LOCUS       sequence-388781-        6079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses psychLight1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Dong C, Ly C, Dunlap LE, Vargas MV, Sun J, Hwang IW, Azinfar A, Oh 
            WC, Wetsel WC, Olson DE, Tian L
  TITLE     Psychedelic-inspired drug discovery using an engineered biosensor.
  JOURNAL   Cell. 2021 Apr 8. pii: S0092-8674(21)00374-3. doi: 
            10.1016/j.cell.2021.03.043.
  PUBMED    33915107
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 8. pii: S0092-8674(21)00374-3. doi: 10.1016/j.cell.2021.03.043."
COMMENT     Sequence Label: pCMV_psychLight1
FEATURES             Location/Qualifiers
     source          1..6079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        44..247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     198..218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1405..1426
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1532..1553)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1793..1812)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2546..2667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2583..2602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2637..2656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2674..3129)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2811..2832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3023..3042
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3156..3260
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3262..3619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3262..3453
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3285..3305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3470..3605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3654..4448
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3654..4448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3708..3727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4318..4337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4636..4655)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4680..4727
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5056..5644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5545..5564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(5819..6079,1..43)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
       61 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      121 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      181 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      241 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc
      301 aagcttaccg gtgcgcgcca tgaagacgat catcgccctg agctacatct tctgcctggt
      361 gttcgccgac tacaaggacg atgatgacgc catggacata ctttgtgaag agaatacttc
      421 actctcttct actactaact ctcttatgca actgaacgat gatacccgat tgtactcaaa
      481 cgacttcaat tccggcgaag cgaacaccag tgacgcattc aactggactg tcgattctga
      541 aaacagaact aatctgtcat gcgagggttg tcttagtccc tcttgtctca gcctgttgca
      601 cctccaggaa aagaactggt cagcactgct cactgcggta gtgataatac tcactattgc
      661 tggcaatatt ctcgtaatta tggcagtctc cttggagaag aaactccaaa acgccacaaa
      721 ttattttctt atgagccttg ccatcgcaga tatgctcttg ggatttttgg tgatgcctgt
      781 gagtatgctc acgatactgt atggatatcg ctggcctctg ccgtctaaac tttgcgctgt
      841 gtggatttac ttggatgtcc ttttttcaac tgcgagtatt atgcatcttt gcgccattag
      901 tcttgatagg tatgtggcta tccaaaatcc tgctcaccat tcccgcttta atagtagaac
      961 taaggctttt ctgaaaataa tagcagtgtg gaccatatct gtcggcataa gcatgcctat
     1021 ccccgtattt ggacttcaag atgactcaaa ggtattcaaa gaagggtcat gtctgctggc
     1081 cgatgacaat ttcgtgctta ttggatcctt cgtcagtttc ttcattcctt tgacaatcat
     1141 ggtgattacc tactttctta cgattaaatc tttgcaaaag cagctgagct caggttataa
     1201 cgtctatatc aaggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca
     1261 caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg
     1321 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa
     1381 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1441 cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg
     1501 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1561 ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct
     1621 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1681 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1741 caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg
     1801 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1861 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1921 ctttaatatg catgaccaac tgaatgagca aaaggcttgt aaggtactcg gcatagtctt
     1981 ctttctgttt gtggtgatgt ggtgtccctt ctttataacg aatatcatgg cagtgatctg
     2041 caaggaatca tgcaatgagg atgtgatcgg ggcacttctg aacgttttcg tgtggatagg
     2101 gtatctgtca agtgctgtga acccactggt ctataccttg tttaataaga cataccgctc
     2161 agccttttca cggtatattc aatgtcagta taaggaaaac aagaaacctc tgcaacttat
     2221 tcttgtgaac actatccctg ccctggctta taagtcatca cagttgcaga tgggccagaa
     2281 aaaaaattcc aagcaggacg cgaagacaac agacaacgat tgtagtatgg ttgccctcgg
     2341 caagcagcac agtgaagaag cgagcaaaga caatagtgat ggcgtaaacg aaaaagtcag
     2401 ttgtgtataa caacctgatg tacagcggcc gcgactctag atcataatca gccataccac
     2461 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2521 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2641 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     2701 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     2761 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     2821 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     2881 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2941 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3001 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3061 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3121 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3181 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3241 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3301 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3361 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3421 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3481 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3601 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     3661 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3721 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3781 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     3841 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3901 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3961 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4021 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4081 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4141 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4201 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4261 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4321 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4381 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4441 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4501 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4561 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4621 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     4681 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     4741 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     4801 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     4861 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     4921 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4981 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5041 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5101 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5161 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5221 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5281 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5341 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5401 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5461 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5521 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5581 tcgccacctc tgacttgagc gtcgattatt gtgatgctcg tcaggggggc ggagcctatg
     5641 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5701 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
     5761 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5821 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5881 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5941 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     6001 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     6061 tgaccttatg ggactttcc
//
