LOCUS       sequence-388876-       10701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-2 
            alpha-C fusion protein in yeast (yeast two-hybrid or yeast 
            three-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Mattera R, Boehm M, Chaudhuri R, Prabhu Y, Bonifacino JS
  TITLE     Conservation and diversification of dileucine signal recognition by 
            adaptor protein (AP) complex variants.
  JOURNAL   J Biol Chem. 2011 Jan 21;286(3):2022-30. doi: 
            10.1074/jbc.M110.197178. Epub 2010 Nov 19.
  PUBMED    21097499
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.197178"; journalName: "J Biol Chem"; date: 
            "2011-01-21- 21"; volume: "286"; issue: "3"; pages: "2022-30"
COMMENT     Sequence Label: pGADT7-alpha-C
FEATURES             Location/Qualifiers
     source          1..10701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(61..78)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(232..820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(312..331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(991..1782,1783..1851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1614..1633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1852..1956)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2024..2042
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2080..2102)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2202..2221
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      2238..3402
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        4176..4880
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4926..4946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4962..5303
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     5309..5328
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5309..5327
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5334..5343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5346..5372
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      8645..8832
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8700..8724)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8949..10043)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(10044..10449)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(10491..10507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10491..10507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10504..10526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10515..10531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10539..10545,10546..10563,10564..10569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10584..10605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    10660..10693
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
       61 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      961 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1021 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1081 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1141 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1201 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1261 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1321 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1381 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1441 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1501 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1561 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1621 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1681 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1741 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1801 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1861 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1921 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     1981 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2041 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     2101 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     2161 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     2221 actgagagtg caccataacg catttaagca taaacacgca ctatgccgtt cttctcatgt
     2281 atatatatat acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc
     2341 gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc
     2401 cgaagttcct attctctagc tagaaagtat aggaacttca gagcgctttt gaaaaccaaa
     2461 agcgctctga agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta
     2521 aaatattgcg aataccgctt ccacaaacat tgctcaaaag tatctctttg ctatatatct
     2581 ctgtgctata tccctatata acctacccat ccacctttcg ctccttgaac ttgcatctaa
     2641 actcgacctc tacatttttt atgtttatct ctagtattac tctttagaca aaaaaattgt
     2701 agtaagaact attcatagag tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac
     2761 gtagtatata gagacaaaat agaagaaacc gttcataatt ttctgaccaa tgaagaatca
