LOCUS       sequence-388902-       12859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized Sc++ and blasticidin resistance: EFS
            promoter-Sc++-NLS-FLAG-P2A-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH
  TITLE     Deep learning models to predict the editing efficiencies and 
            outcomes of diverse base editors.
  JOURNAL   Nat Biotechnol. 2024 Mar;42(3):484-497. doi: 
            10.1038/s41587-023-01792-x
  PUBMED    37188916
REFERENCE   2  (bases 1 to 12859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2024-03"; volume: "42"; issue: "3"; pages: 
            "484-497"
COMMENT     Sequence Label: Sc++
FEATURES             Location/Qualifiers
     source          1..12859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        165..544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        546..744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     699..719
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             762..942
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    989..1114
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1607..1840
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2025..2069
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2367..2484
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2577..2788
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2807..2816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6938..6985
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6986..7009
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7019..7075
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7076..7474
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7499..8087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7552..7572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7970..7981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8090..8106)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8091..8107)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8612..8792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8818..8835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8824..9048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9094..9522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9181..9200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9391..9412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9531..9551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9696..9830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9757..9776
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9892..9939
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9958..10332
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10462..10583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10499..10518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10553..10572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10632..10648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10632..10648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10645..10667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10656..10672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10680..10686,10687..10704,10705..10710))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10725..10746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10863..10880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11034..11622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11114..11133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11793..12584,12585..12653))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12416..12435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12654..12758)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12833..12852)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
       61 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      121 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      181 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      241 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      301 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      361 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      421 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      481 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      541 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      601 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      661 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      721 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
      781 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      841 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      901 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      961 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1021 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1081 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1141 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1201 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1261 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1321 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1381 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1441 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1501 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1561 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1621 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1681 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1741 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1801 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1861 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1921 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1981 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2041 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2101 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2161 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2221 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2281 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2341 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2401 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2461 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2521 ttaattagct agctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     2581 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg
     2641 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2701 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2761 tcgcaacggg tttgccgcca gaacacagga ccggttctag agcgctgcca ccatggaaaa
     2821 gaaatatagc attgggctgg acattggaac aaactccgtg ggctgggctg tgattactga
     2881 tgattacaaa gtgccttcaa agaagtttaa ggtgctgggc aacaccaata gaaagtctat
     2941 caagaagaac ctgatgggcg ccctgctgtt cgacagcgga gagacagccg aggcaacccg
     3001 gctgaagaga acagcccgga gaaggtacac ccgccggaag aaccggatca gatacctcca
     3061 ggagattttc gccaatgaga tggccaagct ggacgatagc ttctttcaga gactggagga
     3121 gtccttcctg gtggaggagg acaagaagaa cgagaggcac ccaatctttg gcaatctggc
     3181 cgatgaggtg gcctatcaca gaaattaccc taccatctat cacctgagga agaagctggc
     3241 cgatagccca gagaaggccg acctgcgcct gatctacctg gccctggccc acatcatcaa
     3301 gttccggggc cactttctga tcgagggcaa gctgaacgcc gagaacagcg acgtggccaa
     3361 gctgttctac cagctgatcc agacatataa ccagctgttt gaggagtctc ccctggatga
     3421 gatcgaggtg gacgccaagg gcatcctgag cgccagactg tctaagagca agaggctgga
     3481 gaagctgatc gccgtgttcc caaacgagaa gaagaatggc ctgtttggca atatcatcgc
     3541 cctggccctg ggcctgaccc caaacttcaa gtccaatttt gacctgacag aggatgccaa
     3601 gctccagctg tctaaggata cctacgacga tgacctggac gagctgctgg gccagatcgg
     3661 cgaccagtat gccgatctgt tctctgccgc caagaacctg agcgatgcca tcctgctgtc
     3721 cgacatcctg agatccaatt ctgaggtgac aaaggcccct ctgagcgcca gcatggtgaa
     3781 gcggtacgat gagcaccacc aggacctggc cctgctgaag accctggtgc ggcagcagtt
     3841 cccagagaag tatgccgaga ttttcaagga tgacacaaag aacggctacg ccggctatgt
     3901 gggcgccgac aagaagctga ggaagcgcag cggcaagctg gccaccgagg aggagttcta
     3961 caagtttatc aagcctatcc tggagaagat ggacggagca gaggagctgc tggccaagct
     4021 gaacagggat gacctgctga ggaagcagcg cacatttgat aatggcagca tccctcacca
     4081 gatccacctg aaggagctgc acgccatcct gagaaggcag gaggagttct acccatttct
     4141 gaaggagaac agagagaaga tcgagaagat cctgaccttc agaatcccct actatgtggg
     4201 acctctggcc aggggcaact ctcgctttgc ctggctgacc agaaagagcg aggaggccat
     4261 cacaccctgg aatttcgagg aggtggtgga caagggagcc agcgcccagt ccttcatcga
     4321 gaggatgaca aactttgatg agcagctgcc aaataagaag gtgctgccca agcactccct
     4381 gctgtacgag tatttcacag tgtacaatga gctgaccaag gtgaagtatg tgacagagcg
     4441 gatgagaaag cctgagttcc tgtctggcga gcagaagaag gccatcgtgg acctgctgtt
