LOCUS       sequence-388777-        7984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Serotonin transporter with EYFP fluorescent protein driven by 
            CaMKIIa promoter on a AAV vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Vargas MV, Dunlap LE, Dong C, Carter SJ, Tombari RJ, Jami SA, 
            Cameron LP, Patel SD, Hennessey JJ, Saeger HN, McCorvy JD, Gray JA, 
            Tian L, Olson DE
  TITLE     Psychedelics promote neuroplasticity through the activation of 
            intracellular 5-HT2A receptors.
  JOURNAL   Science. 2023 Feb 17;379(6633):700-706. doi: 
            10.1126/science.adf0435. Epub 2023 Feb 16.
  PUBMED    36795823
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf0435"; journalName: "Science"; date: 
            "2023-02-17- 17"; volume: "379"; issue: "6633"; pages: "700-706"
COMMENT     Sequence Label: pAAV_CaMKIIa_SERT_EYFP
FEATURES             Location/Qualifiers
     source          1..7984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..46)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     237..258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(412..431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     531..553
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(591..609)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        677..781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(782..850,851..1642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1000..1019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1813..2401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2302..2321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2463..2592
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2611..3899
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3924..3933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5832..5841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5838..5840,5841..5843,5844..6557)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5883..5904)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6144..6163)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6491..6512
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6573..7161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6626..6646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7044..7055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7193..7669
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(7392..7411)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   7709..7849
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7709..7838
                     /label=AAV2 ITR
     rep_origin      join(7924..7984,1..395)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
       61 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      121 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
      181 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      241 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
      301 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      361 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
      421 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
      481 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      541 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
      601 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      781 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      841 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      901 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      961 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1021 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1081 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1141 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1201 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1261 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1321 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1381 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1441 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1501 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1561 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1621 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1681 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1921 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2461 gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct
     2521 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
     2581 ctaggggttc ctgcggccgc acgcgtttaa cattatggcc ttaggtcact tcatctccat
     2641 ggggttcttc ttctgatttt ctagaaaatg agatgggggt gcagagagct tcctcagtga
     2701 cctgcccagg gtcacatcag aaatgtcaga gctagaactt gaactcagat tactaatctt
     2761 aaattccatg ccttgggggc atgcaagtac gatatacaga aggagtgaac tcattagggc
     2821 agatgaccaa tgagtttagg aaagaagagt ccagggcagg gtacatctac accacccgcc
     2881 cagccctggg tgagtccagc cacgttcacc tcattatagt tgcctctctc cagtcctacc
     2941 ttgacgggaa gcacaagcag aaactgggac aggagcccca ggagaccaaa tcttcatggt
     3001 ccctctggga ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg ccctgctgct
     3061 cagtggtgac agataggggt gagaaagcag acagagtcat tccgtcagca ttctgggtct
     3121 gtttggtact tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc taaaaagcag
     3181 ggagagctgg aaagaaacaa ggacagagac agaggccaag tcaaccagac caattcccag
     3241 aggaagcaaa gaaaccatta cagagactac aagggggaag ggaaggagag atgaattagc
