LOCUS       sequence-388896-       13440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and 
            blasticidin resistance: EFS 
            promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH
  TITLE     Deep learning models to predict the editing efficiencies and 
            outcomes of diverse base editors.
  JOURNAL   Nat Biotechnol. 2024 Mar;42(3):484-497. doi: 
            10.1038/s41587-023-01792-x
  PUBMED    37188916
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2024-03"; volume: "42"; issue: "3"; pages: 
            "484-497"
COMMENT     Sequence Label: SpCas9-NRCH-ABE8.17-m+V106W
FEATURES             Location/Qualifiers
     source          1..13440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     686..706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             749..929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    976..1101
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1594..1827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2012..2056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2354..2471
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2564..2775
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2793..3290
                     /codon_start=1
                     /product="DNA adenosine deaminase evolved from the E. coli 
                     tRNA adenosine deaminase TadA (Gaudelli et al., 2017)"
                     /label=TadA*(7.10)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYSTFEPCVMCAGAMIHSRIGRVVFG
                     WRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRRVFNAQKKAQS
                     STD"
     CDS             3387..7487
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMVKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQGDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRLRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKGNSDKLIARKKDWDPKKYGGFNSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTINRKQYNTTKEVLD
                     ATLIRQSITGLYETRIDLSQLGGD"
     CDS             7533..7553
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7554..7577
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7587..7643
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7644..8042
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8067..8655
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8120..8140)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8538..8549)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8658..8674)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8659..8675)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9180..9360
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9386..9403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9392..9616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9662..10090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9749..9768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9959..9980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10099..10119)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10264..10398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10325..10344
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10460..10507
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10526..10900
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11030..11151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11067..11086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11121..11140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11200..11216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11200..11216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11213..11235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11224..11240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11248..11254,11255..11272,11273..11278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11293..11314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11431..11448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11602..12190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11682..11701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12361..13152,13153..13221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12984..13003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13222..13326)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13401..13420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
       61 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      121 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      721 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      901 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      961 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1021 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1081 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1141 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1201 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1261 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1321 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1381 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1441 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1501 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1561 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1621 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1681 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1741 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1801 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1861 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1921 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1981 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2041 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2101 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2161 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2221 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2281 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2341 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2401 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2461 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attagctagc
     2521 taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     2581 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg
     2641 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     2701 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     2761 gccgccagaa cacaggaccg gttgccacca tgtccgaagt cgagttttcc catgagtact
     2821 ggatgagaca cgcattgact ctcgcaaaga gggctcgaga tgaacgcgag gtgcccgtgg
     2881 gggcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatagg gcaatcggac
     2941 tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggaggg cttgtgatgc
     3001 agaattatcg acttatcgat gcgacgctgt actcgacgtt tgaaccttgc gtaatgtgcg
     3061 cgggagctat gattcactcc cgcattggac gagttgtatt cggttggcgc aacgccaaga
     3121 cgggtgccgc aggttcactg atggacgtgc tgcattaccc aggcatgaac caccgggtag
     3181 aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgttac ttttttcgca
     3241 tgcccaggcg tgtctttaac gcccagaaaa aagcacaatc ctctactgac tctggtggtt
     3301 cttctggtgg ttctagcggc agcgagactc ccgggacctc agagtccgcc acacccgaaa
     3361 gttctggtgg ttcttctggt ggttctgaca agaagtacag catcggcctg gccatcggca
     3421 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
     3481 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
     3541 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
     3601 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
     3661 tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc
     3721 acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc
     3781 ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc
     3841 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
     3901 acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca
     3961 accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt
     4021 ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga
     4081 agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca
     4141 agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg
     4201 acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg
     4261 ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca
     4321 ccaaggcccc cctgagcgcc tctatggtga agagatacga cgagcaccac caggacctga
     4381 ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg
     4441 accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct
     4501 acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc
     4561 tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcatc atcccccacc
     4621 agatccacct gggagagctg cacgccattc tgcggcggca gggcgatttt tacccattcc
     4681 tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg
     4741 gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca
     4801 tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg
     4861 agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc
     4921 tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg
     4981 gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
     5041 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
     5101 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
     5161 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
     5221 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
     5281 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
     5341 ggctgagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
     5401 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
     5461 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
     5521 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
     5581 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcg
     5641 gccacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
     5701 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
     5761 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
     5821 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     5881 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
     5941 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
     6001 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     6061 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     6121 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     6181 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     6241 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     6301 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
     6361 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     6421 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     6481 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     6541 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     6601 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     6661 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     6721 agggcaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     6781 gcttcaacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     6841 agtccaagaa actgaagagt gtgaaagagc tactggggat caccatcatg gaaagaagca
     6901 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     6961 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     7021 tgctggcctc tgccggcgtg ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     7081 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     7141 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     7201 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     7261 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     7321 ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc accatcaacc
     7381 ggaagcagta caacaccacc aaagaggtgc tggacgccac cctgatccgg cagagcatca
     7441 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacgag ggagctgata
     7501 agcgcaccgc cgatggttcc gagttcgaaa gccccaagaa gaagaggaaa gtcgattaca
     7561 aagacgatga cgataaggga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     7621 atgtcgaaga gaatcctgga ccgatggcca agcctttgtc tcaagaagaa tccaccctca
     7681 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     7741 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     7801 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     7861 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     7921 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     7981 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     8041 aagaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt tgtgaaagat
     8101 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8161 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8221 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     8281 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8341 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8401 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8461 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8521 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8581 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8641 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     8701 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     8761 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     8821 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8881 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     8941 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     9001 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     9061 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     9121 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     9181 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9241 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9301 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9361 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     9421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     9481 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     9541 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     9601 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     9661 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9841 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9961 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10081 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    10141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    10201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    10261 tagtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10321 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
    10381 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
    10441 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
    10501 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
    10561 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
    10621 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
    10681 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
    10741 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
    10801 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
    10861 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
    10921 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    10981 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    11041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    11101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    11161 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    11221 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11281 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11341 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11401 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11461 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11521 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11581 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11641 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11701 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11761 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11821 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11881 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11941 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    12001 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    12061 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    12121 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12181 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12241 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    12301 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    12361 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12421 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12481 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    12541 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    12601 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12661 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12721 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12781 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12841 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12901 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12961 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    13021 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    13081 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    13141 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    13201 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    13261 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    13321 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    13381 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
//
