LOCUS       sequence-388794-        1219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1219)
  TITLE     Eukaryotic expression plasmid with a puromycin selection marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-Pur-mEGFP
FEATURES             Location/Qualifiers
     source          1..1219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(72..779,780..782,783..785))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLKE
                     RVTAAGITLGMDELYK"
     primer_bind     463..482
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     722..743
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(830..849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(831..849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(894..1097)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(923..943)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaagggttaa cttaagcttg ggtaccgagc tcggatccac tagtccagtg tggtggaatt
       61 ctgcagatat ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgcgc
      121 tccttcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg
      181 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      241 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      301 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      361 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      421 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      481 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      541 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
      601 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      661 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      721 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc gcccttgctc
      781 accatctcga gtctagaggg cccgtttaaa cgctagccag cttgggtctc cctatagtga
      841 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      901 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      961 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     1021 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     1081 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     1141 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     1201 acgtcaatag ggggcgtaa
//
