LOCUS       sequence-388683-        7509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMUT2 based vector for sensing human secondary bile acids in E. coli
            Nissle 1917.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Vaaben TH, Vazquez-Uribe R, Sommer MOA
  TITLE     Characterization of Eight Bacterial Biosensors for Microbial 
            Diagnostic and Therapeutic Applications.
  JOURNAL   ACS Synth Biol. 2022 Dec 16;11(12):4184-4192. doi: 
            10.1021/acssynbio.2c00491. Epub 2022 Nov 30.
  PUBMED    36449712
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00491"; journalName: "ACS Synth Biol"; date: 
            "2022-12-16- 16"; volume: "11"; issue: "12"; pages: "4184-4192"
COMMENT     Sequence Label: pMUT2-VtrA-mCherry
FEATURES             Location/Qualifiers
     source          1..7509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1067..1086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1309..1326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1948..2742)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2743..2847)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3148..3170
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3162..3179
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3163..3179
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3190..3209
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3190..3208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3213..3224
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3266..3293)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3309..3380)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gatatgaaaa ccgaacaacg tcatgatctt gttaaagata ttgaggtttt tggcgtatcc
       61 ttgtctctgt tgatttccag agcgaatgag aagtctgtta caatgccatc tggtctaagt
      121 cgggagcaga gaagagcatg ggcagcggag caggcgcgca aaatccacaa ttgaatattg
      181 tctcattctc tgagaccttc aacctttatt acacatccag atattctgca aaaacactcg
      241 ataaaatcga tgatttcatt gagcattttg aaaaatacaa tctctttggc gatcctttaa
      301 aaggatatcc agcttggact ggcaaagtat cgccatcgtg gaaagtgcct gatcattacg
      361 aaaacaaaga agctattgag aagtatgcta gagctaacaa attatggcat gctcatttag
      421 gcgatccggt ttttaaagat acgtttcatg ggaaatacaa ggtttctgac tgggttattc
      481 atttccagcg gctgacaccg aaccatataa agcttttaga gcttggatac catgacccaa
      541 tgaagctacc tcaaaacttt gaatgatcga gcggcaggct aaatgaaatc ttgagattca
      601 ttcagtctcg tcgtaatctc actattgtaa aaacgaaaaa accaccctgg caggtggttt
      661 ttcgaaggtt agttaatcct ggcagattct ctaaccgtgg taacagtctt gtgcgagaca
      721 tgtcaccaaa tactgtcctt tcagtgtagc ctcagttagg ccgccacttc aagaactctc
      781 gttacatctc tcgcacatcc tgcttaccag tggccgttgc cagtggcgtt aagtcgtgtc
      841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaaccg agataccaac
      961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1141 cgtcaggggg gcggagccta tggaaaaacg cctcccgcgc gcccttcttc tgggtctttg
     1201 tcttttgctc acatgttctt tccggtttta tcccccgatt ctgtggataa ccgtattacc
     1261 gcctttgagt gagctgacac cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg
     1321 agcgaggaag cggaagagcg ccttatgtga cattttctcc ttacgctcct ctatgccgtt
     1381 ctgcatcctg tccggatgcg ttatatcccg gtaagatttt ccgcttcaaa gcgtgtctgt
     1441 atgctgttct ggagttcttc tgcgagttcg tgcagtttct cacacatggc ggcctgttcg
     1501 tcggcattga gtgcgtccag tttttcgagc agcgtcaggc tctgactttt tatgaatccc
     1561 gccatgttga gtacggcttg ctgctgctta ttcatctttt cgttttctcc gttctgtctg
     1621 tcatctgcgt tgtgtgatta tatcgcgtac cacttttcga ctgttttgct gccgctattc
