LOCUS       sequence-388788-        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eukaryotic expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  TITLE     Eukaryotic expression plasmid with a puromycin selection marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-(-)-Pur
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(164..181)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    170..394
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      440..868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(527..546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     737..758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(877..897)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        882..1210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1062..1196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1123..1142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ttgaataata cgactcacta tagggagacc caagctggct agcgtttaaa cgggccctct
       61 agactcgagc ggccgccact gtgctggata tctgcagaat tccaccacac tggactagtg
      121 gatccgagct cggtaccaag cttaagttta aaccgctgat cagcctcgac tgtgccttct
      181 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      241 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      301 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
      361 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
      421 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      481 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      541 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      601 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      661 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      721 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      781 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      841 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
      901 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      961 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     1021 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     1081 ccagttccgc ccattctccg ccccatggct gactaatttt tttatttatg cagaggccga
     1141 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1201 cttttgcaaa agctcccggg agctgg
//
