ttctcgtttt gtataaaaaa caatacggta ggcactgctg tccagccaag
     4021 agacaaaccg ccagctttcc atttattctt aacggagtaa gtcattgatt ttcctaagcc
     4081 ccaaaatatt taaagtatat attatatggt atattcatat gaatagggtg acactggcgc
     4141 cattattgtg caaccaaaaa agactactct gaaaacgagg aaaagatttt ttcctgcctg
     4201 aattagatac ggagttagcg atatgaaaac cgaacaacgt catgatcttg ttaaagatat
     4261 tgaggttttt ggcgtatcct tgtctctgtt gatttccaga gcgaatgaga agtctgttac
     4321 aatgccatct ggtctaagtc gggagcagag aagagcatgg gcagcggagc aggcgcgcaa
     4381 aatccacaat tgaatattgt ctcattctct gagaccttca acctttatta cacatccaga
     4441 tattctgcaa aaacactcga taaaatcgat gatttcattg agcattttga aaaatacaat
     4501 ctctttggcg atcctttaaa aggatatcca gcttggactg gcaaagtatc gccatcgtgg
     4561 aaagtgcctg atcattacga aaacaaagaa gctattgaga agtatgctag agctaacaaa
     4621 ttatggcatg ctcatttagg cgatccggtt tttaaagata cgtttcatgg gaaatacaag
     4681 gtttctgact gggttattca tttccagcgg ctgacaccga accatataaa gcttttagag
     4741 cttggatacc atgacccaat gaagctacct caaaactttg aatgatcgag cggcaggcta
     4801 aatgaaatct tgagattcat tcagtctcgt cgtaatctca ctattgtaaa aacgaaaaaa
     4861 ccaccctggc aggtggtttt tcgaaggtta gttaatcctg gcagattctc taaccgtggt
     4921 aacagtcttg tgcgagacat gtcaccaaat actgtccttt cagtgtagcc tcagttaggc
     4981 cgccacttca agaactctcg ttacatctct cgcacatcct gcttaccagt ggccgttgcc
     5041 agtggcgtta agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5161 accgaaccga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5341 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc ctcccgcgcg
     5401 cccttcttct gggtctttgt cttttgctca catgttcttt ccggttttat cccccgattc
     5461 tgtggataac cgtattaccg cctttgagtg agctgacacc gctcaccgca gtcgaacgac
     5521 cgagcgtagc gagtcagtga gcgaggaagc ggaagagcgc cttatgtgac attttctcct
     5581 tacgctcctc tatgccgttc tgcatcctgt ccggatgcgt tatatcccgg taagattttc
     5641 cgcttcaaag cgtgtctgta tgctgttctg gagttcttct gcgagttcgt gcagtttctc
     5701 acacatggcg gcctgttcgt cggcattgag tgcgtccagt ttttcgagca gcgtcaggct
     5761 ctgacttttt atgaatcccg ccatgttgag tacggcttgc tgctgcttat tcatcttttc
     5821 gttttctccg ttctgtctgt catctgcgtt gtgtgattat atcgcgtacc acttttcgac
     5881 tgttttgctg ccgctattct gccgcttggc tttttgacgg gcatttctgt cagacaacac
     5941 tgtcactgcc aaaaaactgc cgtgcctttg tcggtaattc gagcttgctg acaggacagg
     6001 atgtgcaatt gttataccgc gcatacatgc acgctattac aattgcccgt taagggattt
     6061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttattag aaaaattcat
     6181 ccagcagacg ataaaacgca atacgctggc tatccggtgc cgcaatgcca tacagcacca
     6241 gaaaacgatc cgcccattcg ccgcccagtt cttccgcaat atcacgggtg gccagcgcaa
     6301 tatcctgata acgatccgcc acgcccagac ggccgcaatc aataaagccg ctaaaacggc
     6361 cattttccac cataatgttc ggcaggcacg catcaccatg ggtcaccacc agatcttcgc
     6421 catccggcat gctcgctttc agacgcgcaa acagctctgc cggtgccagg ccctgatgtt
     6481 cttcatccag atcatcctga tccaccaggc ccgcttccat acgggtacgc gcacgttcaa
     6541 tacgatgttt cgcctgatga tcaaacggac aggtcgccgg gtccagggta tgcagacgac
     6601 gcatggcatc cgccataatg ctcacttttt ctgccggcgc cagatggcta gacagcagat
     6661 cctgacccgg cacttcgccc agcagcagcc aatcacggcc cgcttcggtc accacatcca
     6721 gcaccgccgc acacggaaca ccggtggtgg ccagccagct cagacgcgcc gcttcatcct
     6781 gcagctcgtt cagcgcaccg ctcagatcgg ttttcacaaa cagcaccgga cgaccctgcg
     6841 cgctcagacg aaacaccgcc gcatcagagc agccaatggt ctgctgcgcc caatcatagc
     6901 caaacagacg ttccacccac gctgccgggc tacccgcatg caggccatcc tgttcaatca
     6961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7081 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     7141 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     7201 atagaccgag atagggttga gtggccgcta cagggcgctc ccattcgcca ttcaggctgc
     7261 gcaactgttg ggaagggcgt ttcggtgcgg gcctcttcgc t
//