     2821 tcaacgctat cactttctgt tcacaaagta tgcgcaatcc acatcggtat agaatataat
     2881 cggggatgcc tttatcttga aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg
     2941 ggaagtggag tcaggctttt tttatggaag agaaaataga caccaaagta gccttcttct
     3001 aaccttaacg gacctacagt gcaaaaagtt atcaagagac tgcattatag agcgcacaaa
     3061 ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata gcgctctcgg gatgcatttt
     3121 tgtagaacaa aaaagaagta tagattcttt gttggtaaaa tagcgctctc gcgttgcatt
     3181 tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc
     3241 atttttgttt tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt
     3301 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     3361 gttgcatttt tgttctacaa aatgaagcac agatgcttcg ttgcttgcat gcctgcaggt
     3421 cgagatccgg gatcgaagaa atgatggtaa atgaaatagg aaatcaagga gcatgaaggc
     3481 aaaagacaaa tataagggtc gaacgaaaaa taaagtgaaa agtgttgata tgatgtattt
     3541 ggctttgcgg cgccgaaaaa acgagtttac gcaattgcac aatcatgctg actctgtggc
     3601 ggacccgcgc tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc ccggcggagt
     3661 tttttgcgcc tgcattttcc aaggtttacc ctgcgctaag gggcgagatt ggagaagcaa
     3721 taagaatgcc ggttggggtt gcgatgatga cgaccacgac aactggtgtc attatttaag
     3781 ttgccgaaag aacctgagtg catttgcaac atgagtatac tagaagaatg agccaagact
     3841 tgcgagacgc gagtttgccg gtggtgcgaa caatagagcg accatgacct tgaaggtgag
     3901 acgcgcataa ccgctagagt actttgaaga ggaaacagca atagggttgc taccagtata
     3961 aatagacagg tacatacaac actggaaatg gttgtctgtt tgagtacgct ttcaattcat
     4021 ttgggtgtgc actttattat gttacaatat ggaagggaac tttacacttc tcctatgcac
     4081 atatattaat taaagtccaa tgctagtaga gaaggggggt aacacccctc cgcgctcttt
     4141 tccgattttt ttctaaaccg tggaatattt cggatatcct tttgttgttt ccgggtgtac
     4201 aatatggact tcctcttttc tggcaaccaa acccatacat cgggattcct ataatacctt
     4261 cgttggtctc cctaacatgt aggtggcgga ggggagatat acaatagaac agataccaga
     4321 caagacataa tgggctaaac aagactacac caattacact gcctcattga tggtggtaca
     4381 taacgaacta atactgtagc cctagacttg atagccatca tcatatcgaa gtttcactac
     4441 cctttttcca tttgccatct attgaagtaa taataggcgc atgcaacttc ttttcttttt
     4501 ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa aaaaatgatg
     4561 gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc gttgttccag
     4621 agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt cacgcacact
     4681 actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt gaaataaaaa
     4741 aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc ttttgtttcc
     4801 tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata tttcaagcta
     4861 taccaagcat acaatcaact ccaagctttg caaagatgga taaagcggaa ttaattcccg
     4921 agcctccaaa aaagaagaga aaggtcgaat tgggtaccgc cgccaatttt aatcaaagtg
     4981 ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc
     5041 tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc
     5101 atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa
     5161 aaccactgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag
     5221 ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat gatgaagata
     5281 ccccaccaaa cccaaaaaaa gagatcttta atacgactca ctatagggcg agcgccgcca
     5341 tggagtaccc atacgacgta ccagattacg ctcatatggc catggaggcc agtgaattcc
     5401 acccgggtgg gcatcgatac gggatccgag gagatgccac catgccggcc gtatccaagg
     5461 gggaggggat gcgaggccta gcggtcttca tctccgatat ccgtaactgt aaaagtaaag
     5521 aagctgaaat aaagaggata aataaggagc tggcaaatat tagatcaaaa tttaaaggtg
     5581 acaaggctct tgatggctac agtaagaaga agtatgtctg caagttactc ttcatctttc
     5641 tccttggtca tgacattgac ttcggacaca tggaagctgt gaatcttctg agctcaaaca
     5701 gatacacgga aaagcagatc ggctaccttt tcatctctgt attggtgaat tcaaatagtg
     5761 aactgatccg gttgattaac aatgccatca agaatgacct ggccagccgc aatcctacat
     5821 ttatgggtct ggcgctgcac tgcatcgcca atgtgggtag ccgtgagatg gcagaggcct
     5881 tcgctggaga gatccccaag atcctggtgg caggagatac catggacagt gtgaagcaga
     5941 gcgcagccct gtgcttgttg cgcctgtaca ggacgtcacc tgacctagtt cctatgggcg
     6001 actggacatc ccgtgtggta cacctcctca atgaccagca cttgggtgta gtgactgccg
     6061 caactagcct catcaccaca ctggcccaga agaatcctga ggagttcaag acctctgtct
     6121 ctctagctgt ctctcgactg agcagaattg tgacgtccgc gtcaacagat cttcaggatt
     6181 atacttacta ctttgtcccg gctccttggc tgtcagtcaa acttctgaga ctgctgcagt
     6241 gctacccacc cccagaccct gcggtgcgtg gccgcctgac tgagtgcttg gagaccatcc
     6301 tgaataaggc ccaggagccc cccaagtcca agaaagtcca gcattcaaat gccaagaacg
     6361 ctgtgctgtt tgaggccatc agcctcatca tccaccatga cagtgagccc aacctcctgg
     6421 tccgtgcctg caaccagctg ggccagttcc tgcagcatcg ggagacaaac ctgcgctacc
     6481 tggctctgga gagcatgtgc acactggcca gctctgagtt ctctcacgag gctgtcaaga
     6541 cccacatcga gactgtcatc aatgccttga agacggaacg agatgtgagt gtgcggcagc
     6601 gggcagtgga cctgctctac gccatgtgcg accgaagcaa cgcccagcag attgtggctg
