LOCUS       sequence-388689-        9324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of PAL2, an AAV capsid with CNS 
            tropism in macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, 
            Solomon IH, Tabebordbar M, Sabeti PC
  TITLE     Systemic administration of novel engineered AAV capsids facilitates 
            enhanced transgene expression in the macaque CNS.
  JOURNAL   Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 
            10.1016/j.medj.2022.11.002.
  PUBMED    36417917
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Med (N Y). 
            2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002."
COMMENT     Sequence Label: kiCAP-AAV-PAL2
FEATURES             Location/Qualifiers
     source          1..9324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2784..2894
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2933..2951)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2934..2951)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2971..2988)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2977..3201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3263..3281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3300..3318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3337..3355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3374..3392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3411..3429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3448..3466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6183..6194
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6535..6555)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6535..6553)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6574..6590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6574..6590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6587..6609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6598..6614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6622..6628,6629..6646,6647..6652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6667..6688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6805..6822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6976..7564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7056..7075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7735..8544)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8458..8477
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8545..8649)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8675..9130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8812..8833)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9024..9043
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9257..9279
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9271..9288
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9272..9288
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9298..9317
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9298..9316
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtaccgggc cccccctcga tcgaggtcga cacgcgtgga ggggtggagt cgtgacgtga
       61 attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
      121 ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat
      181 tttgaagcgg gaggtttgaa cgcgcagccg ccatgccggg gttttacgag attgtgatta
      241 aggtccccag cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg
      301 tggccgagaa ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc
      361 aggcacccct gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg
      421 tgagtaaggc cccggaggct cttttctttg tgcaatttga gaagggagag agctacttcc
      481 acatgcacgt gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga
      541 gtcagattcg cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa
      601 actggttcgc ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg
      661 agtgctacat ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga
      721 ctaatatgga acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg
      781 cgcagcatct gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca
      841 attctgatgc gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt
      901 ggctcgtgga caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat
      961 acatctcctt caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg
     1021 cgggaaagat tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg
     1081 tggaggacat ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc
     1141 aatatgcggc ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca
     1201 tctggctgtt tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca
     1261 ctgtgccctt ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg
     1321 tcgacaagat ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg
     1381 ccaaagccat tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc
     1441 agatagaccc gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg
     1501 ggaactcaac gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac
     1561 tcacccgccg tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt
     1621 tccggtgggc aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg
     1681 gagccaagaa aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg
     1741 agtcagttgc gcagccatcg acgtcagacg cggaagcttc gatcaactac gcggacaggt
     1801 accaaaacaa atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat
     1861 gcgagagact gaatcagaat tcaaatatct gcttcactca cggtgtcaaa gactgtttag
     1921 agtgctttcc cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac
     1981 tgtgctacat tcatcacatc atgggaaagg tgccagacgc ttgcactgct tgcgacctgg
     2041 tcaatgtgga cttggatgac tgtgtttctg aacaataaat gacttaaacc aggtattgct
     2101 gccgatggtt atcttccaga ttggctcgag gacaacctta gtgaactagt gccaccatga
     2161 gtcggctgga taaatctaaa gtcataaacg gcgctctgga attactcaat gaagtcggta
     2221 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     2281 actggcacgt gaagaacaag cgggccctgc tcgatgccct ggccatcgag atgctggaca
     2341 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     2401 acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     2461 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     2521 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     2581 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     2641 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     2701 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     2761 agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag
     2821 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     2881 accttgacat gctccccggg taaatgcatg aattcgatct agagggccct attctatagt
     2941 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     3001 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3061 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3121 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3181 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgaatcaag
     3241 ctatcaagtg ccacctgacg tctccctatc agtgatagag aagtcgacac gtctcgagct
     3301 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg
     3361 acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag
     3421 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt accccctata
     3481 taagcagaga gatctgttca aatttgaact gactaagcgg ctcccgccag attttggcaa
     3541 gattactaag caggaagtca aggacttttt tgcttgggca aaggtcaatc aggtgccggt
     3601 gactcacgag tttaaagttc ccagggaatt ggcgggaact aaaggggcgg agaaatctct
     3661 aaaacgccca ctgggtgacg tcaccaatac tagctataaa agtctggaga agcgggccag
     3721 gctctcattt gttcccgaga cgcctcgcag ttcagacgtg actgttgatc ccgctcctct
     3781 gcgaccgcta gcttcgatca actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt
     3841 gggcatgaat ctgatgctgt ttccctgcag acaatgcgag agactgaatc agaattcaaa
     3901 tatctgcttc actcacggtg tcaaagactg tttagagtgc tttcccgtgt cagaatctca
     3961 acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc tacattcatc acatcatggg
     4021 aaaggtgcca gacgcttgca ctgcttgcga cctggtcaat gtggacttgg atgactgtgt
     4081 ttctgaacaa taaatgactt aaaccaggta tggctgccga tggttatctt ccagattggc
     4141 tcgaggacaa ccttagtgaa ggaattcgcg agtggtgggc tttgaaacct ggagcccctc
     4201 aacccaaggc aaatcaacaa catcaagaca acgctcgagg tcttgtgctt ccgggttaca
     4261 aataccttgg acccggcaac ggactcgaca agggggagcc ggtcaacgca gcagacgcgg
     4321 cggccctcga gcacgacaag gcctacgacc agcagctcaa ggccggagac aacccgtacc
     4381 tcaagtacaa ccacgccgac gccgagttcc aggagcggct caaagaagat acgtcttttg
     4441 ggggcaacct cgggcgagca gtcttccagg ccaaaaagag gcttcttgaa cctcttggtc
     4501 tggttgagga agcggctaag acggctcctg gaaagaagag gcctgtagag cagtctcctc
     4561 aggaaccgga ctcctccgcg ggtattggca aatcgggtgc acagcccgct aaaaagagac
     4621 tcaatttcgg tcagactggc gacacagagt cagtcccaga ccctcaacca atcggagaac
     4681 ctcccgcagc cccctcaggt gtgggatctc ttacaatggc ttcaggtggt ggcgcaccag
     4741 tggcagacaa taacgaaggt gccgatggag tgggtagttc ctcgggaaat tggcattgcg
     4801 attcccaatg gctgggggac agagtcatca ccaccagcac ccgaacctgg gccctgccca
     4861 cctacaacaa tcacctctac aagcaaatct ccaacagcac atctggagga tcttcaaatg
     4921 acaacgccta cttcggctac agcaccccct gggggtattt tgacttcaac agattccact
     4981 gccacttctc accacgtgac tggcagcgac tcatcaacaa caactgggga ttccggccta
     5041 agcgactcaa cttcaagctc ttcaacattc aggtcaaaga ggttacggac aacaatggag
     5101 tcaagaccat cgccaataac cttaccagca cggtccaggt cttcacggac tcagactatc
     5161 agctcccgta cgtgctcggg tcggctcacg agggctgcct cccgccgttc ccagcggacg
     5221 ttttcatgat tcctcagtac gggtatctga cgcttaatga tggaagccag gccgtgggtc
     5281 gttcgtcctt ttactgcctg gaatatttcc cgtcgcaaat gctaagaacg ggtaacaact
     5341 tccagttcag ctacgagttt gagaacgtac ctttccatag cagctacgct cacagccaaa
     5401 gcctggaccg actaatgaat ccactcatcg accaatactt gtactatctc tcaaagacta
     5461 ttaacggttc tggacagaat caacaaacgc taaaattcag tgtggccgga cccagcaaca
     5521 tggctgtcca gggaagaaac tacatacctg gacccagcta ccgacaacaa cgtgtctcaa
     5581 ccactgtgac tcaaaacaac aacagcgaat ttgcttggcc tggagcttct tcttgggctc
     5641 tcaatggacg taatagcttg atgaatcctg gacctgctat ggccagccac aaagaaggag
     5701 aggaccgttt ctttcctttg tctggatctt taatttttgg caaacaagga actggaagag
     5761 acaacgtgga tgcggacaaa gtcatgataa ccaacgaaga agaaattaaa actactaacc
     5821 cggtagcaac ggagtcctat ggacaagtgg ccacaaacca ccaggaggtt gggcctacgc
     5881 aaggcacagt tcgggcacag gcgcagaccg gctgggttca aaaccaagga atacttccgg
     5941 gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc aaaattcctc
     6001 acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg aagcacccgc
     6061 ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg gccttcaaca
     6121 aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc gtggagatcg
     6181 agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag tacacttcca
     6241 actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta tatagtgaac
     6301 cccgccccat tggcaccaga tacctgactc gtaatctgta attgcttgtt aatcaataaa
     6361 ccgtttaatt cgtttcagtt gaactttggt ctctgcgtat ttctttctta tctagtttcc
     6421 atggctacgt agataagtag catggcgggt taatcattaa ctacagatat ccatcacact
     6481 ggcggccgct cgactagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     6541 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6661 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6721 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6781 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa
     7741 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     7801 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     7861 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     7921 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     7981 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     8041 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     8101 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     8161 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     8221 taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg
     8281 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     8341 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     8401 tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc
     8461 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
     8521 cgtttcccgt tgaatatggc tcatactctt cctttttcaa tattattgaa gcatttatca
     8581 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8641 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     8701 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8761 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8821 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8881 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8941 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     9001 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     9061 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     9121 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     9181 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     9241 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     9301 tacgactcac tatagggcga attg
//