     4501 taagacaaac cgcaaggtga ccgtgaagca gctgaaggag gattacttca agaagatcga
     4561 gtgctttgac agcgtggaga tcatcggcgt ggaggatcgg tttaatgcct ccctgggcac
     4621 ctatcacgac ctgctgaaga tcatcaagga taaggacttc ctggataacg aggagaatga
     4681 ggacatcctg gaggacatcg tgctgaccct gaccctgttt gaggaccgcg agatgatcga
     4741 ggagcggctg aagacctacg cccacctgtt cgatgacaaa gtgatgaagc agctgaagcg
     4801 ccggcactat acaggatggg gccggctgag cagaaagatg atcaacggca tcagagataa
     4861 gcagtccggc aagaccatcc tggattttct gaagtctgac ggcttcagca acaggaactt
     4921 catgcagctg atccacgatg acagcctgac attcaaggag gagatcgaga aggcccaggt
     4981 gtctggccag ggcgatagcc tgcacgagca gatcgccgac ctggcaggct ccccagccat
     5041 caagaagggc atcctccaga ccgtgaagat cgtggacgag ctggtgaaag tgatgggcca
     5101 caagcccgag aacatcgtga tcgagatggc ccgcgagaat cagaccacaa ccaagggcct
     5161 ccagcagtcc agggagcgca agaagcggat cgaggagggc atcaaggagc tggagtctca
     5221 gatcctgaag gagaaccccg tggagaatac ccagctccag aacgagaagc tgtacctgta
     5281 ctacctccag aatggcagag acatgtatgt ggatcaggag ctggacatca acaggctgag
     5341 cgattacgac gtggatcaca tcgtgcccca gtctttcatc aaggatgaca gcatcgacaa
     5401 taaggtgctg acacggtccg tggagaacag gggcaagtcc gataatgtgc cttctgagga
     5461 ggtggtgaag aagatgaaga actattggcg ccagctgctg aatgccaagc tgatcaccca
     5521 gcgcaagttt gacaacctga caaaggcaga gaggggaggc ctgtctgagg cagataaggc
     5581 cggcttcatc aagagacagc tggtggagac aaggcagatc accaagcacg tggccagaat
     5641 cctggacagc cggatgaaca ccaagcggga caagaatgat aagcctatcc gggaggtgaa
     5701 agtgatcacc ctgaagtcca agctggtgtc tgatttccgc aaggactttc agctgtacaa
     5761 ggtgcgggac atcaacaatt accaccacgc ccacgatgcc tatctgaacg ccgtggtggg
     5821 caccgccctg atcaagaagt atccaaagct ggagagcgag ttcgtgtacg gcgattataa
     5881 ggtgtacgac gtgagaaaga tgatcgccaa gtctgagcag gagatcggca aggccaccgc
     5941 caagaggttc ttttacagca acatcatgaa tttctttaag acagaggtga agctggccaa
     6001 cggagagatc aggaagcggc ccctgatcga gacaaacggc gagacaggcg aggtggtgtg
     6061 gaataaggag aaggactttg ccaccgtgag aaaggtgctg gccatgcccc aagtgaatat
     6121 cgtgaagaag acagaggtgc agaccggcgg cttctctaag gagagcatcc tgtccaagag
     6181 ggagtccgcc aagctgatcc cccgcaagaa gggctgggat acccggaagt acggcggctt
     6241 tggctcccct acagtggcct attctatcct ggtggtggcc aaggtggaga agggcaaggc
     6301 caagaagctg aagtccgtga aggtgctggt gggcatcacc atcatggaga agggctctta
     6361 cgagaaggac cccatcggct tcctggaggc caagggctat aaggacatca agaaggagct
     6421 gatcttcaag ctgcctaagt acagcctgtt tgagctggag aacggcagaa ggcgcatgct
     6481 ggcctccgcc aaggagctcc agaaggccaa tgagctggtg ctgccacagc acctggtgcg
     6541 cctgctgtac tatacacaga acatcagcgc cacaaccggc tccaacaatc tgggctacat
     6601 cgagcagcac cgggaggagt tcaaggagat tttcgagaag atcatcgact tctccgagaa
     6661 gtatatcctg aagaacaagg tgaactctaa tctgaagagc tccttcgatg agcagtttgc
     6721 cgtgtctgac agcatcctgc tgtccaattc tttcgtgtcc ctgctgaagt acaccagctt
     6781 tggcgcctcc ggcggcttca catttctgga cctggatgtg aagcagggca ggctgcgcta
     6841 tcagacagtg accgaggtgc tggatgccac cctgatctac cagagcatta ccggactgta
     6901 tgaaactcgg actgacctga gccagctggg gggggacaag cgacctgccg ccacaaagaa
     6961 ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg gatccggcgc
     7021 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatggc
     7081 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     7141 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     7201 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     7261 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     7321 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     7381 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     7441 attgctgccc tctggttatg tgtgggaggg ctaagaattc gatatcaagc ttatcggtaa
     7501 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7561 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7621 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7681 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7741 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7801 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7861 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7921 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7981 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8041 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     8101 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     8161 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     8221 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8281 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     8341 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     8401 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     8461 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     8521 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     8581 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     8641 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8701 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8761 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     8821 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     8881 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     8941 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9001 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9061 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9121 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9181 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9241 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9301 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9361 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9541 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     9721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9781 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9841 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     9901 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     9961 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
    10021 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
    10081 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
    10141 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
    10201 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
    10261 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
    10321 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
    10381 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10441 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    10501 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10561 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    10621 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10681 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10741 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10801 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10921 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11041 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11101 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11221 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11281 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11341 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11401 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11461 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11521 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11581 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11641 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11701 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11761 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11821 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11881 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11941 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    12001 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12061 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    12121 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12181 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12241 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12301 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12361 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12421 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12481 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12541 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12601 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12661 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12721 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12781 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    12841 gctctgatgc cgcatagtt
//