     6661 agatgctgag ctacctagaa acagctgact actccatccg ggaggaaatt gtgctgaagg
     6721 tggccatcct tgctgagaag tatgcagtgg actacacttg gtatgtggac accatcctca
     6781 acctcatccg cattgcaggc gactatgtga gcgaagaggt gtggtaccgc gtcatccaga
     6841 ttgtcatcaa tcgcgatgat gtgcagggtt atgctgccaa gacggtgttt gaggcattgc
     6901 aggctccagc atgtcatgag aacttggtca aagtgggcgg ctacatcctg ggcgagtttg
     6961 gaaacttgat agcgggagac ccacgatcca gccctctgat ccagttcaac ctgctccact
     7021 ccaagttcca cttgtgcagc gtccccactc gggcactcct gctgtccacc tacatcaagt
     7081 tcgtgaacct ctttccagag gtaaaggcta ccattcagga cgtgctgcgc agtgacagcc
     7141 aactgaagaa cgcagatgtg gagctgcagc agcgggctgt ggagtacctg cgactgagta
     7201 ctgttgccag cactgatatc ctggcaactg tcctggaaga aatgccaccc ttccctgagc
     7261 gtgagtcctc catcttggcc aagctgaaga agaagaaggg cccaagcaca gtgactgacc
     7321 tggaggagac caagcgtgag cgaagcatcg atgtgaatgg gggccccgaa cccgttccag
     7381 ccagcaccag tgctgcgtcc acaccttctc catcagcaga cctgctgggt ctgggggccg
     7441 ttccccctgc tcccacaggg ccccctccca cctctggcgg tgggttactg gtggacgtgt
     7501 tctcagactc agcttctgca gttgcacctc ttgcacccgg ctctgaagac aactttgcca
     7561 ggtttgtttg taaaaacaat ggtgtgttgt ttgaaaacca gctgcttcaa attggactga
     7621 agtcagaatt tcggcagaat ttaggtcgaa tgttcatatt ttatggtaac aagacctcca
     7681 cgcagttcct aaacttcact ccaacactaa tctgtgctga cgaccttcag actaatctga
     7741 acctgcagac taagcccgtg gacccaactg tggatggggg cgcacaggtg caacaggtga
     7801 tcaacattga gtgtatatct gatttcacag aggcacctgt cctcaacatc cagttcaggt
     7861 atggtggtac cttccagaat gtgtctgtta agcttcccat cactctcaac aaatttttcc
     7921 agcccacaga aatggcttct caggatttct ttcaacgttg gaagcagttg agcaatccac
     7981 agcaagaagt gcagaatatc ttcaaagcaa agcatccgat ggacacagaa atcactaaag
     8041 cgaagattat tggatttggt tctgcgctcc tggaagaagt tgatccaaac cctgcaaatt
     8101 ttgtgggtgc tggcataata cataccaaaa ccacccagat cggctgcttg ttgcgcctgg
     8161 agccaaacct gcaagctcag atgtacagac tcaccttgcg taccagcaaa gacaccgtct
     8221 ctcagagact atgtgaattg ctgtcggaac agttctaact cgagctgcag atgaatcgta
     8281 gatactgaaa aaccccgcaa gttcacttca actgtgcatc gtgcaccatc tcaatttctt
     8341 tcatttatac atcgttttgc cttcttttat gtaactatac tcctctaagt ttcaatcttg
     8401 gccatgtaac ctctgatcta tagaattttt taaatgacta gaattaatgc ccatcttttt
     8461 tttggaccta aattcttcat gaaaatatat tacgagggct tattcagaag ctttggactt
     8521 cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga
     8581 aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa
     8641 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     8701 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     8761 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     8821 cacctctacc ggccggtcga aattccccta ccctatgaac atattccatt ttgtaatttc
     8881 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
     8941 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
     9001 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
     9061 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
     9121 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
     9181 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
     9241 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
     9301 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
     9361 gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt
     9421 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
     9481 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
     9541 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
     9601 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
     9661 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
     9721 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
     9781 ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc
     9841 ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcttc gatagcagca
     9901 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
     9961 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
    10021 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc
    10081 tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa
    10141 aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc
    10201 atgaacgctt ctctattcta tatgaaaagc cggttccggc gctctcacct ttcctttttc
    10261 tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa
    10321 tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc gttatgttga
    10381 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt
    10441 cccacagttg ggggatctcg actctagcta gaggatcaat tcgtaatcat gtcatagctg
    10501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    10561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    10621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctga taacttcgta taatgtatgc
    10681 tatacgaagt tatcagctgc a
//
