LOCUS       sequence-38886-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Gunewardene MS, Subach FV, Gould TJ, Penoncello GP, Gudheti MV, 
            Verkhusha VV, Hess ST
  TITLE     Superresolution imaging of multiple fluorescent proteins with highly
            overlapping emission spectra in living cells.
  JOURNAL   Biophys J. 2011 Sep 21;101(6):1522-8. Epub 2011 Sep 20.
  PUBMED    21943434
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2011-09-21"; volume: "101(6)"; pages: "1522-8. Epub 2011
            Sep 20"
COMMENT     Sequence Label: pBAD/HisB-PAmKate
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgagctc gagatctatg
      181 agcgagctga tcaccgagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
      241 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
      301 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
      361 ttcatgtacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttctggaag
      421 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
      481 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
      541 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
      601 gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcaga
//
