LOCUS       sequence-388791-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eukaryotic expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  TITLE     Eukaryotic expression plasmid with a puromycin selection marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-(-)-Pur
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(634..963)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      648..783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     948..968
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atcaccaggg tcctaggtcc ctcgggcacc tcgacatcgg cggtcacggt gaagcccagg
       61 cgctcgtaga atggaaggtt tcttggtgcg cttgtttcga gaaaggcggg cacaccggcg
      121 cgttcggcgg cctctacgcc gggcaacacg acggcgctgc ccaagccctt gccctggtgg
      181 tcggggctca ctcctacagt ggccagaaac caggcaggct ccttgggacg atggggggca
      241 agcaggccct ccatttgctg ctgggcggcc aggcggctgc cgctcagctc ggccattcgg
      301 gggccgatct cggcgaacac ggcgccggcc tcaacgctct cgggggtagt ccacacggcc
      361 acggccgcgc cgtcgtcggc tacccacacc ttgccgatgt ccagcccgac gcgggtcagg
      421 aacagctcct gcaactctgt cacacgctcg atgtgccggt cggggtccac ggtgtgccgg
      481 gtagcggggt agtcggcgaa agcggcggcc agagtgcgga cggcgcgggg cacatcatcg
      541 cgagtggcca ggcgcacggt aggcttgtac tcggtcatcc tcaggcacgt gctgatcaga
      601 tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa
      661 gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcagcct ctgcataaat
      721 aagaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
      781 gatgggcgga gttaggagcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      841 acttctgcct gctggggagc ctggggactt tccacacctg ggtgctgact aattgagatg
      901 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      961 cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttgtaaccaa
     1021 taggcccaaa tcggcaaaat cccttataaa tccaaa
//
