LOCUS       sequence-388750-        8360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Honey bee gut symbiont broad-host range vector with constitutive 
            GFP, ampR and pTFC-FC2 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Chhun A, Moriano-Gutierrez S, Zoppi F, Cabirol A, Engel P, Schaerli 
            Y
  TITLE     An engineered bacterial symbiont allows noninvasive biosensing of 
            the honey bee gut environment.
  JOURNAL   PLoS Biol. 2024 Mar 5;22(3):e3002523. doi: 
            10.1371/journal.pbio.3002523. eCollection 2024 Mar.
  PUBMED    38442124
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2024-03-5"; volume: "22(3):e3002523. doi"; pages: " 
            10.1371/journal.pbio.3002523. eCollection 2024 Mar"
COMMENT     Sequence Label: pAC14
FEATURES             Location/Qualifiers
     source          1..8360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1120..1911,1912..1980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1743..1762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1981..2085)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(4361..4372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     RBS             4579..4587
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat taacccacag
       61 tacagacact gcgacaacgt ggcaattcgt cgcaatacaa cgctttggca gtttattctt
      121 gacatgtagt gagggggctg gtataatcac atagtactgt tatacagaaa cagaggagat
      181 attacatatg agtaaaggag aagagctttt cacaggagtt gtcccaatcc tcgtggaatt
      241 agacggtgat gttaatgggc acaagttctc tgtcagtgga gagggtgaag gcgacgcaac
      301 atatggcaag ctgaccctta aatttatttg caccacgggt aaactacctg ttccatggcc
      361 aacactggtc actacgttcg ggtatggggt tcagtgcttt gcgcgctacc cagatcacat
      421 gaaacagcac gactttttca agagtgcaat gcccgaaggc tatgtacagg agagaaccat
      481 cttttttaag gatgacggca actataagac acgcgccgaa gtgaagttcg agggtgatac
      541 ccttgttaat agaatcgagt taaagggtat tgactttaag gaagatggaa atattttagg
      601 ccacaaactg gaatataact ataactccca taatgtgtac attatggccg acaagcaaaa
      661 gaacggtatc aaggttaact tcaagatcag acacaacatt gaggatggaa gcgttcaact
      721 agccgaccat taccaacaaa acaccccaat tggcgatggg cctgtgctgt taccagacaa
      781 ccattacctg tccactcaat ctgccctttc gaaagatccc aacgaaaagc gcgaccacat
      841 ggtccttctt gagtttgtca cggctgctgg gattacacac ggcatggatg aactatacaa
      901 ataaatccgt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
      961 tggggggagt ttagggaaag agcatttgtc agctggaaat ctgctcgtca gtggcgagcg
     1021 gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1261 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2101 agtgccacct gtcatgacca aaatccctta acgtgctcaa ggcagccaga acatccaaaa
     2161 atccgctgta ggcgattctg atggcttctg ttcggggtat gaggcgatgt gctccacaag
     2221 cacgaccgac aagccagccg aaacgctcgc caagctggcc gctgcgctac ctgccggaga
     2281 ggccaaacgc taccgggtga tggtgtccat tgtcccggtg gaagaatgaa ggcgagcggg
     2341 gcggggcggg gtgtgtctgg ccgggcttgt cccggccttg tcttttgggg tttttggtaa
     2401 ggacttttga cgccctcaag ggcggcgaaa agtaccgcgc ctagtggatg atcttggata
     2461 gatcatggag ggattttgcg aatcgcccgc aaacccgcgc cgttgctagg tttttcagat
     2521 ttcggcgggg aacgttacgc cgggggttgg ggaacgttac gccgggggtt ggggaacgtt
     2581 acgccggggg ttgggcctgc tgatttccca cttgcccttg gcgtactcgt tcaccgtcca
     2641 accaacggcg gcaagctcga caagcgcctt gcgggcggtc tggcggcgct ttttcattgc
     2701 ttcatcgttg gctgcgtctg gccatacata accgcacagc gtgtcgattt cagctttgcc
     2761 agactttccg gggtcaatcc agccacatag ccgctggtgt agcagccgtg ccgggtctgt
