LOCUS       sequence-388789-        1247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eukaryotic expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1247)
  TITLE     Eukaryotic expression plasmid with a puromycin selection marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-(-)-Pur
FEATURES             Location/Qualifiers
     source          1..1247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(135..153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(198..401)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(227..247)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        402..781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     953..972
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1047..1151
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaagggtta acttaagctt gggtaccgag ctcggatcca ctagtccagt gtggtggaat
       61 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacgctag
      121 ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      181 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      241 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      301 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      361 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      421 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      481 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
      541 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
      601 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
      661 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
      721 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
      781 caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
      841 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
      901 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca
      961 gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt
     1021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaag
//