     3301 ttcccctgta aaccttagaa cccagctgtt gccagggcaa cggggcaata cctgtctctt
     3361 cagaggagat gaagttgcca gggtaactac atcctgtctt tctcaaggac catcccagaa
     3421 tgtggcaccc actagccgtt accatagcaa ctgcctcttt gccccactta atcccatccc
     3481 gtctgttaaa agggccctat agttggaggt gggggaggta ggaagagcga tgatcacttg
     3541 tggactaagt ttgttcgcat ccccttctcc aaccccctca gtacatcacc ctgggggaac
     3601 agggtccact tgctcctggg cccacacagt cctgcagtat tgtgtatata aggccagggc
     3661 aaagaggagc aggttttaaa gtgaaaggca ggcaggtgtt ggggaggcag ttaccggggc
     3721 aacgggaaca gggcgtttcg gaggtggttg ccatggggac ctggatgctg acgaaggctc
     3781 gcgaggctgt gagcagccac agtgccctgc tcagaagccc caagctcgtc agtcaagccg
     3841 gttctccgtt tgcactcagg agcacgggca ggcgagtggc ccctagttct gggggcagct
     3901 ctagagcggt accggatcca cccgccacca tggagacgac gcccttgaat tctcagaagc
     3961 agctatcagc gtgtgaagat ggagaagatt gtcaggaaaa cggagttcta cagaaggttg
     4021 ttcccacccc aggggacaaa gtggagtccg ggcaaatatc caatgggtac tcagcagttc
     4081 caagtcctgg tgcgggagat gacacacggc actctatccc agcgaccacc accaccctag
     4141 tggctgagct tcatcaaggg gaacgggaga cctggggcaa gaaggtggat ttccttctct
     4201 cagtgattgg ctatgctgtg gacctgggca atgtctggcg cttcccctac atatgttacc
     4261 agaatggagg gggggcattc ctcctcccct acaccatcat ggccattttt gggggaatcc
     4321 cgctctttta catggagctc gcactgggac agtaccaccg aaatggatgc atttcaatat
     4381 ggaggaaaat ctgcccgatt ttcaaaggga ttggttatgc catctgcatc attgcctttt
     4441 acattgcttc ctactacaac accatcatgg cctgggcgct atactacctc atctcctcct
     4501 tcacggacca gctgccctgg accagctgca agaactcctg gaacactggc aactgcacca
     4561 attacttctc cgaggacaac atcacctgga ccctccattc cacgtcccct gctgaagaat
     4621 tttacacgcg ccacgtcctg cagatccacc ggtctaaggg gctccaggac ctggggggca
     4681 tcagctggca gctggccctc tgcatcatgc tgatcttcac tgttatctac ttcagcatct
     4741 ggaaaggcgt caagacctct ggcaaggtgg tgtgggtgac agccaccttc ccttatatca
     4801 tcctttctgt cctgctggtg aggggtgcca ccctccctgg agcctggagg ggtgttctct
     4861 tctacttgaa acccaattgg cagaaactcc tggagacagg ggtgtggata gatgcagccg
     4921 ctcagatctt cttctctctt ggtccgggct ttggggtcct gctggctttt gctagctaca
     4981 acaagttcaa caacaactgc taccaagatg ccctggtgac cagcgtggtg aactgcatga
     5041 cgagcttcgt ttcgggattt gtcatcttca cagtgctcgg ttacatggct gagatgagga
     5101 atgaagatgt gtctgaggtg gccaaagacg caggtcccag cctcctcttc atcacgtatg
     5161 cagaagcgat agccaacatg ccagcgtcca ctttctttgc catcatcttc tttctgatgt
     5221 taatcacgct gggcttggac agcacgtttg caggcttgga gggggtgatc acggctgtgc
     5281 tggatgagtt cccacacgtc tgggccaagc gccgggagcg gttcgtgctc gccgtggtca
     5341 tcacctgctt ctttggttct ctggtcaccc tgacttttgg aggggcctac gtggtgaagc
     5401 tgctggagga gtatgccacg gggcccgcag tgctcactgt cgcgctgatc gaagcagtcg
     5461 ctgtgtcttg gttctatggc atcactcagt tctgcaggga cgtgaaggaa atgctcggct
     5521 tcagcccggg gtggttctgg aggatctgct gggtggccat cagccctctg tttctcctgt
     5581 tcatcatttg cagttttctg atgagcccgc cacaactacg acttttccaa tataattatc
     5641 cttactggag tatcatcttg ggttactgca taggaacctc atctttcatt tgcatcccca
     5701 catatatagc ttatcggttg atcatcactc cagggacatt taaagagcgt attattaaaa
     5761 gtattacccc agaaacacca acagaaattc cttgtgggga catccgcttg aatgctgtgg
     5821 gaattccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5881 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     5941 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6001 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct
     6061 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6121 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6181 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6241 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6301 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     6361 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6421 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     6481 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     6541 acgagctgta caagtaaagc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     6601 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6661 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6721 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6781 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6841 tcctttccgg aactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6901 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6961 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
     7021 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7081 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7141 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     7201 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     7261 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     7321 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     7381 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     7441 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     7501 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     7561 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     7621 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     7681 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     7741 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     7801 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     7861 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     7921 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7981 ccgc
//