     1681 tgccgcttgg ctttttgacg ggcatttctg tcagacaaca ctgtcactgc caaaaaactg
     1741 ccgtgccttt gtcggtaatt cgagcttgct gacaggacag gatgtgcaat tgttataccg
     1801 cgcatacatg cacgctatta caattgcccg ttaagggatt ttggtcatga gattatcaaa
     1861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1921 atatgagtaa acttggtctg acagttatta gaaaaattca tccagcagac gataaaacgc
     1981 aatacgctgg ctatccggtg ccgcaatgcc atacagcacc agaaaacgat ccgcccattc
     2041 gccgcccagt tcttccgcaa tatcacgggt ggccagcgca atatcctgat aacgatccgc
     2101 cacgcccaga cggccgcaat caataaagcc gctaaaacgg ccattttcca ccataatgtt
     2161 cggcaggcac gcatcaccat gggtcaccac cagatcttcg ccatccggca tgctcgcttt
     2221 cagacgcgca aacagctctg ccggtgccag gccctgatgt tcttcatcca gatcatcctg
     2281 atccaccagg cccgcttcca tacgggtacg cgcacgttca atacgatgtt tcgcctgatg
     2341 atcaaacgga caggtcgccg ggtccagggt atgcagacga cgcatggcat ccgccataat
     2401 gctcactttt tctgccggcg ccagatggct agacagcaga tcctgacccg gcacttcgcc
     2461 cagcagcagc caatcacggc ccgcttcggt caccacatcc agcaccgccg cacacggaac
     2521 accggtggtg gccagccagc tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc
     2581 gctcagatcg gttttcacaa acagcaccgg acgaccctgc gcgctcagac gaaacaccgc
     2641 cgcatcagag cagccaatgg tctgctgcgc ccaatcatag ccaaacagac gttccaccca
     2701 cgctgccggg ctacccgcat gcaggccatc ctgttcaatc atactcttcc tttttcaata
     2761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     2881 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2941 caataggcct aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3001 agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3121 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3181 agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca aggccacgtg
     3241 tcttgtccgc ggtacctact ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
     3301 ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     3361 tgagcctttc gttttatttg atgcctggtt attaatagcg atgcaatgtg ataatctgtc
     3421 catcataaaa gaagaactct accacaacat actcttccga taagtaaaca acgcgaatgt
     3481 tatttgactt gttgatgagc gaggagatgc tagtcggttc cttatacggc ttcatgttcg
     3541 ctttattagt acccgtgtaa acgtcgatgt tatcgaagtt caagtagtat tgatgggtgt
     3601 tcttttgaat gatgtctcca tgtccaataa cgttatagta attcttgttc tctgacttca
     3661 ccagctcgcg gatgatgata tctgaggtga agtggttgcc cttaatcgag acattgatcg
     3721 cgacgtcgtg catgtacgtc gagtccgccg cttggtactt gtatgacgtt tcgtagaagt
     3781 gcacgggttg aaagaaatta ttgtagatga ttaccagcag catgacgaca ctcatcaggg
     3841 tcaggctcaa atgcagacgc ttaatattaa gtttcattta caatatattc actaatatgc
     3901 ttatatccac ataaattacg agccggatga ttaattgtca atttcttttg ggtatagcgt
     3961 cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt
     4021 tacgtaaatc aggtgaaact gaccgataag ccggttatta gtactcaatc tcattgacga
     4081 tgtactcgga gatatgctta taattgagtt tcttttccgt gcttaatacg cgattcttgg
     4141 acttcttatt cttgctgata aaactgaacg agatggtctt gtcattcaac ataacgtaaa
     4201 gacgctcggg tacaatttct tttgcactta aagcgttgat aagggccacg cccagctcct
     4261 cgcttaattg aaagtctgaa gaaaagatgt agacgtagtc ttgttggaac gataaactgg
     4321 gatagtcgtc ctttgcggtg gacgacacga aaatactggt gtagctataa gccacataga
     4381 tgatgaaagc caactgcaaa ataatgctga taagaagaat tcgtttgctt ttaactgggg
     4441 attgttcagg tggcgttgcg gtgttttgaa tgttaagact gtgggaggga gaatctgtgg
     4501 caggaaccgc ctctggtata gggggaggcg aagatagcat tatttcctct ccctcttctt
     4561 cgctgtacca gataaccggc accattaatt tatagccgcg ctttggtaca gtagcgatat
     4621 agacaggact atcttcatca ttatctttta atgacttacg tagttctgag atactctgcg