     2821 ttccagcttg cgaacttctg ccatgttgat gcgggtgtgc gggcgctctc ccataaccgc
     2881 atccgccagc cggggattaa gcgcaacaaa taacttgcct tcttgctcgt cgctcgcgta
     2941 ctcggacaga atgcggaacc cttgccgctt accacctctt tcgacaatca ccgataccgc
     3001 ccaaagccgt tcgatgcttt cccggatggt tttaaactga cttcctccat cttcggcgta
     3061 gccaagttct cgggccaact ggcggaagct gcccttggct accatcgtat ctttctctat
     3121 ggcgtcccac cgcatatcaa gccataggcg gagttgctgg cctgcttctg attccgtttc
     3181 gtgccgtagc acgatgccgc gcccgttatc tccggaaatt gcagccattg ccaccagccc
     3241 ttgcaatacg cgcaagtcat cgccgccaag tggttcaggc ccccaaaaac ggattgagtc
     3301 atctccgtag ttgtaggtca catcgagctt gagcctcttt cgttctccgc gcttgagact
     3361 gcggaaaagc cccggcgtga ggcaatgcgc ggggtcgtgc cgcgcatggg tgaggtcata
     3421 gctcattgcg ctttccccct ttttttcctg ctccattgct gattgcctcc tgtagttcaa
     3481 cgggcaacaa caccccgccg ctgtgccgct gataccagcg gtctagcggg gtcgcgtcgt
     3541 agttctgctt gctcacgcca aagcggacaa aaaggccgcg ccgctcgttg ccgatgggcg
     3601 aacgatcaaa gccccggtca ctcatgcgct cggcttcctg ctccgtcatt ttggcgacat
     3661 agccgcacca tctggcgttg tcgatcaggg cagacgcgcc ccgcgctgcc tgctgctggt
     3721 cggtctggcc gtcgcgggcg ctgcccttgt tgacgtggtg caggtacagg acagccgccc
     3781 cggtggtcgc cgcgatgtgt tccaacacgg aaacaaggtg ggccatgtcg ccattgctgt
     3841 tctcgtcgag gctgtgaatc cggctcaggg tgtccagcac aatcagccgg gcccctgcgc
     3901 tgtagtcgat gacgcggcgc aagtgcgcct cgtccatcac gtttagccgc ttgcccatga
     3961 tcggctcaag catcaggttc tcagcgatgg cttcgcgggc cgactggttg aggtgctggc
     4021 cgatggcgtg gacacgtcgc acaagggcgg gctgtggatc ttcgccagcg agataaacca
     4081 cgcgcccggt gtgcgccggg gttagtccca caaggtcgcc gcctgccaca ctgcaagcga
     4141 ttgacatggc cgcttcaaga gcaaaaaagc tcttgccagt tgcgccaggt gcgacaagtg
     4201 cgcccacggt tccggccaag aatccgggcc agatgaaatc aagttctggc ggctcattgg
     4261 tgaaggccgc cataatgtct aaagccatgc ttgtctcctt ttcagacagt tgtggttagg
     4321 cgctgccggg gccgcaatcc cctgccgcgc cgctcaattt tcgcccctcg atgccagcca
     4381 cgccgaagcc tcggccaagg cgtcctcata ggcgtagctg gccgcttctt ctgcgttctc
     4441 tgctgtggcg tactcggaca acgggaacac tccgccgccg acttcctcgc cgctttccaa
     4501 caggcgcaac cgccagccgc cgccaaggtc ggaggggcgc gggtcgatct ggtagctgta
     4561 ttcgatcatt ggcggtaaac ctccttacga ttgccgatgc gcataacgag gatgcgcaaa
     4621 gcaccgtcct cgatgctggc gatgattcgc caatccccaa cgcggtattt ccagaaggct
     4681 cccagtttgg agcctttgag ggcttcgcca atgctgcgcg ggtcgtcgag ctgggcgaca
     4741 cggccatgca aaaaagcgag gatgcggcgg gcggtctgct ggtcgatctt gcctagctcg
     4801 cgctcggcgg ctgggtcgag ttcaaccttc catgccatag cgtttcatca cttcttcgag
     4861 tggcacggtt tgggttttgc ctgcgcgaat gtcgatcagg cgttgctctg caaggtacaa
     4921 atcttcgagg tcatccaagt gctcaaggat ggcttcgcgg gcatagaaag tcttggtccg
     4981 ccctgtggcc tgcgccagtg cttcaaggcg ggtttccact tcggcgggca gtcggattgc
     5041 aagcatgttt cggctcctgt tctgaaaacg ctatacaagt atatcacaac caccacgcaa
     5101 cgctagagac tcattcccgg cgagttgtcg cgctcgatct tctgcgcctt tgcctgctcc
     5161 tgctgtcgta caggctcgcg cccttccagc ttctcccact gctgccggta cttgccaaga
     5221 tcggcggctt gccggtcgcc tgcggcgctg taggcatagc gggcggtgcg ctgcgcgtag
     5281 tcgttccaat cgcggccctc gtctttctgc cgggtggccg gtgcgcactg gcggatagcg
     5341 ccctcgatgg ccgcttgatc gtggccggtg acgcgcatac gcacggcaat catggaatcc
     5401 acgcgggaca agtccacctc gccgccacgc tgccgcttga gcacgtcgcg gtaatgccgc
     5461 tggtaggcgt cgatcgcgct gccgctggcc gctgcaagct ccaaggcggg cttggctttg
     5521 ctgcgctcgg gctgctgcgc cttcaaggcc gcttgccgct gatactcggc gtcgatctgg
