LOCUS       sequence-388869-        5804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NIR-GECO1 preferentially in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Qian Y, Piatkevich KD, Mc Larney B, Abdelfattah AS, Mehta S, Murdock
            MH, Gottschalk S, Molina RS, Zhang W, Chen Y, Wu J, Drobizhev M, 
            Hughes TE, Zhang J, Schreiter ER, Shoham S, Razansky D, Boyden ES, 
            Campbell RE
  TITLE     A genetically encoded near-infrared fluorescent calcium ion 
            indicator.
  JOURNAL   Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6.
            Epub 2019 Jan 21.
  PUBMED    30664778
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0294-6"; journalName: "Nat Methods"; date: 
            "2019-02"; volume: "16"; issue: "2"; pages: "171-174"
COMMENT     Sequence Label: pAAV-hSyn-NES-NIR-GECO1
FEATURES             Location/Qualifiers
     source          1..5804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    2031..2619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2084..2104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2502..2513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2622..2638)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2623..2639)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2668..2789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2679..2698)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2733..2752
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2951..3080
                     /label=AAV2 ITR
     rep_origin      complement(3317..3905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3397..3416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4076..4867,4868..4936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4699..4718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4937..5041)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5067..5522)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5204..5225)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5416..5435
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5594..5723
                     /label=AAV2 ITR
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt caacaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gaattcgggg atccatgctg cagaacgagc
      481 ttgctcttaa gttggctgga cttgatatta acaagactgg aggaggttct atgtcggtac
      541 cgctgactac ctcagcattc ggccacgcgt ttctggctaa ctgtgaacgt gagcagatcc
      601 acctggcggg ctccattcag ccgcacggta tcctgctggc tgttaaagag cctgacaacg
      661 tggtgatcca ggcttctatt aacgctgcgg agttcctgaa caccaacttt gttgttggcc
      721 gtccgctgcg tgacctgggc ggcgatctgc ctttgcagat cctgccgcac ctgaacggcc
      781 cgctgcacct ggctccgatg accctgcgtt gtactgtggg ttctccgccg cgtcgtgtgg
      841 actgtaccat tcatcgaccg tctaacggcg gcctgatcgt agaactggaa ccagcaacca
      901 aggccactaa cattgcgccg gctctggtcg gtgcgcttca tcgtatcact tcttcatcct
      961 ccctgatggg cctgtgtgac gaaaccgcga ctattttccg tgagattact ggcttcgacc
     1021 gtgtgatggt aatgcgtctc ggcgcgcttg acgatctgac tgaagagcag atcgcagaga
     1081 ttaaagaggc tttctcccta tttgacaagg acggggacgg gacgataaca accaaggagc
     1141 tggggacggt gttccggtct ctggggcaga accccacaga agcagagctg caggacatga
     1201 tcaatgaagt agatgccgat ggcgacggca cattcgactt ccctgagttc ctgacgatga
     1261 tggcaaggaa aatgaatgac tcagacagtg aagaggaaat tagagaagcg ttccgcgtgt
     1321 ttgataagga cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc
     1381 ttggtgagaa gttaactgat gaggaggttg atgaaatgat cagggtagca gacaacgatg
     1441 gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag ggtggcggag
     1501 gttctgtaga ttcatcacgt cgtaagtgga ataaggcagg tcacgcagtc agagctatag
     1561 gtcggctgag ctcgcgtagg catgatttgc tgtccgaatg tcgtcgtgcg gacctggaag
     1621 cgttcctggg taaccgctac ccggcgtcta ctattccgca gatcgctcgt cgcctgtacg
     1681 aacttaaccg tgttcgcctg ctggtagatg tgaactatac tccggttccg ctacagccgc
     1741 gcatcagccc gctgaacggt cgtgatctgg atatgtccct gtcttgcctg cgctctatgt
     1801 ccccgatcca ccagaaatac atgcaggaca tgggcgttgg cgcaaccctg gtttgctctc
     1861 tgatggtgtc tggtcgtctg tggggtctga tcgtttgcca ccactacgaa ccgcgcttcg
     1921 ttccgtccca cattcgcgct gctggcgaag cgctggcgga aacttgtgcg aaccgcatcg
     1981 cgacgctgga gagctttgca cagtctcagt ccaaatgaaa gcttatcgat aatcaacctc
     2041 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2101 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2161 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2221 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2281 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2341 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2401 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2461 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2521 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2581 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac
     2641 ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     2701 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2761 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2821 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     2881 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     2941 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3001 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3061 gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5041 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     5101 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     5161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5281 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5341 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5401 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5461 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5521 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     5581 gctattacgc cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg
     5641 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     5701 caactccatc actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac
     5761 gtagccatgc tctaggaaga tctctgcaga gggccctgcg tatg
//
