LOCUS       sequence-388901-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GCaMP8f in a Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Moffa JC, Bland IN, Tooley JR, Kalyanaraman V, Heitmeier M, Creed 
            MC, Copits BA
  TITLE     Cell-Specific Single Viral Vector CRISPR/Cas9 Editing and 
            Genetically Encoded Tool Delivery in the Central and Peripheral 
            Nervous Systems.
  JOURNAL   eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.2024. doi: 
            10.1523/ENEURO.0438-23.2024. Print 2024 Jul.
  PUBMED    38871457
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/ENEURO.0438-23.2024"; journalName: "eNeuro"; date: 
            "2024-07-5- 5"; volume: "11"; issue: "7"; pages: 
            "ENEURO.0438-23.2024"
COMMENT     Sequence Label: pX552-hsyn DIO GCaMP8f(with gRNA scaffold)
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    179..212
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    275..308
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     893..912
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctnctnntc tgcggngggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa
       61 ggtaccggat ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
      121 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccccat
      181 aacttcgtat aaagtatcct atacgaagtt atatcaaaat aggaagacca atgcttcacc
      241 atcgacccga attgccaagc atcaccatcg acccataact tcgtataatg tatgctatac
      301 gaagttatga attcctgcag gagctcacta gtttcacttc gctgtcatca tttgtacaaa
      361 ctcttcgtag tttacctgac catccccatc gatgtctgct tccctgatca tttcatcaac
      421 ctcttcatct gttaacttct ctccaaggtt tgtcatcacg tggcgaagct ctgctgcact
      481 gatgtagcca ttgccatcct tatcaaacac accgaacgct tctctaattt cttcttccgt
      541 gtccctgtat ttcatttttc ttgccatcat tgtcaggaac tcagggaagt cgattgtgcc
      601 gtcaccgtcg gcatctactt cattgatcat gtcctgcagc tctgcttctg tggggttcag
      661 tccgagagac cgcatcaccg tccccagctc cttggttgtt attgtcccat ccccgtcctt
      721 gtcaaatagg gagaaagcct ctttaaattc tgcgatctgc tctccagtca gttggtccgg
      781 caggttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      841 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      901 gttgccgtcg tccttgaaga anatggtgcg ctcctggatg tagccttcgg gcatggcgna
      961 cttgaanaan tcgtgctgct tcatgtggtc ggggtagcgg ctgancactg cacgccgtag
     1021 ncnnntngnc acnnnntggg nnnngnanng ncnncttncn ggtggnngca natnaacttc
     1081 agggnnannn ttgccgnnnn gnngnnat
//