     5581 ctggacaagg ccagcgcctt gacgcactcc cggcgctcgg ccttgagcaa gcgcacctct
     5641 ggatagctgc cgtcctcccg ctggtgcttg ggcttgcggt tctcgtagcc gggtgcgcgg
     5701 tgcgggtgga tggctcccga tagcttcggg tcgccgtatt cacggttcag ggcatcgctc
     5761 aggcggttgc ccacgtcctt atcgtgggcc gtccccagct tcgacacggt gatgatggcc
     5821 tgatagttgc cggggctgga ttccagcacg gcggcgggct ggtagccgtc tttgataagc
     5881 cgctccagct tctcccggtt catgtcgtcg atgaggatgt gatgcttctt gtccgatagc
     5941 ggcgtgtagt agaggttttc gccccggcgc tgtaggcgct gcatctccgg cgtgcgctgc
     6001 tcgatctcct gcggtgtgaa accccgcgtg atgccgtcct tcttgtcgag gatgaaggtt
     6061 tgcttccttc cgtctgcctg catcttgatg gacgttaccc ggtagcgctc cgcgcctacc
     6121 gcttccgcgt accgctcgaa ctgctttagc tgctccgatt tcatcgcctg cgccctctcc
     6181 tgctgtatcc gctgccgctc ctgctggatg ctctcctgaa gctctgcatt tgtgatcttg
     6241 aagccgtgct ctgctgctaa cttcccgcac atggccttgt attcgtcgtt cccggttacg
     6301 gtgaagctgc cccacttctg cgccgatagc tgcaaggcgg caagcgtgct gtcccggttg
     6361 cgccagtcgt gaatatcgat gcttttgccc ttgtccacga aagacacacc cccgccccgc
     6421 cccccttctt ccttccggct gtaatgcacc tgctggccca cgatttcagg ctgataggcg
     6481 cgaatgtcgc gcggcgtcgg cggctcgctg cggtcgccct cgatgcgctg cggctcgctg
     6541 gcccggtgtc gccactgctc ggcaagctcc gggcttttct gcatccgctg ccactgctcc
     6601 aggtcgggat agggtctgaa ctgctgccgg tgctgctcgc gctgcttctg gtgcttctcc
     6661 ttcaaggcgg ctttctctgc ggcctgctcg gcggcaatca cgctgcgcat ggcgttgagc
     6721 acttcgccct tgcctttcca gttgccgcgc atgagttcat cgcggcgggc cttctgctgc
     6781 tcggcaagtt gcttgcgctc ctgctcctgc cgcttgtcct gtaccagctt gtccgcgttc
     6841 ttctccgagt agtgcgcctt gcgcccggtg atgtagtcct tccatcccgg cacgtctggc
     6901 ttgatcggct ccggctcgcg ctgtgctacc tgctgacgct gcggcggctg gtaagctccg
     6961 agtcgctttt gcaacttgga taggctcgcg tcccggtctg cgctgctggc cttcacgcca
     7021 acatcaccga caaagagcgt cgccccgctg cctgttttct cgtagcgcat ccccttggct
     7081 gctaactcgc ggtgcaactg ctcccacgtc tgcgcctttt tgatgatcgg tgcgccgtcc
     7141 tcaatggcga tgcgttcggc ggatttctcc ccggtgcggt tttccatgtc gcgcttcggc
     7201 tgggctggct ggcgcggctt gtccttgtcg atgtgctccc ggcctaactc accattctcc
     7261 aacacctgat aacgcccgtt ctgctcacgc tgccagcctt gtgcgttctc gattcgtgct
     7321 atcgccttgt gcgctgcctc aatgtcgaag ccgttattga tcttcaccac cttgagcgtt
     7381 tcaggatgca cgcggttgat ggcaaggtgc aggtgcaagt tgtccgtgtc ggcgtgcaaa
     7441 ccatagatgg cttgatggtc tttaacacct agctcatcca tgaaaatgct taccgcttct
     7501 tcgacctgct ccgggctggg ctgctcgcct tctcgccaac tcaggacata gtgattgatc
     7561 gtatccttgc ttcgcactgc ctcttgtgac aaggcaatca tttctgccgt ctggctctgt
     7621 ggttcatcgg tgatgaatcc acgcgccccg gcatggatgc acttctcctg cgagttctca
     7681 cgctcaggct cacggatgta gccagttagg cgggaaacgc gaaccgcctt gcttgctgcc
     7741 ttctgcgggt tcttaacctt cttgacgatc acggctcagt ttcccgatat aggctttcaa
     7801 gaccacaagc gccccggctg tgtccttgct gtacacgcct ccgctctcgt tgtggatgtg
     7861 cttaagcaat ccaccgatgc ggcgcaactc tttgagcatt accgcgtcgg cgctggcaat
     7921 gatcggcctg ccaaagtagc ggcggcggac aagctccgac atgctcaacc cggcaaggtc
     7981 ggcatcctct ttcaagcggg ccttctccgc tgcggtcagc cgtacattga taacggcgtc
     8041 cagcggttcc ggcccctgcg tttcaaatgg catcgtgtct ccttctcgcg gggggcgggc
     8101 ggggtgtgtc ttggacagta agaagcgaga gcgccgcact gtccggggtc aaggggatac
     8161 cccttgcagg ataagccaga ccgtgagaag cgaagcgccg aatgggcagg cgtccgttgg
     8221 aaccacgtgg cgaagccatg tgtgttacaa cggtcatcct gtattgctca accgctctac
     8281 tatcatatca gcagtttagg cacactagat gcactttgca acaagcataa aaggcgctat
     8341 gctgtcaact ccgaaaccag
//