     4681 tcacaacgtg attggtgaca atacttctct tccagacatt atcgataagt tcatccctgc
     4741 taagtacttc gccactgtgt tgagcaaaga aaaccagaag atcgattaat ctcggctcaa
     4801 gggtaagttg acgcccattg cggctaattt ggtttatgga cggagtaaca agccattcgc
     4861 caacgcgaac tacaggttgt tgcatttcac atctacctgc tgttgtatgg tgtctccaca
     4921 cattatacga gccgatgatt aagaggcaag aaataagatc actaccgggc gtattttttg
     4981 agttatcgag attttcagga gctaaggaag ctaaaatcca ttgtaaacat taaatgttta
     5041 tcttttcatg atatcaactt gcgatcctga tgtgttaata aaaaacctca agttctcact
     5101 tacagaaact tttgtgttat ttcacctaat ctttaggatt aatccttttt tcgtgagtaa
     5161 tcttatcgcc agtttggtca ggagaattac aatggtttcc aagggcgagg aggataacat
     5221 ggctatcatt aaagagttca tgcgcttcaa agttcacatg gagggttctg ttaacggtca
     5281 cgagttcgag atcgaaggcg aaggcgaggg ccgtccgtat gaaggcaccc agaccgccaa
     5341 actgaaagtg actaaaggcg gcccgctgcc ttttgcgtgg gacatcctga gcccgcaatt
     5401 tatgtacggt tctaaagcgt atgttaaaca cccagcggat atcccggact atctgaagct
     5461 gtcttttccg gaaggtttca agtgggaacg cgtaatgaat tttgaagatg gtggtgtcgt
     5521 gaccgtcact caggactcct ccctgcagga tggcgagttc atctataaag ttaaactgcg
     5581 tggtactaat tttccatctg atggcccggt gatgcagaaa aagacgatgg gttgggaggc
     5641 gtctagcgaa cgcatgtatc cggaagatgg tgcgctgaaa ggcgaaatta aacagcgcct
     5701 gaaactgaaa gatggcggcc attatgacgc tgaagtgaaa accacgtaca aagccaagaa
     5761 acctgtgcag ctgcctggcg cgtacaatgt gaatattaaa ctggacatca cctctcataa
     5821 tgaagattat acgatcgtag agcaatatga gcgcgcggag ggtcgtcatt ctaccggtgg
     5881 catggatgag ctgtacaaat aaaaaggggc taccggcgaa ccagcagccc ctttataaag
     5941 gcgcttcagt agtcagacca gcatcagtcc tgaaaaggcg ggcctgcgcc cgcctccagg
     6001 ttgctactta ccggattcgt aagccatgaa agccgccacc tccctgtgtc cgtctctgta
     6061 acgaatctcg cacagcgatt ttcgtgtcag ataagtgaat atcaacagtg tgagacacac
     6121 gatcaacaca caccagacaa gggaacttcg tggtagtttc atggccttct tctccttgcg
     6181 caaagcgcgg taagaggcta tcctgatgtg gactagacat agggatgcct cgtggtggtt
     6241 aatgaaaatt aacttactac ggggctatct tctttctgcc acacaacacg gcaacaaacc
     6301 accttcacgt catgaggcag aaagcctcaa gcgccgggca catcatagcc catatacctg
     6361 cacgctgacc acactcactt tccctgaaaa taatccgctc attcagaccg ttcacgggaa
     6421 atccgtgtga ttgttgccgc atcacgctgc ctcccggagt ttgttcggca taagtcacaa
     6481 gagacactca atggcaaagg aagaccgtgc cctgttatcc aggcttatgg cagattagct
     6541 tcccggagag aaaactgtcg aaaactgacg gtatgaacac cgtaagctcc caaagtgatc
     6601 accattcgct ttcatgcata gctatgcagc gagctgaaaa cgatcctgac gcatccttcc
     6661 tgtttttccg gggtaaaaca tctctttttg cggtgtctgc gtcagaatcg cgttcagcgc
     6721 gtttcagtgg tgcgtacaat taagggatta tggtaaatat atgagctatg cgataacttt
     6781 aactgtgaag cgatgaaccc attacaggca aagccaatta ctcctgacag tggtttagcc
     6841 agaagcaggg ctaccaagac caatgcaata agtaatatat cgttttgcta tcgtgcatcg
     6901 ctcgcgctca gttccattgt gcttttttaa gctgtcgttt ttcttacggt atataccggt
     6961 tttttatggc gtggtttctt aacttgttca gctactgatg gacccattgt atctaggtag
     7021 tcaactagct ttgttagatc ataaatattg cgaccaccat atcggcgatc actcttcgat
     7081 gttggtttct tgtccaagag attagctttt tcaagatcat tgatagctct ctgaacagtc
     7141 cgtacagaaa cccccatacg tatggctaga ctttccattg acggatgcgg ccactcttgc
     7201 aaactccacc agtgaacgat caggttaagt agtgtgttaa aggccactga agttagcttt
     7261 ttctcgtttt gtataaaaaa caatacggta ggcactgctg tccagccaag agacaaaccg
     7321 ccagctttcc atttattctt aacggagtaa gtcattgatt ttcctaagcc ccaaaatatt
     7381 taaagtatat attatatggt atattcatat gaatagggtg acactggcgc cattattgtg
     7441 caaccaaaaa agactactct gaaaacgagg aaaagatttt ttcctgcctg aattagatac
     7501 ggagttagc
//
