LOCUS       sequence-388780-        7980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Serotonin transporter with mCherry fluorescent protein driven by 
            CaMKIIa promoter on a AAV vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Vargas MV, Dunlap LE, Dong C, Carter SJ, Tombari RJ, Jami SA, 
            Cameron LP, Patel SD, Hennessey JJ, Saeger HN, McCorvy JD, Gray JA, 
            Tian L, Olson DE
  TITLE     Psychedelics promote neuroplasticity through the activation of 
            intracellular 5-HT2A receptors.
  JOURNAL   Science. 2023 Feb 17;379(6633):700-706. doi: 
            10.1126/science.adf0435. Epub 2023 Feb 16.
  PUBMED    36795823
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf0435"; journalName: "Science"; date: 
            "2023-02-17- 17"; volume: "379"; issue: "6633"; pages: "700-706"
COMMENT     Sequence Label: pAAV_CaMKIIa_SERT_mCherry
FEATURES             Location/Qualifiers
     source          1..7980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1243..1252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3151..3160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3157..3867
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3302..3320)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3570..3589
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3892..4480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3945..3965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4363..4374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4512..4988
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4711..4730)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5028..5168
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5028..5157
                     /label=AAV2 ITR
     rep_origin      5243..5698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5330..5349)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5540..5561
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5715..5734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5834..5856
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5894..5912)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5980..6084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6085..6153,6154..6945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6303..6322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7116..7704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7605..7624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   7766..7895
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        join(7914..7980,1..1222)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
ORIGIN
        1 ggtgcagaga gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa
       61 cttgaactca gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac
      121 agaaggagtg aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc
      181 agggtacatc tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat
      241 agttgcctct ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc
      301 ccaggagacc aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag
      361 gcctcaggag gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt
      421 cattccgtca gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga
      481 tatgcacaat ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc
      541 aagtcaacca gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg
      601 aagggaagga gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg
      661 caacggggca atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt
      721 ctttctcaag gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc
      781 tttgccccac ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag
      841 gtaggaagag cgatgatcac ttgtggacta agtttgttcg catccccttc tccaaccccc
      901 tcagtacatc accctggggg aacagggtcc acttgctcct gggcccacac agtcctgcag
      961 tattgtgtat ataaggccag ggcaaagagg agcaggtttt aaagtgaaag gcaggcaggt
     1021 gttggggagg cagttaccgg ggcaacggga acagggcgtt tcggaggtgg ttgccatggg
     1081 gacctggatg ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag
     1141 ccccaagctc gtcagtcaag ccggttctcc gtttgcactc aggagcacgg gcaggcgagt
     1201 ggcccctagt tctgggggca gctctagagc ggtaccccac ccgccaccat ggagacgacg
     1261 cccttgaatt ctcagaagca gctatcagcg tgtgaagatg gagaagattg tcaggaaaac
     1321 ggagttctac agaaggttgt tcccacccca ggggacaaag tggagtccgg gcaaatatcc
     1381 aatgggtact cagcagttcc aagtcctggt gcgggagatg acacacggca ctctatccca
     1441 gcgaccacca ccaccctagt ggctgagctt catcaagggg aacgggagac ctggggcaag
     1501 aaggtggatt tccttctctc agtgattggc tatgctgtgg acctgggcaa tgtctggcgc
     1561 ttcccctaca tatgttacca gaatggaggg ggggcattcc tcctccccta caccatcatg
     1621 gccatttttg ggggaatccc gctcttttac atggagctcg cactgggaca gtaccaccga
     1681 aatggatgca tttcaatatg gaggaaaatc tgcccgattt tcaaagggat tggttatgcc
     1741 atctgcatca ttgcctttta cattgcttcc tactacaaca ccatcatggc ctgggcgcta
     1801 tactacctca tctcctcctt cacggaccag ctgccctgga ccagctgcaa gaactcctgg
     1861 aacactggca actgcaccaa ttacttctcc gaggacaaca tcacctggac cctccattcc
     1921 acgtcccctg ctgaagaatt ttacacgcgc cacgtcctgc agatccaccg gtctaagggg
     1981 ctccaggacc tggggggcat cagctggcag ctggccctct gcatcatgct gatcttcact
     2041 gttatctact tcagcatctg gaaaggcgtc aagacctctg gcaaggtggt gtgggtgaca
     2101 gccaccttcc cttatatcat cctttctgtc ctgctggtga ggggtgccac cctccctgga
     2161 gcctggaggg gtgttctctt ctacttgaaa cccaattggc agaaactcct ggagacaggg
     2221 gtgtggatag atgcagccgc tcagatcttc ttctctcttg gtccgggctt tggggtcctg
     2281 ctggcttttg ctagctacaa caagttcaac aacaactgct accaagatgc cctggtgacc
     2341 agcgtggtga actgcatgac gagcttcgtt tcgggatttg tcatcttcac agtgctcggt
     2401 tacatggctg agatgaggaa tgaagatgtg tctgaggtgg ccaaagacgc aggtcccagc
     2461 ctcctcttca tcacgtatgc agaagcgata gccaacatgc cagcgtccac tttctttgcc
     2521 atcatcttct ttctgatgtt aatcacgctg ggcttggaca gcacgtttgc aggcttggag
     2581 ggggtgatca cggctgtgct ggatgagttc ccacacgtct gggccaagcg ccgggagcgg
     2641 ttcgtgctcg ccgtggtcat cacctgcttc tttggttctc tggtcaccct gacttttgga
     2701 ggggcctacg tggtgaagct gctggaggag tatgccacgg ggcccgcagt gctcactgtc
     2761 gcgctgatcg aagcagtcgc tgtgtcttgg ttctatggca tcactcagtt ctgcagggac
     2821 gtgaaggaaa tgctcggctt cagcccgggg tggttctgga ggatctgctg ggtggccatc
     2881 agccctctgt ttctcctgtt catcatttgc agttttctga tgagcccgcc acaactacga
     2941 cttttccaat ataattatcc ttactggagt atcatcttgg gttactgcat aggaacctca
     3001 tctttcattt gcatccccac atatatagct tatcggttga tcatcactcc agggacattt
     3061 aaagagcgta ttattaaaag tattacccca gaaacaccaa cagaaattcc ttgtggggac
     3121 atccgcttga atgctgtggg aattggatcc gccaccatgg tgagcaaggg cgaggaggat
     3181 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     3241 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3301 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3361 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3421 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3481 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     3541 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     3601 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     3661 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     3721 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     3781 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     3841 ggcggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcga taatcaacct
     3901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttgggga
     4141 attgccacca cctgtcagct cctttccgga actttcgctt tccccctccc tattgccacg
     4201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4261 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt
     4321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4441 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc
     4501 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     4561 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     4621 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     4681 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     4741 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     4801 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     4861 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     4921 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     4981 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     5041 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     5101 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     5161 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5221 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5281 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     5341 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5401 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     5461 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5521 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     5581 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     5641 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     5701 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5761 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5821 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5881 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5941 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6781 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6961 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7021 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7081 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7141 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7201 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7261 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7321 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7381 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7441 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7501 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7561 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7621 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7681 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7741 ttttgctggc cttttgctca catgtcctgc aggcagctgc gcgctcgctc gctcactgag
     7801 gccgcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag
     7861 agagggagtg gccaactcca tcactagggg ttcctgcggc cgcacgcgtt taacattatg
     7921 gccttaggtc acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg
//
